LOCUS       sequence-401021-        8645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of epithelial cell 
            SARS-CoV2 response..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, 
            Serresi M, Cicin-Sain L, Gargiulo G
  TITLE     Pharmacological modulators of epithelial immunity uncovered by 
            synthetic genetic tracing of SARS-CoV-2 infection responses.
  JOURNAL   Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. 
            Epub 2023 Jun 21.
  PUBMED    37343108
REFERENCE   2  (bases 1 to 8645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-23"; volume: "9(25):eadf4975. doi"; pages: " 
            10.1126/sciadv.adf4975. Epub 2023 Jun 21"
COMMENT     Sequence Label: pPB-Neo-COVGT2-d2EGFP-mCherry
FEATURES             Location/Qualifiers
     source          1..8645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1091..1115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1116..1125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1122..1124,1125..1127,1128..1838,1839..1844,
                     1845..1967)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1167..1188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1428..1447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1775..1796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1971..1980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2122..2140)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             2703..2723
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2727..2747
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2751..2771
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2910..2934)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2938..2962)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3040..3059)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3105..3160
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3159..3178)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        3515..3894
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3895..4098
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4049..4069
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4131..4925
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4131..4925
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4185..4204)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4795..4814
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4963..4980)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4969..5193
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5543..5605
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5671..5690)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5672..5690)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5697..5714)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5697..5713)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5706..5728)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5855..6310
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5942..5961)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6152..6173
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6336..6440
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6441..6509,6510..7301)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6659..6678)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7472..8060
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7961..7980
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8214..8231
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8348..8369
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8384..8389,8390..8407,8408..8414)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8422..8438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8427..8449
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8446..8462
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8446..8462
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8483..8501
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgtcag cgctcctgac tcactggccg
      361 cctacccacc atatcatcct tcgaaactcg gatccccacg tttttgggga gaaggatttg
      421 agtaataata aaactcccgt ctcccccgca gccggctctg cgtgaattac actttcctta
      481 ttgcgatagt caggatatct tgctgactgg gtcacccttc ttcacaggac acacacacag
      541 ggccatgaaa acggccttct tcaaagatcc cacggctcgg gcctcccttc cggggccacc
      601 ttgcccagtg gccgggggct ttccttccca gaagcctgag gccttattcc tcccttcccc
      661 ccaaaactga cacctttgtt agccacctcc ccacccacat acatttctgc cagtgttcac
      721 aatgacactc agcggtcatg tctggacatg agtgcccagg gaatatgccc aagctatgcc
      781 ttgtcctctg ggaaaaatga aagtgaccta ggacacctgc cttcatcact gtatgcacaa
      841 cagcagcact gaaaactcac cccacagact gctgcaccct cacagagcag cgtagtcttg
      901 acttatgcaa tgtggccttt ggacacatct tctgaatcag ccccttccat ggcagggctg
      961 accccgcctc ctccatccga ccccgcctcc tccagccctc cccgccccac tacccaggaa
     1021 ggcggagctg ggtgcgagcg ccctaccgct ttcgctttcc cttcgcggtg cccactccac
     1081 tccttgtgcg gcaagtttgt acaaaaaagc aggctgccac catggtgagc aagggcgagg
     1141 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1201 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1261 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1321 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1381 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1441 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1501 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1561 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1621 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1681 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1741 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1801 ccgccgggat cactctcggc atggacgagc tgtacaagaa gcttagccat ggcttcccgc
     1861 cggaggtgga ggagcaggat gatggcacgc tgcccatgtc ttgtgcccag gagagcggga
     1921 tggaccgtca ccctgcagcc tgtgcttctg ctaggatcaa tgtgtagact gccaccatgg
     1981 tgagcaaggg cgaggaggac aacatggcca tcatcaagga gttcatgagg ttcaaggtgc
     2041 acatggaggg cagcgtgaac ggccacgagt tcgagatcga gggcgagggc gagggcaggc
     2101 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     2161 cctgggacat cctgagcccc cagttcatgt acggcagcaa ggcctacgtg aagcaccccg
     2221 ccgacatccc cgactacctg aagctgagct tccccgaggg cttcaactgg gagagggtga
     2281 tgaacttcga ggacggcggc gtggtgaccg tgacccagga cagcagcctg caggacggcg
     2341 agttcatcta caaggtgaag ctgaggggca ccaacttccc cagcgacggc cccgtgatgc
     2401 agtgcaggac catgggctgg gaggccagca ccgagaggat gtaccccgag gacggcgccc
     2461 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgccgagg
     2521 tgaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtggaca
     2581 tcaagctgga catcctgagc cacaacgagg actacaccat cgtggagcag tacgagaggg
     2641 ccgagggcag gcacagcacc ggcggcatgg acgagctgta caagctcaga tctcgagctg
     2701 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga
     2761 agagaaaggt aggatccacc ggatctagcc atggcttccc gccggaggtg gaggagcagg
     2821 atgatggcac gctgcccatg tcttgtgccc aggagagcgg gatggaccgt caccctgcag
     2881 cctgtgcttc tgctaggatc aatgtgtaga cccagctttc ttgtacaaag tggtgatacc
     2941 cagctttctt gtacaaagtg gtgatcctca ggtgcaggct gcctatcaga aggtggtggc
     3001 tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa
     3061 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     3121 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     3181 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     3241 tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc
     3301 ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat
     3361 attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact
     3421 agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg
     3481 gagatccctc gacctgcagc ccaagcttcg cgttgacatt gattattgac tagttattaa
     3541 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     3601 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     3661 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     3721 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     3781 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     3841 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     3901 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     3961 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     4021 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     4081 gtctatataa gcagagctct ctggctaact agagaaccca ctgcgccacc atgattgaac
     4141 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4201 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4261 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     4321 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4381 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4441 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4501 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4561 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4621 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     4681 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4741 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4801 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4861 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4921 tctgactcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4981 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     5041 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     5101 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     5161 gcaggcatgc tggggatgcg gtgggctcta tggctcgagt taattaacga gagcataata
     5221 ttgatatgtg ccaaagttgt ttctgactga ctaataagta taatttgttt ctattatgta
     5281 taggttaagc taattactta ttttataata caacatgact gtttttaaag tacaaaataa
     5341 gtttattttt gtaaaagaga gaatgtttaa aagttttgtt actttataga agaaattttg
     5401 agtttttgtt tttttttaat aaataaataa acataaataa attgtttgtt gaatttatta
     5461 ttagtatgta agtgtaaata taataaaact taatatctat tcaaattaat aaataaacct
     5521 cgatatacag accgataaaa cacatgcgtc aattttacgc atgattatct ttaacgtacg
     5581 tcacaatatg attatctttc tagggttaaa taatagtttc taattttttt attattcagc
     5641 ctgctgtcgt gaataccgag ctccaattcg ccctatagtg agtcgtatta caattcactg
     5701 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5761 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5821 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     5881 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5941 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6001 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6061 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6121 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6181 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6241 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6301 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6361 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6421 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6481 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6541 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6601 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6661 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6721 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6781 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6841 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6901 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6961 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7021 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7081 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7141 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7201 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7261 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7321 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7381 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7441 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7501 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7561 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7621 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7681 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7741 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7801 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7861 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7921 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7981 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8041 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8101 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8161 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8221 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8281 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8341 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     8401 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     8461 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggtacctc
     8521 gcgcgacttg gtttgccatt ctttagcgcg cgtcgcgtca cacagcttgg ccacaatgtg
     8581 gtttttgtca aacgaagatt ctatgacgtg tttaaagttt aggtcgagta aagcgcaaat
     8641 ctttt
//
