LOCUS       sequence-401028-        9272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsRoquin1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Sgromo A, Raisch T, Bawankar P, Bhandari D, Chen Y, Kuzuoglu-Ozturk 
            D, Weichenrieder O, Izaurralde E
  TITLE     A CAF40-binding motif facilitates recruitment of the CCR4-NOT 
            complex to mRNAs targeted by Drosophila Roquin.
  JOURNAL   Nat Commun. 2017 Feb 6;8:14307. doi: 10.1038/ncomms14307.
  PUBMED    28165457
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-02-6"; volume: "8:14307. doi"; pages: " 
            10.1038/ncomms14307"
COMMENT     Sequence Label: pcDNA3.1-MS2-HA-HsRoquin1_AE
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      895..904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1333..1359
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             3618..3626
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(4825..4843)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4826..4843)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4863..4880)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4869..5093
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5139..5567
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5226..5245)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5436..5457
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5576..5596)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5581..5910
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5761..5896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5823..5842
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5977..6771
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6031..6050)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6641..6660
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6945..7066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6982..7001)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7036..7055
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7115..7131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7115..7131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7128..7150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7139..7155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7163..7169,7170..7187,7188..7193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7208..7229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7346..7363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7517..8105)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7597..7616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8276..9067,9068..9136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8899..8918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9137..9241)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgccacc
      901 atggcttcta actttactca gttcgttctc gtcgacaatg gcggaactgg cgacgtgact
      961 gtcgccccaa gcaacttcgc taacggggtc gctgaatgga tcagctctaa ctcgcgatca
     1021 caggcttaca aagtaacctg tagcgttcgt cagagctctg cgcagaatcg caaatacacc
     1081 atcaaagtcg aggtgcctaa agtggcaacc cagactgttg gtggtgaaga gcttcctgta
     1141 gccggatgga gatcttactt aaatatggaa ctaaccattc caattttcgc cacgaattcc
     1201 gactgcgagc ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc
     1261 tcggccatcg cggccaactc cggcatctac ggaggtggag gtggatctgg tggatctcct
     1321 ccacatatgg cttacccata cgatgttcca gattacgctc ctccaaagct tggatccgaa
     1381 ttcccgggtc gactcgagtc tagagcggcc gccatgcctg tacaagctcc acaatggacg
     1441 gatttcctct cctgcccaat ttgcactcag actttcgacg aaacaattcg aaagcccatc
     1501 agtttgggtt gtggccatac tgtctgcaag atgtgcctga ataaactcca ccgcaaggct
     1561 tgcccatttg accagaccac tatcaataca gacattgagc tcctccctgt gaactcagca
     1621 ttgctgcagc tcgtgggtgc tcaggtccct gagcagcagc ctattacttt gtgtagtggg
     1681 gttgaagaca caaagcatta tgaggaagcc aagaaatgtg tagaagaatt agcattgtac
     1741 ttaaaaccgc tcagcagtgc tagaggagtg ggtctgaaca gcactactca gagtgttctg
     1801 agtcgcccaa tgcagaggaa gcttgtgact ctagtccact gtcaactagt agaagaagaa
     1861 ggcaggattc gtgccatgag ggcagctcga tctttaggtg aacgaacagt tacagagctc
     1921 attctccagc accagaatcc tcagcaactc tcttccaatc tttgggcagc agtaagggct
     1981 aggggatgcc agttccttgg accagcaatg caggaggaag ctttaaagtt ggttctgctg
     2041 gccttagaag atggttctgc tttgtcaaga aaagtattgg ttctgtttgt ggtgcaaaga
     2101 ttggagccac ggtttcctca agcctctaaa actagcattg ggcatgttgt tcagctcctt
     2161 tatagagcct cctgtttcaa ggtcaccaaa cgtgatgaag actcttcttt gatgcagctg
     2221 aaagaagaat ttagaaccta tgaagctctg cggcgagaac atgactccca gatagtgcag
     2281 attgctatgg aagcaggctt acgaattgca ccggaccagt ggtcctcttt gctttatgga
     2341 gaccagtctc acaaatctca tatgcagtcc attattgaca agttgcagac tccagcctct
     2401 tttgcacaga gtgttcagga actaacaatt gctctccagc gaactggaga cccagcaaac
     2461 ttgaaccgac taagacccca tttggagctg ttagcaaaca ttgaccctag tccagatgct
     2521 cctcctccta cttgggaaca gctggaaaat gggctggttg ctgtgcgtac agtggtacat
     2581 gggctggttg attatatcca gaaccacagc aaaaaaggag cagatcagca gcagcctcca
     2641 cagcatagca aatacaaaac atacatgtgt cgagatatga agcagagagg aggatgccct
     2701 cgtggggcca gctgtacatt tgcacactca caggaggaac tggaaaaatt tcgtaaaatg
     2761 aataagcgcc tggttccgag aagacccttg agtgcctctt tgggtcaact taatgaggtg
     2821 ggcctgcctt cagcagctat ccttccagat gaaggtgcag tggatctccc tagcagaaaa
     2881 cctcctgctc tgccaaatgg aattgtatca acagggaata cagtaacaca gcttattccg
     2941 cgagggacag accccagcta tgattctagt ctgaaaccag gaaaaataga tcatctgagc
     3001 agtagtgctc ctggatcccc tcctgacctg ctagaatctg ttcctaagag tatttctgcc
     3061 ttacctgtga atccacattc tatacctcca agggggccag cagatctgcc tccaatgcct
     3121 gttaccaaac cacttcagat ggtacctcga ggttctcagt tatatccagc acaacagacg
     3181 gatgtttatt atcaggatcc tcgaggagca gctccgccat ttgaaccagc accttatcag
     3241 cagggtatgt attatactcc accaccacaa tgtgtgtccc gctttgtccg acctccacca
     3301 tctgctcctg aacctgctcc tccctacttg gatcattatc caccctacct ccaagaacgt
     3361 gttgtaaact ctcagtatgg cacacagcca cagcagtacc cacctatata cccatctcac
     3421 tatgatggcc gtcgagtgta ccctgctccg tcttacacaa gagaagagat attccgagaa
     3481 agccctatac ccattgagat tccacctgca gcagtaccat cgtatgtacc agaatccaga
     3541 gaaagatacc aacagatcga gagttactat ccagtggctc ctcatccaac tcagatcaga
     3601 ccttcgtacc tcagagaacc tccttatagc cggcttcctc ctcctcccca gcctcatcct
     3661 agtctagatg aactacatcg ccgacgaaag gaaataatgg cccagctaga ggaaagaaag
     3721 gttatctctc cacctccttt tgcaccttca ccaaccttgc ctcctacctt tcatccggaa
     3781 gaatttttgg atgaagactt gaaggtagct gggaaataca aaggaaatga ttatagccaa
     3841 tactctccct ggtcatgtga caccatcggc tcctacattg gaaccaaaga tgcaaaaccc
     3901 aaagatgttg tggcagcagg gagtgtggaa atgatgaatg tggagagtaa aggaatgagg
     3961 gaccagcgat tagatcttca gagaagagca gcagaaacca gtgatgatga cctcatccca
     4021 tttggagacc gaccaacagt gtctcggttt ggtgccatct cacgaacttc caaaactata
     4081 tatcagggtg ctggtccaat gcaggctatg gcacctcagg gagctcctac aaaatctatt
     4141 aacatttcag attatagtcc atatggaacc cacggtggct ggggagcttc tccatattca
     4201 cctcatcaaa acataccttc tcagggacac ttcagtgaga gggagagaat atctatgtca
     4261 gaagtggcca gtcatggaaa accccttcca tctgctgaga gagaacagct acgactagaa
     4321 ttgcagcaat tgaaccatca gattagccag cagacccagc tacgtggact agaggctgtt
     4381 agtaacaggc tggtgttgca gagggaggca aacaccctgg caggccagtc acagccccca
     4441 ccaccgccac ctccaaaatg gcctgggatg atctcaagtg agcagttgag cttggaactg
     4501 caccaggtgg aaagggaaat cgggaagaga acacgggaac tgagtatgga aaaccagtgt
     4561 tctctggaca tgaaaagcaa actgaataca agtaaacaag cagaaaatgg acaaccagaa
     4621 ccacaaaaca aggttccggc tgaggacctt acattgacat tcagtgatgt accaaatgga
     4681 tcagccttga cacaagagaa tatcagcctc ctatcaaaca agaccagctc tctgaacctg
     4741 tcagaggacc ctgagggagg aggggataat aatgactccc agagatcagg agttactccc
     4801 agttctgctc cttaagggcc ctattctata gtgtcaccta aatgctagag ctcgctgatc
     4861 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     4921 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     4981 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5041 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5101 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5161 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5221 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5281 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5341 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5401 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5461 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     5521 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5581 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5641 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     5701 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     5761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     5821 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     5881 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     5941 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     6001 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     6061 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     6121 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     6181 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     6241 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     6301 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     6361 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     6421 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     6481 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     6541 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     6601 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     6661 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     6721 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     6781 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     6841 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     6901 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     6961 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7021 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7081 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7141 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7201 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7261 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7321 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7381 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7441 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7501 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7561 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7621 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7681 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7741 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7801 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7861 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7921 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7981 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8041 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8101 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8161 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8221 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8281 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8341 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     8401 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     8461 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     8521 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8581 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8641 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8701 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8761 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8821 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8881 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8941 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9001 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9061 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9121 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9181 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9241 gcacatttcc ccgaaaagtg ccacctgacg tc
//
