LOCUS       sequence-401022-        9808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9808)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-PTPRF-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             5384..5428
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5429..5446
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5586..5603)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5592..5703
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5821..5841)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5826..6155
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6006..6141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6068..6087
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6203..6250
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6269..6868
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6269..6288)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6765..6785
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6998..7119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7035..7054)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7089..7108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(7172..7963,7964..8032))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7795..7814
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8033..8124)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8166..8185
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8191..8210
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8296..8884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8785..8804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9038..9055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     9153..9170
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9154..9170
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9626..9642
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg ccgatagcaa gcctgtgttt atcaaggtgc ccgaggacca
     1021 gactggactg tctggcggag ttgccagctt tgtgtgtcag gccacaggcg agcctaagcc
     1081 tagaatcacc tggatgaaga aaggcaagaa ggtgtccagc cagcgcttcg aagtgatcga
     1141 gtttgatgat ggcgccggaa gcgtgctgag aatccagcct ctgagagtgc agagagatga
     1201 ggccatctac gagtgcaccg ccacaaatag cctgggcgag atcaatacca gcgccaagct
     1261 gagcgtgctg gaagaggaac agctgcctcc tggcttcccc tctatcgata tgggacctca
     1321 gctgaaggtg gtggaaaagg ccagaaccgc cactatgctt tgtgccgctg gcggcaatcc
     1381 tgatcctgag atcagctggt tcaaggactt cctgcctgtg gaccctgcca ccagcaatgg
     1441 cagaatcaag cagctgagat ctggcgccct gcagatcgag agcagcgagg aaagcgatca
     1501 gggcaaatac gagtgcgtgg ccaccaatag cgccggcaca agatattctg cccctgccaa
     1561 cctgtacgtc agagtgcgaa gagtggcccc tagattcagc atccctccta gcagccaaga
     1621 agtgatgcct ggcggctccg tgaatctgac atgtgtggct gttggcgccc ctatgcctta
     1681 cgtgaaatgg atgatgggag ccgaggaact gaccaaagag gacgagatgc ccgtgggcag
     1741 aaatgtgctg gaactgagca atgtcgtgcg gagcgccaat tacacctgtg tggccatcag
     1801 cagcctgggc atgattgagg ccacagctca agtgacagtg aaggccctgc ctaagccacc
     1861 tatcgacctg gtggttaccg agacaaccgc cacctctgtg accctgacat gggatagcgg
     1921 caatagcgag ccagtgacct actacggcat ccagtataga gccgccggaa cagagggccc
     1981 ttttcaagaa gttgacggcg tcgccaccac cagatatagc attggcggac tgagcccctt
     2041 cagcgagtac gcctttagag tgctggccgt gaactctatc ggcagaggac ctccatctga
     2101 ggccgttaga gctagaacag gcgaacaggc cccttctagc cctcctagaa gagtgcaggc
     2161 tagaatgctg agcgccagca caatgctggt gcagtgggaa cctcctgagg aacccaatgg
     2221 cctcgtgcgg ggctacagag tgtactacac acccgatagc agacggcctc ctaacgcctg
     2281 gcacaagcac aatactgatg ccggcctgct gacaaccgtg ggatctctgc tgcctggcat
     2341 cacctatagc ctgcgcgtgc tggcttttac cgccgttgga gatggaccac cttctccaac
     2401 catccaagtg aaaacccagc agggcgttcc agctcagcct gccgattttc aggccgaggt
     2461 ggaaagcgac acccgcattc aacttagctg gctgctgcct ccacaagagc ggatcattat
     2521 gtacgagctg gtgtactggg ccgctgagga tgaggatcag cagcacaaag tgaccttcga
     2581 tcccaccagc agctacaccc tggaagatct gaagcccgac acactgtaca gattccagct
     2641 ggccgctaga agcgatatgg gcgtgggagt gtttacccct accatcgaag ccagaacagc
     2701 ccagagcaca cctagcgctc ctccacagaa agtgatgtgc gtgtccatgg gcagcaccac
     2761 agtcagagtg tcttgggtgc cacctcctgc cgacagcaga aatggcgtga tcacacagta
     2821 cagcgtggcc tatgaagccg tggatggcga ggatagaggc agacacgtgg tggatggaat
     2881 cagcagagag cacagctctt gggatctcgt gggcctcgag aagtggaccg agtatagagt
     2941 gtgggtccga gcacacacag atgtcggacc tggacctgaa agcagccctg tgctcgtgcg
     3001 gacagatgag gatgttccta gcggacctcc tcggaaggtg gaagtggaac ctctgaatag
     3061 caccgccgtg cacgtgtact ggaagctgcc tgtgccttct aagcagcacg gccagatcag
     3121 aggctaccaa gtgacatacg tgcggctgga aaatggcgag ccaagaggcc tgcctatcat
     3181 ccaggatgtg atgctggctg aagcccagtg gcggcctgag gaatctgagg actacgaaac
     3241 caccatcagc gggctgaccc ctgagacaac atactctgtg acagtggccg cctacaccac
     3301 aaaaggcgac ggcgctagat ccaagcctaa gatcgtgacc acaacaggcg ctgtgcctgg
     3361 cagacctacc atgatgatca gcaccaccgc catgaacaca gccctgctgc agtggcaccc
     3421 tcctaaagaa ctgcctggcg agctgctggg ctatagactg cagtactgta gagccgacga
     3481 ggccagacct aacaccatcg acttcggcaa ggacgaccag cactttacag tgaccggcct
     3541 gcacaagggc accacctaca tttttagact ggccgccaag aacagagccg gcctgggaga
     3601 agagttcgag aaagagatca gaaccccaga ggatctgccc agcggcttcc ctcagaatct
     3661 gcacgtgaca ggcctgacca cctctaccac agaacttgct tgggaccctc cagtgctggc
     3721 agagagaaat ggccggatca tctcctacac cgtggtgttc agagacatca acagccagca
     3781 agagctgcag aacatcacca ccgacaccag attcaccctg accggactga agcctgatac
     3841 cacctacgac atcaaagtcc gcgcctggac cagcaaaggc tctggacctc tgagccctag
     3901 catccagagc agaaccatgc cagtggaaca ggtgttcgcc aagaatttca gagtggccgc
     3961 tgccatgaag accagcgtgc tgttgtcttg ggaagtgccc gacagctaca agagcgccgt
     4021 gccattcaag atcctgtaca acggccagag cgttgaggtg gacggccaca gcatgagaaa
     4081 gctgatcgcc gacctgcagc ctaacaccga gtacagcttc gtgctgatga acagaggaag
     4141 ctctgccggc ggactgcagc acctggtgtc tatcagaaca gctcccgacc tgctgccaca
     4201 caaacctctg cctgccagcg cctatatcga ggacggcaga ttcgatctga gcatgccaca
     4261 cgtgcaggac ccttctctcg tgcggtggtt ttacatcgtg gtggtgccta tcgacagagt
     4321 cggcggatct atgctgaccc caagatggtc cacaccagag gaactggaac tggacgaact
     4381 gctggaagcc atcgagcaag gcggcgagga acaacgcaga agaagaaggc aggccgagcg
     4441 cctgaaacct tatgtggctg ctcaactgga cgtgctgccc gagacattca cactgggcga
     4501 caagaagaac taccggggct tctacaaccg gccactgagc cctgatctga gctaccagtg
     4561 tttcgtgctg gcctctctga aagaacccat ggaccagaag agatacgcca gctctcccta
     4621 cagcgacgag atcgtggtgc aagtgactcc cgctcagcag caagaggaac ccgaaatgct
     4681 ttggggcgcg ccgggcggcg acaagaccca cacctgcccc ccctgcccag ctccagaact
     4741 gctgggaggg ccttccgtgt tcctgttccc ccccaagccc aaggacaccc tgatgatcag
     4801 caggaccccc gaggtgacct gcgtggtggt ggccgtgtcc cacgaggacc cagaggtgaa
     4861 gttcaactgg tacgtggacg gcgtggaggt gcacaacgcc aagaccaagc ccagagagga
     4921 gcagtacaac agcacctaca gggtggtgtc cgtgctgacc gtgctgcacc aggactggct
     4981 gaacggcaaa gaatacaagt gcaaagtctc caacaaggcc ctggctgccc caatcgaaaa
     5041 gacaatcagc aaggccaagg gccagccacg ggagccccag gtgtacaccc tgccccccag
     5101 ccgggaggag atgaccaaga accaggtgtc cctgacctgt ctggtgaagg gcttctaccc
     5161 cagcgatatc gccgtggagt gggagagcaa cggccagccc gagaacaact acaagaccac
     5221 ccccccagtg ctggacagcg acggcagctt cttcctgtac agcaagctga ccgtggacaa
     5281 gtccaggtgg cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa
     5341 ccactacacc cagaagtccc tgagcctgag ccccggcggc agcggtctta atgatatttt
     5401 cgaggctcag aagattgagt ggcatgaaca ccaccaccat caccactaag gccggcctgt
     5461 agtcactggc acaacgcgtt acccggtaag ccaatcgggt atacacggtc gtcatactgc
     5521 agacagggtt cttctacttt gcaagatagt cttgagtagt aaaataaata gatagagaaa
     5581 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5641 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5701 atcacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5761 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt
     5821 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5881 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     5941 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6001 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6061 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt
     6121 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca
     6181 ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt
     6241 ataatacgac aaggtgagga actaaaccat gaccgagtac aagcccacgg tgcgcctcgc
     6301 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     6361 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     6421 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     6481 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     6541 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     6601 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     6661 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     6721 cgagcgcgcc ggggtgcccg ccttcctgga gacatccgcg ccccgcaacc tccccttcta
     6781 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     6841 gtgcatgacc cgcaagcccg gtgcctgaca cgtgctacga gatttcgatt ccaccgccgc
     6901 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6961 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     7021 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7081 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     7141 ctctagctag agcttggcgt aatcatggtc attaccaatg cttaatcagt gaggcaccta
     7201 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7261 ctacgatacg ggagggctta ccatctggcc ccagcgctgc gatgataccg cgagaaccac
     7321 gctcaccggc tccggattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7381 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     7441 taagtagttc gccagttaat agtttgcgca acgttgttgc catcgctaca ggcatcgtgg
     7501 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     7561 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     7621 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     7681 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     7741 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     7801 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     7861 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     7921 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     7981 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atattcttcc
     8041 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8101 aatgtattta gaaaaataaa caaatagggg tcagtgttac aaccaattaa ccaattctga
     8161 acattatcgc gagcccattt atacctgaat atggctcata acaccccttg ctcatgacca
     8221 aaatccctta acgtgagtta cgcgcgcgtc gttccactga gcgtcagacc ccgtagaaaa
     8281 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8341 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8401 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8461 gttagcccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8521 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     8581 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     8641 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     8701 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8761 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8821 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8881 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8941 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9001 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9061 ggaaggcgag agtagggaac tgccaggcat caaactaagc agaaggcccc tgacggatgg
     9121 cctttttgcg tttctacaaa ctctttctgt gttgtaaaac gacggccagt cttaagctcg
     9181 ggccccctgg gcggttctga taacgagtaa tcgttaatcc gcaaataacg taaaaacccg
     9241 cttcggcggg tttttttatg gggggagttt agggaaagag catttgtcag aatatttaag
     9301 ggcgcctgtc actttgcttg atatatgaga attatttaac cttataaatg agaaaaaagc
     9361 aacgcacttt aaataagata cgttgctttt tcgattgatg aacacctata attaaactat
     9421 tcatctatta tttatgattt tttgtatata caatatttct agtttgttaa agagaattaa
     9481 gaaaataaat ctcgaaaata ataaagggaa aatcagtttt tgatatcaaa attatacatg
     9541 tcaacgataa tacaaaatat aatacaaact ataagatgtt atcagtattt attatcattt
     9601 agaataaatt ttgtgtcgcc cttaattgtg agcggataac aattacgagc ttcatgcaca
     9661 gtggcgttga ctcaatattg gccattagcc atattattca ttggttatat agcataaatc
     9721 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
     9781 ggctcatgtc caatatgacc gccatgtt
//
