LOCUS       sequence-401034-        9322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cell-surface protein extracellular domain 
            fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9322)
  AUTHORS   Wojtowicz WM, Vielmetter J, Fernandes RA, Siepe DH, Eastman CL, 
            Chisholm GB, Cox S, Klock H, Anderson PW, Rue SM, Miller JJ, Glaser 
            SM, Bragstad ML, Vance J, Lam AW, Lesley SA, Zinn K, Garcia KC
  TITLE     A Human IgSF Cell-Surface Interactome Reveals a Complex Network of 
            Protein-Protein Interactions.
  JOURNAL   Cell. 2020 Aug 20;182(4):1027-1043.e17. doi: 
            10.1016/j.cell.2020.07.025.
  PUBMED    32822567
REFERENCE   2  (bases 1 to 9322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.07"; journalName: "Cell"; date: "2020-08-20- 
            20"; volume: "182"; issue: "4"; pages: "1027-1043.e17"
COMMENT     Sequence Label: pD649-HAsp-DCC-Fc(DAPA)-AviTag-6xHis
FEATURES             Location/Qualifiers
     source          1..9322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        77..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     815..832
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     846..865
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             4898..4942
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             4943..4960
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5100..5117)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5106..5217
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5335..5355)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5340..5669
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5520..5655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5582..5601
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5717..5764
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5783..6382
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5783..5802)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6279..6299
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6512..6633
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6549..6568)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6603..6622
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6686..7477,7478..7546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7309..7328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7547..7638)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7680..7699
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7705..7724
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7810..8398
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8299..8318
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8552..8569
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8667..8684
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8668..8684
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9140..9156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggcattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat
      901 cgaaaacgcg cctgctagcg ccaccatgaa gaccatcatt gccctgagct acatcttctg
      961 cctggtgttc gctgcggccg cccatctgca agtgacagga ttccagatca aggccttcac
     1021 agccctgaga tttctgagcg agcctagcga cgctgtgacc atgagaggtg gcaatgtgct
     1081 gctggattgc agcgccgaat ctgatagagg cgtgcccgtg atcaagtgga agaaggatgg
     1141 aatccatctg gccctcggca tggacgagag aaagcagcag ctgagcaatg gcagcctgct
     1201 gatccagaac atcctgcaca gcagacacca caagcctgac gagggcctgt accagtgtga
     1261 agcctctctg ggcgatagcg gcagcatcat cagcagaaca gccaaagtgg ctgtggccgg
     1321 acctctgaga ttcctgtctc agacagagtc cgtgaccgcc ttcatgggcg atacagtgct
     1381 gctgaagtgc gaagtgatcg gcgagcctat gcctaccatc cactggcaga agaaccagca
     1441 ggatctgacc cctattcctg gcgacagtag agtggtggtt ctgccttctg gcgccctgca
     1501 gattagtaga ctgcagcctg gcgatatcgg catctaccgg tgttctgcca gaaatcccgc
     1561 cagcagcaga accggaaatg aggccgaagt gcgcatcctg tctgatcctg gactgcaccg
     1621 gcagctgtac tttctgcaga ggcctagcaa cgtggtggcc atcgagggaa aagatgccgt
     1681 gctggaatgc tgcgtgtccg gctatcctcc acctagcttt acctggctga gaggcgagga
     1741 agtgatccag ctgcggagca agaagtatag cctgctcggc ggcagcaatc tgctgatctc
     1801 caacgtgacc gacgacgaca gcggcatgta cacctgtgtg gtcacctaca agaacgagaa
     1861 catcagcgcc agcgccgagc tgacagttct ggttcctcca tggttcctga atcaccccag
     1921 caacctgtac gcctacgaga gcatggacat cgagttcgag tgcaccgtgt ctggcaagcc
     1981 cgtgcctacc gtgaactgga tgaagaacgg cgacgtggtc atccccagcg actactttca
     2041 gatcgtcggc ggctccaacc tgagaatcct gggcgttgtg aagtccgatg agggctttta
     2101 tcagtgcgtg gccgagaacg aagccggcaa tgctcagaca tctgcccagc tgattgtgcc
     2161 caagcctgcc attcctagca gctctgtgct gccctctgct cccagagatg tggtgcctgt
     2221 gctggtgtct agcagatttg tgcggctgtc ttggaggcca ccagccgagg ctaagggcaa
     2281 tatccagacc ttcaccgtgt tcttcagcag agagggcgac aacagagagc gggccctgaa
     2341 cacaacacag cctggatctc tgcagctgac cgtgggcaat ctgaagcccg aggccatgta
     2401 cacattccgc gtggtggctt acaatgagtg gggccctggc gagtctagcc agcctatcaa
     2461 agtggccaca cagcccgaac tgcaggtccc cggacctgtt gaaaatctcc aggccgtgtc
     2521 tacaagcccc accagcatcc tgattacatg ggagcctcca gcctacgcca atggacctgt
     2581 gcagggctac agactgttct gcaccgaggt gtccaccggc aaagaacaga atatcgaggt
     2641 ggacggcctg agctacaagc tggaaggcct gaagaagttc accgagtaca gcctgcggtt
     2701 cctggcctac aatagatacg gacccggcgt gtccacagac gacatcacag tggtcacact
     2761 gagcgacgtg ccaagcgctc ctcctcagaa tgtgtccctg gaagtggtca acagccggtc
     2821 cattaaggtg tcctggctgc ctcctccaag cggcacccag aatggcttta tcaccggcta
     2881 caagatccgg cacagaaaga ccacaagacg gggcgagatg gaaaccctgg aacctaacaa
     2941 cctgtggtat ctgttcaccg gcctggaaaa gggcagccag tacagctttc aggtgtccgc
     3001 catgaccgtg aatggcacag gcccacctag caattggtac accgccgaga cacccgagaa
     3061 tgacctggat gagtctcagg tgccagacca gccttcttct ctgcatgtgc ggccccagac
     3121 caactgcatc atcatgtctt ggacccctcc actgaacccc aatatcgtcg tgcggggcta
     3181 catcatcggc tatggcgtgg gatctcctta cgccgaaacc gtcagagtgg acagcaagca
     3241 gcggtactac agcatcgaga gactggaaag cagctcccac tacgtgatca gcctgaaagc
     3301 cttcaacaat gccggcgagg gcgtgccact gtacgaaagc gccaccacca gatccatcac
     3361 cgatcctacc gatccagtgg actactaccc tctgctggac gactttccta ccagcgtgcc
     3421 agatctgtct acccctatgc tgcctccagt gggagttcag gctgtggctc tgacacacga
     3481 tgccgttaga gtgtcctggg ccgacaacag cgtgcccaag aaccagaaaa caagcgaagt
     3541 ccggctgtac accgtgcggt ggcgaacaag ctttagcgcc tctgccaagt acaagagcga
     3601 ggataccacc agcctgtcct acaccgccac cggactgaag cccaacacta tgtacgagtt
     3661 cagcgtgatg gtcaccaaga accggcggag cagcacttgg tctatgacag cccacgccac
     3721 cacatacgag gccgctccta catctgctcc taaggacctg accgtgatca caagagaggg
     3781 caagcctaga gccgtgatcg tgtcttggca acctcctctg gaagccaacg gcaagatcac
     3841 cgcctacatc ctgttctaca ccctggacaa gaacatcccc atcgacgact ggatcatgga
     3901 aaccatcagc ggcgaccggc tgacacacca gatcatggac ctgaacctgg acaccatgta
     3961 ctacttcaga attcaggccc ggaacagcaa aggcgtggga cctctcagcg accctatcct
     4021 gtttcggacc ctgaaggtgg aacaccccga caagatggcc aacgatcaag gcagacatgg
     4081 cgacggcgga tactggcctg tggataccaa cctgatcgac agaagcaccc tgaacgagcc
     4141 tccaatcgga cagatgcacc cacctcacgg aagcgtgacc cctcagaaaa acagcaatgg
     4201 cgcgccgggc ggcgacaaga cccacacctg ccccccctgc ccagctccag aactgctggg
     4261 agggccttcc gtgttcctgt tcccccccaa gcccaaggac accctgatga tcagcaggac
     4321 ccccgaggtg acctgcgtgg tggtggccgt gtcccacgag gacccagagg tgaagttcaa
     4381 ctggtacgtg gacggcgtgg aggtgcacaa cgccaagacc aagcccagag aggagcagta
     4441 caacagcacc tacagggtgg tgtccgtgct gaccgtgctg caccaggact ggctgaacgg
     4501 caaagaatac aagtgcaaag tctccaacaa ggccctggct gccccaatcg aaaagacaat
     4561 cagcaaggcc aagggccagc cacgggagcc ccaggtgtac accctgcccc ccagccggga
     4621 ggagatgacc aagaaccagg tgtccctgac ctgtctggtg aagggcttct accccagcga
     4681 tatcgccgtg gagtgggaga gcaacggcca gcccgagaac aactacaaga ccaccccccc
     4741 agtgctggac agcgacggca gcttcttcct gtacagcaag ctgaccgtgg acaagtccag
     4801 gtggcagcag ggcaacgtgt tcagctgcag cgtgatgcac gaggccctgc acaaccacta
     4861 cacccagaag tccctgagcc tgagccccgg cggcagcggt cttaatgata ttttcgaggc
     4921 tcagaagatt gagtggcatg aacaccacca ccatcaccac taaggccggc ctgtagtcac
     4981 tggcacaacg cgttacccgg taagccaatc gggtatacac ggtcgtcata ctgcagacag
     5041 ggttcttcta ctttgcaaga tagtcttgag tagtaaaata aatagataga gaaaatcagc
     5101 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     5161 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcaca
     5221 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5281 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     5341 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5401 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5461 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5521 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5581 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5641 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5701 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     5761 cgacaaggtg aggaactaaa ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5821 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5881 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5941 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     6001 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     6061 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     6121 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     6181 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     6241 cgccggggtg cccgccttcc tggagacatc cgcgccccgc aacctcccct tctacgagcg
     6301 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     6361 gacccgcaag cccggtgcct gacacgtgct acgagatttc gattccaccg ccgccttcta
     6421 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6481 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6541 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6601 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6661 ctagagcttg gcgtaatcat ggtcattacc aatgcttaat cagtgaggca cctatctcag
     6721 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6781 tacgggaggg cttaccatct ggccccagcg ctgcgatgat accgcgagaa ccacgctcac
     6841 cggctccgga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6901 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6961 gttcgccagt taatagtttg cgcaacgttg ttgccatcgc tacaggcatc gtggtgtcac
     7021 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7081 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7141 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7201 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7261 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7321 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7381 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7441 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7501 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatattc ttcctttttc
     7561 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7621 tttagaaaaa taaacaaata ggggtcagtg ttacaaccaa ttaaccaatt ctgaacatta
     7681 tcgcgagccc atttatacct gaatatggct cataacaccc cttgctcatg accaaaatcc
     7741 cttaacgtga gttacgcgcg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7801 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7861 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7921 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagc
     7981 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8041 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8101 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8161 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8221 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8281 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8341 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8401 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8461 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8521 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaagg
     8581 cgagagtagg gaactgccag gcatcaaact aagcagaagg cccctgacgg atggcctttt
     8641 tgcgtttcta caaactcttt ctgtgttgta aaacgacggc cagtcttaag ctcgggcccc
     8701 ctgggcggtt ctgataacga gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg
     8761 cgggtttttt tatgggggga gtttagggaa agagcatttg tcagaatatt taagggcgcc
     8821 tgtcactttg cttgatatat gagaattatt taaccttata aatgagaaaa aagcaacgca
     8881 ctttaaataa gatacgttgc tttttcgatt gatgaacacc tataattaaa ctattcatct
     8941 attatttatg attttttgta tatacaatat ttctagtttg ttaaagagaa ttaagaaaat
     9001 aaatctcgaa aataataaag ggaaaatcag tttttgatat caaaattata catgtcaacg
     9061 ataatacaaa atataataca aactataaga tgttatcagt atttattatc atttagaata
     9121 aattttgtgt cgcccttaat tgtgagcgga taacaattac gagcttcatg cacagtggcg
     9181 ttgactcaat attggccatt agccatatta ttcattggtt atatagcata aatcaatatt
     9241 ggctattggc cattgcatac gttgtatcta tatcataata tgtacattta tattggctca
     9301 tgtccaatat gaccgccatg tt
//
