LOCUS       sequence-400926-        7676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of epithelial cell 
            SARS-CoV2 response..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7676)
  AUTHORS   Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, 
            Serresi M, Cicin-Sain L, Gargiulo G
  TITLE     Pharmacological modulators of epithelial immunity uncovered by 
            synthetic genetic tracing of SARS-CoV-2 infection responses.
  JOURNAL   Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. 
            Epub 2023 Jun 21.
  PUBMED    37343108
REFERENCE   2  (bases 1 to 7676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-23"; volume: "9(25):eadf4975. doi"; pages: " 
            10.1126/sciadv.adf4975. Epub 2023 Jun 21"
COMMENT     Sequence Label: pPB-Neo-COVGT1-d2EGFP
FEATURES             Location/Qualifiers
     source          1..7676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1092..1116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1117..1126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1123..1125,1126..1128,1129..1839,1840..1845,
                     1846..1968)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1168..1189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1429..1448)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1776..1797
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1969..1993)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2071..2090)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2136..2191
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2190..2209)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        2546..2925
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2926..3129
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3080..3100
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3162..3956
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3162..3956
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3216..3235)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3826..3845
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3994..4011)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4000..4224
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4574..4636
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4702..4721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4703..4721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4728..4745)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4728..4744)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4737..4759)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4886..5341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4973..4992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5183..5204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5367..5471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5472..5540,5541..6332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5690..5709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6503..7091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6992..7011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7245..7262
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7379..7400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7415..7420,7421..7438,7439..7445)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7453..7469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7458..7480
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7477..7493
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7477..7493
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7514..7532
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgtgga agcagccctg ggcacctggc
      361 atggacatgc ctgggcagac cccatgccaa atttcatcct gtctcatagc tttccattcc
      421 gtttcttttt cagtttctca ttgttacttc ctgccctgct ctggtttggt gagttttgtc
      481 cttcctttgg ataaaaacaa tgtccccaca cccagtgttg tgtaatcagg gaatgaaaga
      541 atctgctgtg atgccagcac tcaaggggac agtttctgtt gctgtgggaa cctgaacttt
      601 gcatttcctg actttggggt aattaaaatg tcagccttcc agatgctgac gcaacgaggt
      661 gtgggaggga gtcagggggc gtgtccagga ggttggccag gccaatcgga ggcggggcca
      721 gagggttgac tgggcaggcc gggggcgggg ctggaataat aataagagat ggtaggggcg
      781 ggtgtaggct ttgctttatg gtgatgccag gacagttttg ttttccattt ccctgtgcca
      841 taaaacggct ttcttggaaa gctaccagat attccccact aaaagtcttc aaggtcactc
      901 cgtggaaaag ggagggctac ttttgaaggg ggtcactttc cttcagctat gggggcagct
      961 gagccagagg tcaggctgta gtggggaggc cagaaggggc ggggacagcc gggattggga
     1021 ggggagaaca gcagcgtggg cggctggcag aggcagggaa agaccagcag agaggaaaga
     1081 gaaactggga gacaagtttg tacaaaaaag caggctgcca ccatggtgag caagggcgag
     1141 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1201 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1261 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1321 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1381 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1441 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1501 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1561 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1621 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1681 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1741 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1801 gccgccggga tcactctcgg catggacgag ctgtacaaga agcttagcca tggcttcccg
     1861 ccggaggtgg aggagcagga tgatggcacg ctgcccatgt cttgtgccca ggagagcggg
     1921 atggaccgtc accctgcagc ctgtgcttct gctaggatca atgtgtagac ccagctttct
     1981 tgtacaaagt ggtgatcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     2041 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     2101 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     2161 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     2221 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
     2281 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
     2341 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     2401 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     2461 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     2521 cgacctgcag cccaagcttc gcgttgacat tgattattga ctagttatta atagtaatca
     2581 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     2641 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     2701 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     2761 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     2821 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     2881 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     2941 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     3001 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     3061 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     3121 agcagagctc tctggctaac tagagaaccc actgcgccac catgattgaa caagatggat
     3181 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     3241 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     3301 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     3361 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     3421 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     3481 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     3541 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     3601 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     3661 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     3721 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     3781 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     3841 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     3901 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgactcg
     3961 agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     4021 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4081 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4141 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4201 ctggggatgc ggtgggctct atggctcgag ttaattaacg agagcataat attgatatgt
     4261 gccaaagttg tttctgactg actaataagt ataatttgtt tctattatgt ataggttaag
     4321 ctaattactt attttataat acaacatgac tgtttttaaa gtacaaaata agtttatttt
     4381 tgtaaaagag agaatgttta aaagttttgt tactttatag aagaaatttt gagtttttgt
     4441 ttttttttaa taaataaata aacataaata aattgtttgt tgaatttatt attagtatgt
     4501 aagtgtaaat ataataaaac ttaatatcta ttcaaattaa taaataaacc tcgatataca
     4561 gaccgataaa acacatgcgt caattttacg catgattatc tttaacgtac gtcacaatat
     4621 gattatcttt ctagggttaa ataatagttt ctaatttttt tattattcag cctgctgtcg
     4681 tgaataccga gctccaattc gccctatagt gagtcgtatt acaattcact ggccgtcgtt
     4741 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4801 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4861 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4921 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4981 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     5041 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     5101 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5161 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5221 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5281 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5341 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5401 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5461 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5521 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5581 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5641 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5701 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5761 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5821 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5881 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5941 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6001 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6061 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6121 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6181 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6241 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6301 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6361 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6421 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6481 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6541 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6601 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6661 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6721 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6781 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6841 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6901 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6961 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7021 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7081 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7141 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7201 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7261 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7321 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7381 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7441 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7501 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtacct cgcgcgactt
     7561 ggtttgccat tctttagcgc gcgtcgcgtc acacagcttg gccacaatgt ggtttttgtc
     7621 aaacgaagat tctatgacgt gtttaaagtt taggtcgagt aaagcgcaaa tctttt
//
