LOCUS       sequence-400987-        4029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF716 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF716
FEATURES             Location/Qualifiers
     source          1..4029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1586..1685)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1702..1721)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1703..1721)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1726..1742)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1726..1742)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1844..1863)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1855..2664
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1922..1941)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2811..3399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3300..3319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3553..3570
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3729..3756)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3848..3934)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3983..4005
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3997..4014
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3998..4014
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tggccaagag gcctggccca
      121 cctggcagca gagagatggg cctgctgacc ttcagagaca tcgccatcga gtttagcctg
      181 gccgagtggc agtgtctgga ccacgcccag cagaacctgt acagagacgt gatgctggag
      241 aactacagaa acctggtgag cctgggcatc gccgtgagca agcccgacct gattacctgc
      301 ctggagcaga acaaggagcc ccagaacatc aagagaaacg agatggtggc caagcacccc
      361 gtgacctgca gccacttcac ccaggatctg cagagcgagc agggcatcaa ggacagcctg
      421 cagaaggtga tcctgagaag atacggcaag tgcggccagg aggacctcca ggtgaagaag
      481 tgctgcaaga gcgtgggcga gtgcgaggtg cacaagggcg gctacaacta cgtgaaccag
      541 tgcctgagcg ccacccagaa taagaccttt cagacacaca agtgcgtgaa ggtgttcggc
      601 aagttcagca actccaacag acacaagaca aggcacacag gcaagaagca tttcaagtgt
      661 aagaacgacg gcaagagctt ctgcatgctg agcagactga accagcacca gatcattcac
      721 accagagaga agagctataa gtgcgaagag tgcgggaaga gctttaactg cagctctacc
      781 ctgaccaggc acaaaagaat tcacactggc gaaaagcctt acaggtgtga agagtgtgga
      841 aaagcattct cctggagcgc cagcctgacc aagcacaagc ggatccatac aggcgagaaa
      901 ccctatacct gcgaggagag gggcaaggtg tttagcagaa gcaccctcac caactacaag
      961 cgcattcata ccggcgaaaa accctacact tgcgaggaat gtggtaaggc cttcagcagg
     1021 agctccaccc tgacaaatca caagaggatc cacacaggag agaggcccta caaatgcgaa
     1081 gaatgcggaa aggctttctc cctgagcagc accctgaaga agcacaaaat cgtgcacacc
     1141 ggagagaaac tgtacacctg tgaggaatgc ggcaaggcct ttacctttag cagcacactg
     1201 aacacccaca agagaatcca caccggtgag aagccctaca catgtgaaga atgtggcaag
     1261 gcattcagcc tgccctctac cttcacctac cataagagaa cacacaccgg cgagaagcct
     1321 tataaatgcg aggagtgcgg caaagccttc aactgctcct ccactctgaa gaaacacaag
     1381 atcatccaca ctggagagaa gctgtacaag tgcaaggagt gtggcaaagc tttcaccttc
     1441 agctcaaccc tgaacactca caagcgaatc cataccgggg agaagccata caagtgtgag
     1501 gagtgtgacc agaccttcaa gtggcacagc tccctggcca accacaagaa catgcacact
     1561 ggggagaaac cttacaagta cgagtaccca actttcttgt acaaagttgg cattataaga
     1621 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1681 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     1741 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1801 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc
     1861 catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat
     1921 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat
     1981 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2041 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2101 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa
     2161 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2221 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2281 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     2341 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa
     2401 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     2461 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     2521 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     2581 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     2641 ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc
     2701 attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac
     2761 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2821 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2881 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2941 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     3001 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3061 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     3121 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3181 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3241 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3301 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3361 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3421 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3481 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3541 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     3601 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     3661 aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta
     3721 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt
     3781 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc
     3841 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
     3901 cccagtcttc cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg
     3961 ttaacgctag catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag
     4021 ctcgggccc
//
