LOCUS       sequence-400873-        9833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses DCK* and GFP from the same transcript.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Koduri V, Duplaquet L, Lampson BL, Wang AC, Sabet AH, Ishoey M, 
            Paulk J, Teng M, Harris IS, Endress JE, Liu X, Dasilva E, Paulo JA, 
            Briggs KJ, Doench JG, Ott CJ, Zhang T, Donovan KA, Fischer ES, Gygi 
            SP, Gray NS, Bradner J, Medin JA, Buhrlage SJ, Oser MG, Kaelin WG Jr
  TITLE     Targeting oncoproteins with a positive selection assay for protein 
            degraders.
  JOURNAL   Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6263. doi: 
            10.1126/sciadv.abd6263. Print 2021 Feb.
  PUBMED    33547076
REFERENCE   2  (bases 1 to 9833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-02-5"; volume: "7(6). pii: 7/6/eabd6263. doi"; pages: " 
            10.1126/sciadv.abd6263. Print 2021 Feb"
COMMENT     Sequence Label: pLX304-DCK*-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     168..187
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     421..438
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    555..576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(591..596,597..614,615..621)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    629..645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     634..656
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     653..669
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     653..669
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     688..708
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        690..708
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        736..962
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             963..1143
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1190..1315
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1808..2041
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2226..2270
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2445..2945
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2864..2882
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2957..3352
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    3412..3529
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3646..3949
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3950..4153
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4104..4124
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    4176..4200
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4984..5008)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5010..5051
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(5092..5666,5667..5669,5670..5678)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5259..5276)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5486..5505
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5679..5681,5682..5684,5685..6398)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5679..5681,5682..5684,5685..6395)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5724..5745)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5985..6004)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6332..6353
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6421..7009
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6474..6494)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6892..6903)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7081..7314
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7392..7513
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7429..7448)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7483..7502
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7553..7688
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7708..7727)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7709..7727)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7737..7754)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7737..7753)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7746..7768)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7895..8350
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7982..8001)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8192..8213
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8376..8480
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8481..8549,8550..9341)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8699..8718)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9512..9833,1..267)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
       61 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
      121 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
      181 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
      241 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
      301 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
      361 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
      421 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
      481 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
      541 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
      601 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      661 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
      721 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
      781 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
      841 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
      901 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
      961 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     1021 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     1081 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1141 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     1201 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1261 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1321 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1381 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1441 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1501 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1561 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1621 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1681 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1741 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1801 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1861 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1921 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1981 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     2041 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     2101 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     2161 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2221 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2281 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2341 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2401 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
     2461 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     2521 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     2581 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     2641 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     2701 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     2761 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     2821 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     2881 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     2941 cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     3001 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     3061 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     3121 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     3181 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     3241 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     3301 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gcctgcagct
     3361 gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     3421 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     3481 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     3541 cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta
     3601 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     3661 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     3721 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     3781 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     3841 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     3901 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     3961 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     4021 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     4081 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     4141 tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa aaagcaggct
     4201 ctatggccac cccgcccaag agaagctgcc cgtctttctc agccagctct gaggggaccc
     4261 gcatcaagaa aatctccatc gaagggaaca tcgctgcagg gaagtcaaca tttgtgaata
     4321 tccttaaaca attgtgtgaa gattgggaag tggttcctga acctgttgcc agatggtgca
     4381 atgttcaaag tactcaagat gaatttgagg aacttacaat ggagcagaaa aatggtggga
     4441 atgttcttca gatgatgtat gagaaacctg aacgatggtc ttttaccttc caaacatatg
     4501 cctgtctcag tatgataaga gctcagcttg cctctctgaa tggcaagctc aaagatgcag
     4561 agaaacctgt attatttttt gaacgatctg tgtatagtgc gaggtatatt tttgcatcta
     4621 atttgtatga atctgaatgc atgaatgaga cagagtggac aatttatcaa gactggcatg
     4681 actggatgaa taaccaattt ggccaaagcc ttgaattgga tggaatcatt tatcttcaag
     4741 ccactccaga gacatgctta catagaatat atttacgggg aagaaatgaa gagcaaggca
     4801 ttcctcttga atatttagag aagcttcatt ataaacatga aagctggctc ctgcatagga
     4861 cactgaaaac caacttcgat tatcttcaag aggtgcctat cttaacactg gatgttaatg
     4921 aagactttaa agacaaatat gaaagtctgg ttgaaaaggt caaagagttt ttgagtactt
     4981 tgaacccagc tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
     5041 tcgattctac gtagtaatga gctagccgct acgtaaattc cgcccccccc ccccctctcc
     5101 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     5161 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     5221 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     5281 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     5341 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     5401 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     5461 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     5521 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     5581 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     5641 tttcctttga aaaacacgat gataatatgg ccacaaccat ggtgagcaag ggcgaggagc
     5701 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     5761 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     5821 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     5881 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     5941 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     6001 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     6061 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     6121 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     6181 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     6241 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     6301 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     6361 ccgggatcac tctcggcatg gacgagctgt acaagtaaac cggtggcgcg ttaagtcgac
     6421 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     6481 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     6541 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     6601 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     6661 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6721 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6781 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6841 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6901 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6961 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     7021 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     7081 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7141 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7201 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7261 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     7321 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7381 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7501 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     7561 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7621 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7681 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     7741 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7801 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7861 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     7921 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7981 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8041 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8101 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8161 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8221 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     8281 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     8341 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     8401 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     8461 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     8521 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     8581 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     8641 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     8701 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     8761 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     8821 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     8881 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     8941 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     9001 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     9061 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     9121 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     9181 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     9241 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     9301 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     9361 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     9421 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     9481 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     9541 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     9601 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     9661 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     9721 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     9781 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acg
//
