LOCUS       sequence-400945-        7675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of epithelial cell 
            SARS-CoV2 response..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7675)
  AUTHORS   Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, 
            Serresi M, Cicin-Sain L, Gargiulo G
  TITLE     Pharmacological modulators of epithelial immunity uncovered by 
            synthetic genetic tracing of SARS-CoV-2 infection responses.
  JOURNAL   Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. 
            Epub 2023 Jun 21.
  PUBMED    37343108
REFERENCE   2  (bases 1 to 7675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-23"; volume: "9(25):eadf4975. doi"; pages: " 
            10.1126/sciadv.adf4975. Epub 2023 Jun 21"
COMMENT     Sequence Label: pPB-Neo-COVGT2-d2EGFP
FEATURES             Location/Qualifiers
     source          1..7675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1091..1115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1116..1125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1122..1124,1125..1127,1128..1838,1839..1844,
                     1845..1967)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1167..1188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1428..1447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1775..1796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(1968..1992)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2070..2089)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2135..2190
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2189..2208)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        2545..2924
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2925..3128
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3079..3099
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3161..3955
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3161..3955
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3215..3234)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3825..3844
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3993..4010)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3999..4223
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4573..4635
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4701..4720)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4702..4720)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4727..4744)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4727..4743)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4736..4758)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4885..5340
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4972..4991)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5182..5203
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5366..5470
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5471..5539,5540..6331)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5689..5708)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6502..7090
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6991..7010
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7244..7261
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7378..7399
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7414..7419,7420..7437,7438..7444)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7452..7468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7457..7479
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7476..7492
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7476..7492
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        7513..7531
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgtcag cgctcctgac tcactggccg
      361 cctacccacc atatcatcct tcgaaactcg gatccccacg tttttgggga gaaggatttg
      421 agtaataata aaactcccgt ctcccccgca gccggctctg cgtgaattac actttcctta
      481 ttgcgatagt caggatatct tgctgactgg gtcacccttc ttcacaggac acacacacag
      541 ggccatgaaa acggccttct tcaaagatcc cacggctcgg gcctcccttc cggggccacc
      601 ttgcccagtg gccgggggct ttccttccca gaagcctgag gccttattcc tcccttcccc
      661 ccaaaactga cacctttgtt agccacctcc ccacccacat acatttctgc cagtgttcac
      721 aatgacactc agcggtcatg tctggacatg agtgcccagg gaatatgccc aagctatgcc
      781 ttgtcctctg ggaaaaatga aagtgaccta ggacacctgc cttcatcact gtatgcacaa
      841 cagcagcact gaaaactcac cccacagact gctgcaccct cacagagcag cgtagtcttg
      901 acttatgcaa tgtggccttt ggacacatct tctgaatcag ccccttccat ggcagggctg
      961 accccgcctc ctccatccga ccccgcctcc tccagccctc cccgccccac tacccaggaa
     1021 ggcggagctg ggtgcgagcg ccctaccgct ttcgctttcc cttcgcggtg cccactccac
     1081 tccttgtgcg gcaagtttgt acaaaaaagc aggctgccac catggtgagc aagggcgagg
     1141 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1201 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1261 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1321 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1381 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1441 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1501 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1561 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1621 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1681 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1741 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1801 ccgccgggat cactctcggc atggacgagc tgtacaagaa gcttagccat ggcttcccgc
     1861 cggaggtgga ggagcaggat gatggcacgc tgcccatgtc ttgtgcccag gagagcggga
     1921 tggaccgtca ccctgcagcc tgtgcttctg ctaggatcaa tgtgtagacc cagctttctt
     1981 gtacaaagtg gtgatcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     2041 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     2101 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     2161 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     2221 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     2281 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     2341 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     2401 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     2461 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     2521 gacctgcagc ccaagcttcg cgttgacatt gattattgac tagttattaa tagtaatcaa
     2581 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     2641 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2701 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2761 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2821 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2881 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2941 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     3001 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     3061 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     3121 gcagagctct ctggctaact agagaaccca ctgcgccacc atgattgaac aagatggatt
     3181 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     3241 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     3301 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     3361 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     3421 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     3481 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     3541 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     3601 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     3661 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     3721 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     3781 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3841 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     3901 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgactcga
     3961 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     4021 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4081 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4141 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4201 tggggatgcg gtgggctcta tggctcgagt taattaacga gagcataata ttgatatgtg
     4261 ccaaagttgt ttctgactga ctaataagta taatttgttt ctattatgta taggttaagc
     4321 taattactta ttttataata caacatgact gtttttaaag tacaaaataa gtttattttt
     4381 gtaaaagaga gaatgtttaa aagttttgtt actttataga agaaattttg agtttttgtt
     4441 tttttttaat aaataaataa acataaataa attgtttgtt gaatttatta ttagtatgta
     4501 agtgtaaata taataaaact taatatctat tcaaattaat aaataaacct cgatatacag
     4561 accgataaaa cacatgcgtc aattttacgc atgattatct ttaacgtacg tcacaatatg
     4621 attatctttc tagggttaaa taatagtttc taattttttt attattcagc ctgctgtcgt
     4681 gaataccgag ctccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt
     4741 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     4801 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     4861 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4921 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4981 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5041 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5101 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5161 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5221 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5281 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5341 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5401 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5461 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5521 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5581 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5641 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5701 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5761 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5821 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5881 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5941 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6001 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6061 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6121 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6181 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6241 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6301 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6361 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6421 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6481 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6541 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6601 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6661 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6721 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6781 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6841 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6901 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6961 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7021 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7081 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7141 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     7201 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     7261 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     7321 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     7381 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     7441 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     7501 cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtacctc gcgcgacttg
     7561 gtttgccatt ctttagcgcg cgtcgcgtca cacagcttgg ccacaatgtg gtttttgtca
     7621 aacgaagatt ctatgacgtg tttaaagttt aggtcgagta aagcgcaaat ctttt
//
