LOCUS       sequence-400934-       12847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12847)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2528
FEATURES             Location/Qualifiers
     source          1..12847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8704..9292
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8757..8777)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9175..9186)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9364..9597
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9675..9796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9712..9731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9766..9785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9836..9971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9991..10010)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9992..10010)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10020..10037)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10020..10036)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10029..10051)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10178..10633
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10265..10284)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10475..10496
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10659..10763
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10764..10832,10833..11624)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10982..11001)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11795..12383
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12284..12303
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12537..12554
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12671..12692
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12707..12712,12713..12730,12731..12737)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12745..12761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12750..12772
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12769..12785
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12769..12785
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12804..12824
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12806..12824
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcca aatgagggaa tcccccactc
     4141 aagtcaaact caggagcaag actgtttaca gagtcaacct gtcagtaata atgaagaaat
     4201 ggcaatcaag caagaaagtg gtggtgatgg ggaggtggag gagtacctct cctttcgttc
     4261 tgtgggtgac gggctgtcca cctctgctgt ggggtgcgca tcagcagctc cgaggagagg
     4321 gccagccctg ctgcacatcg accgacatca gatccaggca gtagagccta gcgcccaggc
     4381 ccttgagctg cagggtttgg gtgtggacgt ctatgaccag gacgtgctgg aacagggagt
     4441 gcttcagcag gtggacaatg ccatccatga ggccagccgt gcctcccagc tcgttgacgt
     4501 ggagaaggag tatcggtcgg tcctggatga cctcacgtca tgtacgacat ccctaaggca
     4561 aatcaataaa attattgaac agcttagccc tcaagctgcc accagcagag acatcaacag
     4621 gaaactagat tctgtaaaac gacagaagta taataaggaa caacagctaa aaaagatcac
     4681 tgcaaaacaa aagcatctcc aggccatcct tggaggagca gaggtgaaaa ttgaactaga
     4741 tcacgccagt ctggaggagg atgcagagcc ggggccatcc agtcttggca gcatgctcat
     4801 gcctgtccag gagactgcct gggaagagct catccgcact ggccagatga caccttttgg
     4861 tacccagatc cctcagaaac aggagaaaaa gcccagaaaa atcatgctta atgaagcatc
     4921 aggcttcgaa aagtatttgg cagatcaagc aaaactgtct tttgaaagga agaagcaagg
     4981 ttgtaataaa agagcagcta gaaaagctcc agccccagtc acgcctccag ccccagtgca
     5041 aaataaaaac aaaccaaaca agaaagccag agttctgtcc aaaaaagagg agcgtttgaa
     5101 aaagcacatc aagaaactcc agaagagggc tttgcagttc caggggaaag tgggattgcc
     5161 aaaggcaagg agaccttggg agtcagacat gaggccagag gcagagggag actctgaggg
     5221 tgaagagtct gagtatttcc ccacagagga ggaggaagag gaggaagatg acgaggtgga
     5281 gggggcagag gcggacctgt ctggagatgg tactgactat gagctgaagc ctctgcccaa
     5341 gggcgggaaa cggcagaaga aagtgccagt gcaggagatt gatgatgact ttttcccaag
     5401 ttctggggaa gaagctgaag ctgcttctgt aggagaagga ggaggaggag gtcggaaagt
     5461 gggaagatac cgagatgatg gagatgaaga ttattataag cagcggttaa ggagatggaa
     5521 taaactgaga ctgcaggaca aagagaaacg tctgaagctg gaggacgatt ctgaggaaag
     5581 tgatgctgaa tttgacgaag gttttaaagt gccaggtttt ctgttcaaaa agctttttaa
     5641 gtaccagcag acaggtgtta ggtggctgtg ggaattgcac tgccagcagg caggaggaat
     5701 tctgggagat gaaatgggat tgggcaagac catccagata attgccttct tggcaggtct
     5761 gagctacagc aagatcagga ctcgtggttc aaattacagg tttgaggggt tgggtccaac
     5821 tgtaattgtc tgtccaacaa cagtgatgca tcagtgggtg aaggaatttc acacgtggtg
     5881 gcctccgttc agagtggcaa ttctacatga aaccggttcc tatacccaca aaaaggagaa
     5941 actaattcga gatgttgctc attgtcatgg aattttgatc acatcttact cctacattcg
     6001 attgatgcag gatgacatta gcaggtatga ctggcactat gtgatcttgg acgaaggaca
     6061 caaaattcga aatccaaatg ctgctgtcac ccttgcttgc aaacagtttc gcacccctca
     6121 tcggatcatt ctgtctggct caccgatgca aaataacctc cgagagctgt ggtcgctctt
     6181 tgacttcatc ttcccgggaa agttaggcac gttgcctgtg tttatggagc agttctccgt
     6241 ccccatcacc atggggggat attcaaatgc ttccccagta caggtcaaaa ctgcttacaa
     6301 gtgtgcatgt gtcttacgag ataccataaa tccataccta ctgcggagaa tgaagtcaga
     6361 tgtcaagatg agcctttctt tgccagataa aaatgaacag gtcttatttt gccgtcttac
     6421 agatgagcag cataaagtct accaaaattt cgttgattcc aaagaagttt acaggattct
     6481 caatggagag atgcagattt tctccggact tatagcccta agaaaaattt gcaaccaccc
     6541 tgatctcttt tctggaggtc ccaagaatct caaaggtctt cctgatgatg aactagaaga
     6601 agatcagttt gggtactgga aacgttctgg gaaaatgatt gttgttgagt ctttgttgaa
     6661 aatatggcac aagcagggtc agcgagtatt gctgttttct cagtcaaggc agatgctgga
     6721 catacttgaa gtattcctta gagcccaaaa gtatacctat ctcaagatgg atggtaccac
     6781 tacaatagct tcaagacagc cactgattac gagatacaat gaggacacat ccatatttgt
     6841 gtttcttctg accacgcggg tgggcggctt aggtgtcaac ctgacggggg caaacagagt
     6901 tgtcatctat gacccagact ggaacccaag cacggacacg caggcccggg agcgagcatg
     6961 gagaataggc cagaagaagc aagtgactgt gtacaggctc ctgactgcgg gcaccattga
     7021 agaaaagatc taccaccgac aaatcttcaa gcagtttttg acaaatagag tgctaaaaga
     7081 cccaaaacaa aggcggtttt tcaaatccaa tgatctctat gagctattta ctctgactag
     7141 tcctgatgca tcccagagca ctgaaacaag tgcaattttt gcaggaactg gatcagatgt
     7201 tcagacaccc aaatgccatc taaaaagaag gattcaacca gcctttggag cagaccatga
     7261 tgttccaaaa cgcaagaagt tccctgcttc taacatatct gtaaatgatg ccacatcatc
     7321 tgaagagaaa tctgaggcta aaggagctga agtaaatgca gtaacttcta atcgaagtga
     7381 tcctttgaaa gatgaccctc acatgagtag taatgtaact agcaatgata ggcttggaga
     7441 agagacaaat gcagtatctg gaccagaaga gttgtcagtg attagtggaa atggggaatg
     7501 ttcaaattct tcaggaacag gcaaaacttc tatgccatct ggtgatgaaa gcattgatga
     7561 aaagttaggt ctttcttaca aaagagaaag acccagccag gctcaaacag aagctttttg
     7621 ggagaataaa caaatggaaa ataattttta taagcacaag tcaaaaacaa aacatcatag
     7681 tgtggcagaa gaagagaccc tggagaaaca tctgagacca aagcaaaagc ctaagaactc
     7741 taagcattgc agagacgcca agtttgaagg aactcgaatt ccacacctgg tgaagaaaag
     7801 gcgttaccag aagcaagaca gtgaaaacaa gagtgaggcc aaggaacaga gcaatgacga
     7861 ttatgttttg gaaaagcttt tcaaaaaatc agttggcgtg cacagtgtca tgaagcacga
     7921 tgccatcatg gatggagcca gcccagatta tgtactggtg gaggcagaag ccaaccgagt
     7981 ggcccaggat gccctgaaag cactgaggct ctctcgtcag cggtgtctgg gagcagtgtc
     8041 tggtgttccc acctggactg gccacagggg gatttctggt gcaccagcag gaaaaaagag
     8101 tagatttggt aagaaaagga attctaactt ctctgtgcag catccttcat caacatctcc
     8161 aacagagaag tgccaggatg gcatcatgaa aaaggaggga aaagataatg tccctgagca
     8221 ttttagtgga agagcagaag atgcagactc ttcatccggg cccctcgctt cctcctcact
     8281 cttggctaaa atgagagcta gaaaccacct gattctgcca gagcgtttag aaagtgaaag
     8341 cgggcacctg caggaagctt ctgccctgct gcccaccaca gaacacgatg accttctggt
     8401 ggagatgaga aacttcatcg ctttccaggc ccacactgat ggccaggcca gcaccaggga
     8461 gatactgcag gagtttgaat ccaagttatc tgcatcacag tcttgtgtct tccgagaact
     8521 attgagaaat ctgtgcactt tccatagaac ttctggtggt gaaggaattt ggaaactcaa
     8581 gccagaatac tgctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     8641 ttatatatct tgtggaaagg acgaacgcaa tttacggcct ggttgtggac gcgttaagtc
     8701 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     8761 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     8821 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     8881 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     8941 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     9001 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     9061 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     9121 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     9181 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     9241 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     9301 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     9361 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     9421 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9481 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9541 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     9601 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     9661 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9721 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9781 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     9841 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     9901 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     9961 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
    10021 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
    10081 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
    10141 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
    10201 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
    10261 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
    10321 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
    10381 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
    10441 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
    10501 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
    10561 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
    10621 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10681 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10741 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    10801 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    10861 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10921 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    10981 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    11041 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    11101 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    11161 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    11221 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    11281 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    11341 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    11401 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    11461 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    11521 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    11581 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    11641 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11701 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11761 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    11821 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    11881 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    11941 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    12001 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    12061 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    12121 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    12181 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    12241 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    12301 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    12361 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    12421 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    12481 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    12541 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    12601 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    12661 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    12721 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    12781 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    12841 tgcaagc
//
