LOCUS       sequence-400893-        6249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rAAV9 cap acceptor vector for CREATE libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Chuapoco MR, Flytzanis NC, Goeden N, Christopher Octeau J, Roxas KM,
            Chan KY, Scherrer J, Winchester J, Blackburn RJ, Campos LJ, Man KNM,
            Sun J, Chen X, Lefevre A, Singh VP, Arokiaraj CM, Shay TF, 
            Vendemiatti J, Jang MJ, Mich JK, Bishaw Y, Gore BB, Omstead V, 
            Taskin N, Weed N, Levi BP, Ting JT, Miller CT, Deverman BE, Pickel 
            J, Tian L, Fox AS, Gradinaru V
  TITLE     Adeno-associated viral vectors for functional intravenous gene 
            transfer throughout the non-human primate brain.
  JOURNAL   Nat Nanotechnol. 2023 Jul 10. doi: 10.1038/s41565-023-01419-x.
  PUBMED    37430038
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Nanotechnol. 2023 Jul 10. doi: 10.1038/s41565-023-01419-x."
COMMENT     Sequence Label: rAAV9-CAP-in-cis-Lox-AAV
FEATURES             Location/Qualifiers
     source          1..6249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        152..550
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      553..562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             559..1269
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(704..722)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     972..991
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3637..3648
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3802..3835)
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3857..3978
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3868..3887)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3922..3941
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    4081..4114
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     repeat_region   4127..4256
                     /label=AAV2 ITR
     primer_bind     complement(4321..4339)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4407..4511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4512..4580,4581..5372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4730..4749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5543..6131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6032..6051
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
       61 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg
      121 gccaactcca tcactagggg ttcctactag tggcctccgc gccgggtttt ggcgcctccc
      181 gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg cagcgagcgt
      241 cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta
      301 gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg
      361 gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg
      421 gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca
      481 cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat
      541 cgtcacttgg cggccgccat ggtcagcaag ggcgaggagg ataacatggc catcatcaag
      601 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      661 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      721 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcaattcat gtatggctcc
      781 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      841 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      901 gactcctcct tacaagacgg cgagttcatc tacaaagtga agctgcgcgg caccaacttc
      961 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1021 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1081 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     1141 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     1201 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1261 tacaagtaaa ggatcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     1321 gtcctttcct cctcctagga ataaaatatc tttattttca ttacatctgt gtgttggttt
     1381 tttgtgtaga tctgttcaaa tttgaactga ctaagcggct cccgccagat tttggcaaga
     1441 ttactaagca ggaagtcaag gacttttttg cttgggcaaa ggtcaatcag gtgccggtga
     1501 ctcacgagtt taaagttccc agggaattgg cgggaactaa aggggcggag aaatctctaa
     1561 aacgcccact gggtgacgtc accaatacta gctataaaag tctggagaag cgggccaggc
     1621 tctcatttgt tcccgagacg cctcgcagtt cagacgtgac tgttgatccc gctcctctgc
     1681 gaccgctagc ttcgatcaac tacgcggaca ggtaccaaaa caaatgttct cgtcacgtgg
     1741 gcatgaatct gatgctgttt ccctgcagac aatgcgagag actgaatcag aattcaaata
     1801 tctgcttcac tcacggtgtc aaagactgtt tagagtgctt tcccgtgtca gaatctcaac
     1861 ccgtttctgt cgtcaaaaag gcgtatcaga aactgtgcta cattcatcac atcatgggaa
     1921 aggtgccaga cgcttgcact gcttgcgacc tggtcaatgt ggacttggat gactgtgttt
     1981 ctgaacaata aatgacttaa accaggtatg gctgccgatg gttatcttcc agattggctc
     2041 gaggacaacc ttagtgaagg aattcgcgag tggtgggctt tgaaacctgg agcccctcaa
     2101 cccaaggcaa atcaacaaca tcaagacaac gctcgaggtc ttgtgcttcc gggttacaaa
     2161 taccttggac ccggcaacgg actcgacaag ggggagccgg tcaacgcagc agacgcggcg
     2221 gccctcgagc acgacaaggc ctacgaccag cagctcaagg ccggagacaa cccgtacctc
     2281 aagtacaacc acgccgacgc cgagttccag gagcggctca aagaagatac gtcttttggg
     2341 ggcaacctcg ggcgagcagt cttccaggcc aaaaagaggc ttcttgaacc tcttggtctg
     2401 gttgaggaag cggctaagac ggctcctgga aagaagaggc ctgtagagca gtctcctcag
     2461 gaaccggact cctccgcggg tattggcaaa tcgggtgcac agcccgctaa aaagagactc
     2521 aatttcggtc agactggcga cacagagtca gtcccagacc ctcaaccaat cggagaacct
     2581 cccgcagccc cctcaggtgt gggatctctt acaatggctt caggtggtgg cgcaccagtg
     2641 gcagacaata acgaaggtgc cgatggagtg ggtagttcct cgggaaattg gcattgcgat
     2701 tcccaatggc tgggggacag agtcatcacc accagcaccc gaacctgggc cctgcccacc
     2761 tacaacaatc acctctacaa gcaaatctcc aacagcacat ctggaggatc ttcaaatgac
     2821 aacgcctact tcggctacag caccccctgg gggtattttg acttcaacag attccactgc
     2881 cacttctcac cacgtgactg gcagcgactc atcaacaaca actggggatt ccggcctaag
     2941 cgactcaact tcaagctctt caacattcag gtcaaagagg ttacggacaa caatggagtc
     3001 aagaccatcg ccaataacct taccagcacg gtccaggtct tcacggactc agactatcag
     3061 ctcccgtacg tgctcgggtc ggctcacgag ggctgcctcc cgccgttccc agcggacgtt
     3121 ttcatgattc ctcagtacgg gtatctgacg cttaatgatg gaagccaggc cgtgggtcgt
     3181 tcgtcctttt actgcctgga atatttcccg tcgcaaatgc taagaacggg taacaacttc
     3241 cagttcagct acgagtttga gaacgtacct ttccatagca gctacgctca cagccaaagc
     3301 ctggaccgac taatgaatcc actcatcgac caatacttgt actatctctc tagaactatt
     3361 accggttggg ttcaaaacca aggaatactt ccgggtatgg tttggcagga cagagatgtg
     3421 tacctgcaag gacccatttg ggccaaaatt cctcacacgg acggcaactt tcacccttct
     3481 ccgctgatgg gagggtttgg aatgaagcac ccgcctcctc agatcctcat caaaaacaca
     3541 cctgtacctg cggatcctcc aacggccttc aacaaggaca agctgaactc tttcatcacc
     3601 cagtattcta ctggccaagt cagcgtggag atcgagtggg agctgcagaa ggaaaacagc
     3661 aagcgctgga acccggagat ccagtacact tccaactatt acaagtctaa taatgttgaa
     3721 tttgctgtta atactgaagg tgtatatagt gaaccccgcc ccattggcac cagatacctg
     3781 actcgtaatc tgtaagtcga ctaccgttcg tatagcatac attatacgaa gttatcatat
     3841 gttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     3901 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     3961 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4021 gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgagctc
     4081 taccgttcgt ataatgtatg ctatacgaag ttatgatatc aagcttagga acccctagtg
     4141 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     4201 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagagag
     4261 ggagtggcca agctagcggg cgattaagga aagggctaga tcattcttga agacgaaagg
     4321 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4381 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4441 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4501 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4561 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4621 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4681 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4741 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4801 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4861 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4921 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4981 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5041 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5101 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5161 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5221 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5281 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5341 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5401 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5461 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5521 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5581 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5641 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5701 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5761 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5821 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5881 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5941 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6001 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6061 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6181 ttgctcacat gtaataaaca cacacacacc aacaaccgtg gttggttgtt gtgttggttt
     6241 attctcgag
//
