LOCUS       sequence-400986-        4260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC43A2_STOP
FEATURES             Location/Qualifiers
     source          1..4260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1817..1916)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1933..1952)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1934..1952)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1957..1973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1957..1973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2075..2094)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2086..2895
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2153..2172)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3042..3630
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3531..3550
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3784..3801
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3960..3987)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4079..4165)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4214..4236
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4228..4245
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4229..4245
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc acccaccctg
      121 gcaacagcac accggagaag gtggtggatg gcatgcaccg ccgtgctgga gaacctgctg
      181 ttctctgccg tgctgctggg atggggaagc ctgctgatca tgctgaagag cgagggcttt
      241 tactcctatc tgtgcaccga gccagagaac gtgaccaatg gaacagtggg aggaacagca
      301 gagccaggac acgaggaggt gtcctggatg aacggctggc tgtcttgtca ggcccaggac
      361 gagatgctga atctggcctt caccgtgggc tcctttctgc tgtctgccat cacactgccc
      421 ctgggcatcg tgatggataa gtacggccct agaaagctga ggctgctggg atccgcctgc
      481 ttcgccgtgt cttgtctgct gatcgcctat ggcgccagca agcctaacgc cctgtccgtg
      541 ctgatcttta tcgccctggc cctgaatggc ttcggcggca tgtgcatgac ctttacaagc
      601 ctgaccctgc caaatatgtt cggcgacctg cggagcacct tcatcgccct gatgatcggc
      661 tcttacgcaa gctccgccgt gaccttccca ggcatcaagc tgatctatga tgccggcgtg
      721 tcttttatcg tggtgctggt ggtgtgggca ggatgcagcg gcctggtgtt cctgaactgt
      781 ttctttaatt ggccactgga gccctttcct ggcccagagg acatggatta ctctgtgaag
      841 atcaagttca gctggctggg ctttgaccac aagatcaccg gcaagcagtt ctataagcag
      901 gtgaccacag tgggccgccg gctgagcgtg ggctctagca tgcggtccgc caaggagcag
      961 gtggccctgc aggagggcca caagctgtgc ctgagcacag tggacctgga ggtgaagtgt
     1021 cagcctgatg ccgccgtggc cccatccttc atgcacagcg tgttttcccc catcctgctg
     1081 ctgtctctgg tgaccatgtg cgtgacacag ctgcgcctga tcttctacat gggcgccatg
     1141 aacaatatcc tgaagtttct ggtgagcggc gatcagaaga ccgtgggcct gtacacatcc
     1201 atcttcggcg tgctgcagct gctgtgcctg ctgaccgcac ctgtgatcgg ctatatcatg
     1261 gactggcggc tgaaggagtg tgaggatgcc tccgaggagc cagaggagaa ggacgccaac
     1321 cagggcgaga agaagaagaa gaagcgggat cggcagatcc agaagatcac caacgccatg
     1381 agagccttcg cctttacaaa tctgctgctg gtgggcttcg gcgtgacctg tctgatcccc
     1441 aatctgcctc tgcagatcct gagcttcatc ctgcacacaa tcgtgagggg ctttatccac
     1501 tccgccgtgg gaggactgta cgcagccgtg tatccatcta cccagtttgg cagcctgaca
     1561 ggcctgcagt ctctgatcag cgccctgttc gcactgctgc agcagcctct gtttctggca
     1621 atgatgggac cactgcaggg cgacccactg tgggtgaacg tgggactgct gctgctgtcc
     1681 ctgctgggct tctgcctgcc tctgtacctg atctgttata gaaggcagct ggagaggcag
     1741 ctgcagcaga ggcaggagga cgataagctg ttcctgaaga tcaacggctc ctctaatcag
     1801 gaggcctttg tgtaagaccc agctttcttg tacaaagttg gcattataag aaagcattgc
     1861 ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat
     1921 ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc ctggcagctc
     1981 tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga
     2041 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
     2101 cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa
     2161 tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc
     2221 gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat
     2281 gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt
     2341 atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc
     2401 caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc
     2461 ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt
     2521 cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat
     2581 gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca
     2641 ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac
     2701 gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag
     2761 gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt
     2821 tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc
     2881 gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg
     2941 acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc
     3001 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     3061 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     3121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     3181 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     3241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     3301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     3361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     3421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     3481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     3541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3661 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3721 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3781 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3841 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     3901 agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa
     3961 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     4021 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     4081 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     4141 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta
     4201 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
//
