LOCUS       sequence-400925-        7678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of epithelial cell 
            SARS-CoV2 response..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, 
            Serresi M, Cicin-Sain L, Gargiulo G
  TITLE     Pharmacological modulators of epithelial immunity uncovered by 
            synthetic genetic tracing of SARS-CoV-2 infection responses.
  JOURNAL   Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. 
            Epub 2023 Jun 21.
  PUBMED    37343108
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-23"; volume: "9(25):eadf4975. doi"; pages: " 
            10.1126/sciadv.adf4975. Epub 2023 Jun 21"
COMMENT     Sequence Label: pPB-NeoCOVGT3-d2EGFP
FEATURES             Location/Qualifiers
     source          1..7678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1094..1118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1119..1128
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1125..1127,1128..1130,1131..1841,1842..1847,
                     1848..1970)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1170..1191)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1431..1450)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1778..1799
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1971..1995)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2073..2092)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2138..2193
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2192..2211)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        2548..2927
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2928..3131
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3082..3102
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3164..3958
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3164..3958
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3218..3237)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3828..3847
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3996..4013)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4002..4226
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4576..4638
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4704..4723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4705..4723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4730..4747)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4730..4746)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4739..4761)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4888..5343
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4975..4994)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5185..5206
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5369..5473
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5474..5542,5543..6334)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5692..5711)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6505..7093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6994..7013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7247..7264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7381..7402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7417..7422,7423..7440,7441..7447)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7455..7471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7460..7482
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7479..7495
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7479..7495
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7516..7534
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttggagt tgtgatcctt tggaggagaa
      361 gaggcattgt ggtttttgga attttcagca tttttgtgct ggtttttcct catctttgtg
      421 gatttatcta cctttcatct ttgatgttgg tgacctttgg atggggtttt tgcatgggca
      481 tcctttccta ctaggtttca ttttaatcct tttcaccagt tcccaaatgt aagtttcttt
      541 ttcctttctc ttttgaggca ggagatcctg ctgaactatg tattttccca ttgttatgca
      601 acttttcatt tgagttttta agaagtagta acaatatgca aaccccagtc agctaatttg
      661 ctttttgttt ttatggtttt gcctactcta gatatttcct ataaaatgga atcatacaat
      721 atgtggtccc ttgtgtctgg ctttttttac ttagcatatt ttcaaggttc atccatgttg
      781 tagcatgtat tcttccctct ggatggatac gcacatacac tggatacaca cacacacaca
      841 cacacacaca cacacacaca cacacacatt tcgcctaaca agttctatat cagactatat
      901 caggttctcc cccccgcccc ctccatgtgt cagggactat acactactgc aaataggaag
      961 ggacagtaac atttagaaga gaacaaaaca gaaactcttg gaagcaggaa aggtgcatga
     1021 ctcaaagagg gaaattcctg tgccataaaa ggattgctgg tgtataaaat gctctatata
     1081 tgccaattat ctaacaagtt tgtacaaaaa agcaggctgc caccatggtg agcaagggcg
     1141 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     1201 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     1261 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     1321 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     1381 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     1441 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     1501 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     1561 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     1621 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     1681 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     1741 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     1801 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gaagcttagc catggcttcc
     1861 cgccggaggt ggaggagcag gatgatggca cgctgcccat gtcttgtgcc caggagagcg
     1921 ggatggaccg tcaccctgca gcctgtgctt ctgctaggat caatgtgtag acccagcttt
     1981 cttgtacaaa gtggtgatcc tcaggtgcag gctgcctatc agaaggtggt ggctggtgtg
     2041 gccaatgccc tggctcacaa ataccactga gatctttttc cctctgccaa aaattatggg
     2101 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     2161 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
     2221 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
     2281 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
     2341 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
     2401 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
     2461 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
     2521 ctcgacctgc agcccaagct tcgcgttgac attgattatt gactagttat taatagtaat
     2581 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     2641 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     2701 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     2761 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     2821 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     2881 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     2941 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3001 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3061 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3121 taagcagagc tctctggcta actagagaac ccactgcgcc accatgattg aacaagatgg
     3181 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3241 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3301 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     3361 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3421 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3481 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3541 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     3601 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3661 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     3721 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3781 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     3841 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     3901 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgact
     3961 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     4021 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4081 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4141 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4201 tgctggggat gcggtgggct ctatggctcg agttaattaa cgagagcata atattgatat
     4261 gtgccaaagt tgtttctgac tgactaataa gtataatttg tttctattat gtataggtta
     4321 agctaattac ttattttata atacaacatg actgttttta aagtacaaaa taagtttatt
     4381 tttgtaaaag agagaatgtt taaaagtttt gttactttat agaagaaatt ttgagttttt
     4441 gttttttttt aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat
     4501 gtaagtgtaa atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata
     4561 cagaccgata aaacacatgc gtcaatttta cgcatgatta tctttaacgt acgtcacaat
     4621 atgattatct ttctagggtt aaataatagt ttctaatttt tttattattc agcctgctgt
     4681 cgtgaatacc gagctccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     4741 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4801 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4861 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4921 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4981 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     5041 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5101 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5161 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     5221 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5281 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5341 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5401 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5461 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5521 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5581 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5641 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5701 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5761 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5821 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5881 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5941 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6001 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6061 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6121 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6181 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6241 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6301 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6361 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6421 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7141 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7201 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7261 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7321 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7381 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7441 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7501 ttacgccaag ctcgaaatta accctcacta aagggaacaa aagctggtac ctcgcgcgac
     7561 ttggtttgcc attctttagc gcgcgtcgcg tcacacagct tggccacaat gtggtttttg
     7621 tcaaacgaag attctatgac gtgtttaaag tttaggtcga gtaaagcgca aatctttt
//
