LOCUS       sequence-400876-        2705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mutant of PadR; transcriptional regulator can respond to p-coumaric 
            acid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Jiang T, Li C, Yan Y
  TITLE     Optimization of a p-Coumaric Acid Biosensor System for Versatile 
            Dynamic Performance.
  JOURNAL   ACS Synth Biol. 2021 Jan 15;10(1):132-144. doi: 
            10.1021/acssynbio.0c00500. Epub 2020 Dec 30.
  PUBMED    33378169
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.0c00500"; journalName: "ACS Synth Biol"; date: 
            "2021-01-15- 15"; volume: "10"; issue: "1"; pages: "132-144"
COMMENT     Sequence Label: pCS-lpp0.2-K64A
FEATURES             Location/Qualifiers
     source          1..2705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             134..145
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      730..816
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(851..868)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(985..1530)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(1065..1084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1644..1738)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1769..2563)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1880..1899)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2490..2509
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcacc tcgagatcaa aaaaatattg acaacataaa aaactttgtg ttgctggtgt
      121 aacggaattc attaaagagg agaaaggtac catgagagta ttaaaatacg ccatattagg
      181 gcttttgcga aaaggcgaat tgagtggata cgatattacg agttatttta aagaggagct
      241 cggccagttt tggagcgcca agcacagcca gatttaccct gagctcaaaa agctgacgga
      301 tgaaggattt atcacgttcc ggacaacgat tcagggcaca gccctggaga aaaagatgta
      361 caccctgaca gacagcggaa agcaggagct gcatgactgg ctgatccgcc accagccgat
      421 acccgagacg gtgaaggatg aatttatgct gaaggcttat ttcatttctt gtctatcgcg
      481 ccaggaggct tctgatttgt tcaaagacca gctgcaaaaa cgccaggcca agctttctga
      541 cttacaggga agctatgaaa agcttatggc ttcagcagag ccgatgtcat tttcttcacc
      601 ggacttcggc cactatcttg tgctaacgaa agcgctggag cgggagaaaa attacgtttc
      661 ttggctggaa tcaattttag ctatgataga taaggattaa ggatcccatg gtacgcgtgc
      721 tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      781 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgccctagac ctagggatat
      841 attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa
      901 tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt aacagggaag
      961 tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca agcatcacga
     1021 aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1081 tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     1141 tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc gggtaggcag
     1201 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc tgcgccttat
     1261 ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca ctggcagcag
     1321 ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta aggctaaact
     1381 gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt caaagagttg
     1441 gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa
     1501 gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca gataaaatat
     1561 ttctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
     1621 tataagttgt tactagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     1681 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     1741 agcgagctct cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg
     1801 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     1861 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     1921 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     1981 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct
     2041 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     2101 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     2161 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     2221 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     2281 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     2341 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac
     2401 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
     2461 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
     2521 aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatcctcatc
     2581 ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca
     2641 tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg
     2701 acgtc
//
