LOCUS       sequence-400836-       11364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes LightsOut (AsLOV2(408-543) inserted in Gal4 SK22) 
            transcription factor in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11364)
  AUTHORS   Zhu L, McNamara HM, Toettcher JE
  TITLE     Light-switchable transcription factors obtained by direct screening 
            in mammalian cells.
  JOURNAL   Nat Commun. 2023 Jun 2;14(1):3185. doi: 10.1038/s41467-023-38993-6.
  PUBMED    37268649
REFERENCE   2  (bases 1 to 11364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-06-2"; volume: "14(1):3185. doi"; pages: " 
            10.1038/s41467-023-38993-6"
COMMENT     Sequence Label: pSFFV_Gal4LOV[SK22]-VP64-IRES-mCherry (pLZA140)
FEATURES             Location/Qualifiers
     source          1..11364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             249..293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    578..695
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        848..1255
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2234..2383
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     misc_feature    2409..2982
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2432..2984
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2576..2593)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2803..2822
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2984..3694
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3129..3147)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3397..3416
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3756..4344
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3809..3829)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4227..4238)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4347..4363)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4348..4364)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4454..4687
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4712..4730)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4713..4730)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5020..5039)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5253..5272)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5372..5394
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5432..5450)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5518..5622
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5623..5691,5692..6483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5841..5860)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6654..7242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7143..7162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7396..7413
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7483..7503)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7488..7817
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7668..7803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8951..9016
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9146..9166
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9591..9725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9628..9647)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9682..9701
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9893..10526
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10573..10698
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(11195..11364,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      601 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      661 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      721 atccagtttg gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat
      781 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
      841 agctgcagta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
      901 cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg
      961 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     1021 ccgaggccaa gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     1081 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     1141 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     1201 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     1261 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc
     1321 ggtcgccacc atgaagctgc tgagcagcat cgagcaggcc tgtgacatct gccggctgaa
     1381 gaaactgaag tgcagtgcat ctttggaacg tatcgaaaag aattttgtca tcacggatcc
     1441 gcgtcttccc gacaatccga ttatcttcgc gtcagactct ttcttacaac tgactgagta
     1501 tagtagagag gagatattgg ggcgtaactg tagatttctt caggggccag aaactgatcg
     1561 ggctaccgtt cgcaagatac gtgacgcaat agacaaccag accgaggtga cggtgcagct
     1621 gattaactac acaaagtctg ggaagaagtt ctggaacctg tttcatttac aacctatgag
     1681 agatcaaaaa ggtgacgttc aatatttcat cggggttcag ttagatggga ctgagcacgt
     1741 gagagatgca gcagaaagag agggtgtaat gcttattaaa aaaacagccg agaatatcga
     1801 cgaagccgct gcgtcatgca gcaaagaaaa gcccaagtgc gccaagtgcc tgaagaacaa
     1861 ctgggagtgc cggtacagcc ccaagaccaa gagaagcccc ctgaccagag cccacctgac
     1921 cgaggtggaa agccggctgg aaagactgga acagctgttt ctgctgatct tcccacgcga
     1981 ggacctggac atgatcctga agatggacag cctgcaggac atcaaggccc tgctgaccgg
     2041 cctgttcgtg caggacaacg tgaacaagga cgccgtgacc gacagactgg ccagcgtgga
     2101 aaccgacatg cccctgaccc tgcggcagca cagaatcagc gccaccagca gcagcgagga
     2161 aagcagcaac aagggccagc ggcagctgac agtgtctgct gctgcaggcg gaagcggagg
     2221 ctctggcgga tctgatgccc tggacgactt cgacctggat atgctgggca gcgacgccct
     2281 ggatgatttt gatctggaca tgctgggatc tgacgctctg gacgatttcg atctcgacat
     2341 gttgggatca gatgcactgg atgactttga cctggacatg ctcggatcat gagtaccaag
     2401 ctgatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     2461 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     2521 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     2581 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     2641 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     2701 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2761 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     2821 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     2881 cctcggtaca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     2941 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggtga gcaagggcga
     3001 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3061 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3121 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3181 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3241 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3301 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3361 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3421 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3481 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3541 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3601 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3661 ctccaccggc ggcatggacg agctgtacaa gtagctgagg ccgcgactct agagtcgacc
     3721 tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
     3781 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3841 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3901 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3961 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4021 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4081 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4141 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     4201 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4261 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4321 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat taattcgagc
     4381 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4441 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     4501 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4561 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4621 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4681 tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta
     4741 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta
     4801 attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca
     4861 gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa
     4921 tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg
     4981 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     5041 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     5101 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     5161 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     5221 ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     5281 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     5341 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     5401 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     5461 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     5521 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5581 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5641 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5701 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5761 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5821 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5881 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5941 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6001 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6061 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6121 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6181 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6241 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6301 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6361 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6421 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6481 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6541 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6601 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6661 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6721 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6781 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6841 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6901 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6961 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7021 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7081 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7141 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     7201 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     7261 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     7321 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     7381 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     7441 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
     7501 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7561 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7621 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7681 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7741 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7801 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
     7861 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     7921 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     7981 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     8041 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     8101 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
     8161 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     8221 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     8281 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     8341 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     8401 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     8461 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     8521 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     8581 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     8641 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     8701 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
     8761 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     8821 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
     8881 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     8941 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     9001 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     9061 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     9121 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     9181 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     9241 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     9301 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
     9361 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
     9421 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     9481 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     9541 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     9601 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9661 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9721 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     9781 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     9841 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     9901 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     9961 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
    10021 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
    10081 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
    10141 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
    10201 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
    10261 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
    10321 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
    10381 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    10441 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    10501 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    10561 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    10621 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    10681 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    10741 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    10801 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    10861 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    10921 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    10981 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    11041 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
    11101 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    11161 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
    11221 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
    11281 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
    11341 atctgttgca actcacagtc tggg
//
