LOCUS       sequence-400932-       10378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10378)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1970
FEATURES             Location/Qualifiers
     source          1..10378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    6235..6823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6288..6308)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6706..6717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6895..7128
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7206..7327
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7243..7262)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7297..7316
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7367..7502
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7522..7541)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7523..7541)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7551..7568)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7551..7567)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7560..7582)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7709..8164
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7796..7815)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8006..8027
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8190..8294
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8295..8363,8364..9155)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8513..8532)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9326..9914
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9815..9834
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10068..10085
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10202..10223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10238..10243,10244..10261,10262..10268)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10276..10292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10281..10303
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10300..10316
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10300..10316
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10335..10355
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10337..10355
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgacc aagtttcagg agatggtgac
     4141 attcaaggat gtggctgtgg tcttcaccag ggaggagctg gggttgctgg accttgccca
     4201 gagaaagctg taccaagatg tgatgctgga gaacttcagg aacctgctgt cagtgggcta
     4261 tcaacccttc aaactagatg tgatattaca gttgggaaaa gaagacaagc ttcggatgat
     4321 ggagacagaa atccaaggag atgggtgttc aggacacaag aatcaaaatg agatagatac
     4381 ccttcaagaa gtaagattaa gattcctttc atatgaagac cttatatgct ggcaaatatg
     4441 ggaacaattt acaagtaaat taaccagtaa tcaagaccta ataataaatc ttcaaggcaa
     4501 gaggtccaag ttgctaaaac aaggtgattc cccctgtcag gtgtggacag gagaatctag
     4561 tcaggtctct gaagatgaga actatgtaat aaagctacaa ggggagagtt caaatagcat
     4621 aaaaaatcaa gagcttccat tgaggaccac ctgggatttc tggaggaaaa tgtatctgag
     4681 agaaccacag aattatcaga gtaggtgtca gcaaattgat gtaaaaaata agctctgtaa
     4741 atgtgatcat tgtgttaggc aaagaattgc tcatcaacat gatgatcatg gagtacacaa
     4801 aagagagaaa gcttttagcc acaataattg tggaaaagac tgtgtgaagg aatcatccca
     4861 gcatagcata atccaatcag gagagcaaac ctctgatgaa aatggaaaag gcttaagtgt
     4921 tggctctaat cttgaacttc accagcaact acacttaaga gacaagcctc atgtaaatgt
     4981 tgagtacggg aagggcatag gttacagctc agggcttccc aggcatcagt gtttccacat
     5041 aggagagaaa tgctatagga atggtgacag tggtgagggc ttcagtcagg gctcacatct
     5101 gcaacctcat cagagagtca gcacaggaga gaacctctac agatgtcagg tatatgcccg
     5161 gagctccaac cagaactcct gtcttccctc tcatgagctt actcacccag gagagaagtt
     5221 gtgtacatgt ggcaggtgtg ggaagggctt ccatcatagc ttagattttg acattcactg
     5281 tgtagacagt gctggagaga gagcctgtaa atgtgatgta tatgataaag gcttcagtca
     5341 gacatcacaa cttcaagccc atcagagagg tcactctaga gacaagacat acaaatggga
     5401 agtaagtgac aggatattta ataggaattc tggtcttcac cagagagttc acactggaga
     5461 gaaaccatat aaatgtgagg tatgtgataa gggcttcagt aaggcctcaa atcttcaagc
     5521 ccatcagaga atccacactg gagagaaacc ctacaaatgt gatgtgtgtg ataagaactt
     5581 cagccgtaat tcccaccttc aggcccatca gagagtccat acaggagaga aaccctacaa
     5641 atgtgacaca tgtgggaagg acttcagtca gatctctcat cttcaggccc atcagagagt
     5701 tcacaaagga gagaagccat acaaatgtga gacatgtggg aagggcttta gtcagagttc
     5761 gcatctccaa gaccatcagc aagtccatac tggagagaat ccctacaaat gtgatgtgtg
     5821 tgggaaaggc ttcagttgga gttcacatct tcaagcccat cagagagtcc acacaggaga
     5881 gaaaccatac aaatgtgaag aatgtaggaa aggcttcatc tggaactcat atcttcatgt
     5941 tcatcagagg atccacacgg gagagaaacc ctataaatgt ggcatgtgtg gtaagagctt
     6001 cagtcagact tcacatcttc aagcccatca gagagtccat actggagaga aaccatacaa
     6061 atgttttgtg tgtggtaagg gctttagtaa gagttcgttg tcttcagatt catcagagag
     6121 tccatagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     6181 ttgtggaaag gacgataaca tctgccatcc cacgcgagca cgcgttaagt cgacaatcaa
     6241 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6301 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6361 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     6421 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     6481 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     6541 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     6601 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     6661 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     6721 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     6781 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     6841 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6901 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6961 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7021 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7081 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     7141 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7201 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7261 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7321 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     7381 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7441 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7501 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     7561 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     7621 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     7681 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     7741 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7801 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7861 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7921 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7981 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8041 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     8101 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     8161 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     8221 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     8281 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     8341 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     8401 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     8461 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     8521 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     8581 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     8641 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     8701 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     8761 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     8821 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     8881 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     8941 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     9001 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     9061 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     9121 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     9181 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     9241 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     9301 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     9361 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     9421 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     9481 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     9541 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     9601 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     9661 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     9721 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     9781 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     9841 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcagggtggc
     9901 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     9961 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    10021 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    10081 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    10141 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    10201 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    10261 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    10321 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
