LOCUS       sequence-400988-        4413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC6A6_STOP
FEATURES             Location/Qualifiers
     source          1..4413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1970..2069)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2086..2105)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2087..2105)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2110..2126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2110..2126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2228..2247)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2239..3048
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2306..2325)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3195..3783
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3684..3703
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3937..3954
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4113..4140)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4232..4318)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4367..4389
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4381..4398
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4382..4398
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc caccaaggag
      121 aagctgcagt gcctgaagga cttccacaag gatatcctga agccaagccc tggcaagtcc
      181 cctggaacaa ggccagagga cgaggcagag ggcaagccac ctcagagaga gaagtggagc
      241 tccaagatcg attttgtgct gtccgtggca ggaggattcg tgggactggg caacgtgtgg
      301 cggtttccct acctgtgcta taagaatggc ggcggcgcct tcctgatccc atacttcatc
      361 tttctgttcg gcagcggcct gcccgtgttc tttctggaga tcatcatcgg ccagtatacc
      421 agcgagggcg gcatcacatg ctgggagaag atctgtcctc tgttctccgg catcggctac
      481 gcctctgtgg tcatcgtgag cctgctgaac gtgtactata tcgtgatcct ggcctgggcc
      541 acctactatc tgtttcagtc tttccagaag gagctgcctt gggcccactg caaccacagc
      601 tggaataccc cacactgtat ggaggacaca atgcggaaga acaagagcgt gtggatcacc
      661 atctctagca ccaacttcac ctcccccgtg atcgagttct gggagagaaa tgtgctgagc
      721 ctgtccccag gaatcgacca ccctggctct ctgaagtggg atctggccct gtgcctgctg
      781 ctggtgtggc tggtgtgctt cttttgtatc tggaagggcg tgcggagcac cggcaaggtg
      841 gtgtacttta ccgccacatt tcctttcgcc atgctgctgg tgctgctggt gaggggactg
      901 acactgccag gagcaggagc aggcatcaag ttctacctgt atccagatat caccaggctg
      961 gaggacccac aagtgtggat cgatgccggc acacagatct tctttagcta cgccatctgt
     1021 ctgggcgcca tgacctccct gggctcttac aacaagtaca agtacaactc ctacagggac
     1081 tgcatgctgc tgggctgtct gaacagcggc accagcttcg tgagcggctt tgccatcttc
     1141 tccatcctgg gcttcatggc acaggagcag ggagtggaca tcgcagatgt ggcagagtct
     1201 ggacctggac tggcctttat cgcctaccca aaggccgtga ccatgatgcc tctgccaaca
     1261 ttctggtcca tcctgttctt tatcatgctg ctgctgctgg gcctggactc tcagtttgtg
     1321 gaggtggagg gccagatcac ctccctggtg gatctgtacc catctttcct gaggaagggc
     1381 tatcggagag agatctttat cgccttcgtg tgctctatca gctacctgct gggcctgacc
     1441 atggtgacag agggcggcat gtacgtgttc cagctgttcg attactatgc agcatccggc
     1501 gtgtgcctgc tgtgggtggc cttctttgag tgttttgtga tcgcctggat ctacggcggc
     1561 gacaacctgt atgatggcat cgaggacatg atcggataca ggccaggacc ttggatgaag
     1621 tattcttggg ccgtgatcac ccctgtgctg tgcgtgggct gttttatctt cagcctggtg
     1681 aagtacgtgc ccctgaccta taacaagaca tacgtgtatc ctaattgggc aatcggactg
     1741 ggatggtccc tggccctgtc ctctatgctg tgcgtgcccc tggtcatcgt gatcaggctg
     1801 tgccagaccg agggaccttt cctggtgcgc gtgaagtacc tgctgacacc aagggagccc
     1861 aatagatggg cagtggagag ggagggagca accccatata acagcagaac agtgatgaat
     1921 ggcgccctgg tgaagcccac ccacatcatc gtggagacaa tgatgtaaga cccagctttc
     1981 ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc
     2041 actatcagtc aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg
     2101 tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt
     2161 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
     2221 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
     2281 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
     2341 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
     2401 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
     2461 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
     2521 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     2581 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     2641 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     2701 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     2761 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     2821 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     2881 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     2941 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     3001 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     3061 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     3121 ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag
     3181 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3241 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3301 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3361 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3421 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3481 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3541 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3601 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3661 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3721 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3781 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3841 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3901 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3961 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4021 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt
     4081 accgctagcc aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct
     4141 gcttagtttg atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt
     4201 gcttcacaac gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg
     4261 acaaacaaca gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat
     4321 gcctggcagt tccctactct cgcgttaacg ctagcatgga tgttttccca gtcacgacgt
     4381 tgtaaaacga cggccagtct taagctcggg ccc
//
