LOCUS       sequence-401038-        9301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsEDC4. Please note that this plasmid does 
            not contain the T7 promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Braun JE, Tritschler F, Haas G, Igreja C, Truffault V, Weichenrieder
            O, Izaurralde E
  TITLE     The C-terminal alpha-alpha superhelix of Pat is required for mRNA 
            decapping in metazoa.
  JOURNAL   EMBO J. 2010 Jul 21. 29(14):2368-80.
  PUBMED    20543818
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2010 Jul 21. 29(14):2368-80."
COMMENT     Sequence Label: pT7-EGFP-C1-HsEDC4_S
FEATURES             Location/Qualifiers
     source          1..9301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6089..6210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6126..6145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6180..6199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6217..6672)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6354..6375)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6566..6585
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6699..6803
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6805..7162
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6805..6996
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6828..6848)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7013..7148
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7197..7991
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7197..7991
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7251..7270)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7861..7880
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8179..8198)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8223..8270
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8599..9187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9088..9107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggccggac tcagatctcg agctcaagct tcgaattcat ggcctcctgc
     1381 gcgagcatcg acatcgagga cgccacgcag cacctgcggg acatcctcaa gctggaccgg
     1441 cccgcgggcg gccccagtgc agagagccca cggccatcca gtgcctacaa tggggacctc
     1501 aatggacttc tggtcccaga cccgctctgc tcaggtgata gtacctcagc aaacaagact
     1561 ggtcttcgga ccatgccacc cattaacctg caagagaagc aggtcatctg tctctcagga
     1621 gatgatagct ccacctgcat tgggattttg gccaaggagg tggagattgt ggctagcagt
     1681 gactctagca tttcaagcaa ggcccgggga agcaacaagg tgaaaattca gcctgtcgcc
     1741 aagtatgact gggaacagaa gtactactat ggcaacctga ttgctgtgtc taactccttc
     1801 ttggcctatg ccattcgggc tgccaacaat ggctctgcca tggtgcgggt gatcagcgtc
     1861 agcacttcgg agcggacctt gctcaagggc ttcacaggca gtgtggctga tctggctttc
     1921 gcgcacctca actctccaca gctggcctgc ctggatgagg caggcaacct gttcgtgtgg
     1981 cgcttggctc tggttaatgg caaaattcaa gaagagatct tggtccatat tcggcagcca
     2041 gagggcacgc cactgaacca ctttcgcagg atcatctggt gccccttcat ccctgaggag
     2101 agcgaagact gctgtgagga gagcagccca acagtggccc tgctgcatga agaccgggct
     2161 gaggtgtggg acctggacat gctccgctcc agccacagta cctggcctgt ggatgttagc
     2221 cagatcaagc agggcttcat tgtggtaaaa ggtcatagca cgtgcctcag tgaaggagcc
     2281 ctctctcctg atgggactgt gctggctact gcgagccacg atggctatgt caagttctgg
     2341 cagatctaca ttgaggggca agatgagcca aggtgtctgc acgagtggaa acctcatgat
     2401 gggcggcccc tctcctgcct cctgttctgt gacaaccata agaaacaaga ccctgatgtc
     2461 cctttctgga ggttccttat tactggtgct gaccagaacc gagagttaaa gatgtggtgt
     2521 acagtatcct ggacctgcct gcagactatt cgcttctccc cagatatctt cagctcagtg
     2581 agtgtgcccc ctagcctcaa ggtttgcttg gacctctcag cagaatacct gattctcagc
     2641 gatgtgcaac ggaaggtcct ctatgtgatg gagctgctgc aaaaccagga ggagggccac
     2701 gcctgcttca gctccatctc ggagttcctg ctcacccacc ctgtgctgag ctttggtatc
     2761 caggttgtga gtcgctgccg gctacggcac actgaggtgc tgcctgccga agaggaaaat
     2821 gacagcctgg gtgctgatgg tacccatgga gccggtgcca tggagtctgc ggccggtgtg
     2881 ctcatcaagc tcttttgtgt gcatactaag gcactgcaag atgtgcagat ccgcttccag
     2941 ccacagctga accctgatgt ggtggcccca ctgcccaccc acactgccca cgaggacttc
     3001 acatttggag agtctcggcc cgaactgggc tctgagggcc tggggtcagc cgctcacggc
     3061 tcccagcctg acctccgacg aatcgtggag ctgcctgcac ctgccgactt cctcagtctg
     3121 agcagtgaga ccaagcccaa gttgatgaca cctgacgcct tcatgacacc tagcgcctcc
     3181 ttgcagcaga tcactgcctc tcccagcagc agcagcagcg gtagcagcag cagcagcagc
     3241 agtagcagca gctcccttac agctgtgtct gccatgagca gcacctcagc tgtggacccc
     3301 tccttgacca ggccacctga ggagctgacc ttgagcccca agctgcagct ggatggcagc
     3361 ctgacaatga gcagcagtgg cagccttcag gcaagcccgc gtggcctcct gcctggcctg
     3421 ctcccagccc cagctgacaa actgactccc aaggggccgg gccaggtgcc tactgccacc
     3481 tctgcactgt ccctggagct gcaggaagtg gagcccctgg ggctacccca agcctcccct
     3541 agccgcactc gttcccctga tgtcatctcc tcagcttcca ctgccctgtc ccaggacatc
     3601 cctgagattg catctgaggc cctgtcccgt ggttttggct cctctgcacc agagggcctt
     3661 gagccagaca gtatggcttc agccgcctcg gcactgcacc tgctgtcccc acggccccgg
     3721 ccagggcccg agctcggccc ccagctcggg cttgatggag gccctgggga tggagatcgg
     3781 cataataccc cctccctcct ggaggcagcc ttgacccagg aggcctcgac tcctgacagt
     3841 caggtttggc ccacagcacc tgacattact cgtgagacct gcagcaccct ggcagaaagc
     3901 cccaggaatg gccttcagga aaagcacaag agcctggcct tccaccgacc accatatcac
     3961 ctgctgcagc aacgtgacag ccaggatgcc agtgctgagc aaagtgacca tgatgatgag
     4021 gtggccagcc ttgcctctgc ttcaggaggc tttggcacca aagttcctgc tccacggctg
     4081 cctgccaagg actggaagac caagggatcc cctcgaacct cacccaagct caagaggaaa
     4141 agcaagaagg atgatgggga tgcagccatg ggatcccggc tcacagagca ccaggtggca
     4201 gagccccctg aggactggcc agcactaatt tggcaacagc agagagagct ggcagagctg
     4261 cggcacagcc aggaagagct gctgcagcgt ctgtgtaccc aactcgaagg cctgcagagc
     4321 acagtcacag gccacgtaga acgtgccctt gagactcggc acgagcagga acagcggcgg
     4381 ctggagcgag cactggctga ggggcagcag cggggagggc agctgcagga gcagctgaca
     4441 caacagttgt cccaagcact gtcgtcagct gtagctgggc ggctagagcg cagcatacgg
     4501 gatgagatca agaagacagt ccctccatgt gtctcaagga gtctggagcc tatggcaggc
     4561 caactgagca actcagtggc taccaagctc acagctgtgg agggcagcat gaaagagaac
     4621 atctccaagc tgctcaagtc caagaacttg actgatgcca tcgcccgagc agctgcagac
     4681 acattacaag ggccgatgca ggctgcctac cgggaagcct tccagagtgt ggtgctgccg
     4741 gcctttgaga agagctgcca ggccatgttc cagcaaatca atgatagctt ccggctgggg
     4801 acacaggaat acttgcagca gctagaaagc cacatgaaga gccggaaggc acgggaacag
     4861 gaggccaggg agcctgtgct agcccagctg cggggcctgg tcagcacact gcagagtgcc
     4921 actgagcaga tggcagccac cgtggccggc agtgttcgtg ctgaggtgca gcaccagctg
     4981 catgtggctg tgggcagcct gcaggagtcc attttagcac aggtacagcg catcgttaag
     5041 ggtgaggtga gtgtggcgct caaggagcag caggccgccg tcacctccag catcatgcag
     5101 gccatgcgct cagctgctgg cacacctgtc ccctctgccc accttgactg ccaggcccag
     5161 caagcccata tcctgcagct gctgcagcag ggccacctca atcaggcctt ccagcaggcg
     5221 ctgacagctg ctgacctgaa cctggtgctg tatgtgtgtg aaactgtgga cccagcccag
     5281 gtttttgggc agccaccctg cccgctctcc cagcctgtgc tcctttccct catccagcag
     5341 ctggcatctg accttggcac tcgaactgac ctcaagctca gctacctgga agaggccgtg
     5401 atgcacctgg accacagtga ccccatcact cgggaccaca tgggctccgt tatggcccag
     5461 gtgcgccaaa agctttttca gttcctgcag gctgagccac acaactcact tggcaaagca
     5521 gctcggcgtc tcagcctcat gctgcatggc ctcgtgaccc ccagcctccc ttagctgcta
     5581 agcctgcctt gcccaggggt gggatggcac tgaaggccag cagacaggcc taggctgggg
     5641 cagggtcacg gctggccttt acctgctcag gcccccatct ctggggtgtt tgggggtcag
     5701 ggagcaggga gcactggccg tggtctacag cgtgtggtag tcagaaggtt tagctgggcc
     5761 cagggcaggt attgcgcctg cttgggttct gccatgcctg gagcatgacc ctgagatcgt
     5821 gacaccactt gagtggaatt ttccatgttc ctttttacct ctaatttgga tctttttgtt
     5881 tttgaaaaac attgagaaat tcaattaaat gcttttggaa taaaaaagga attctgcagt
     5941 cgacggtacc gcgggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac
     6001 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     6061 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     6121 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6181 tggtttgtcc aaactcatca atgtatctta acgcgtaaat tgtaagcgtt aatattttgt
     6241 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     6301 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     6361 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     6421 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     6481 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6541 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     6601 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     6661 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     6721 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6781 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     6841 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6901 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6961 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     7021 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     7081 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     7141 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     7201 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     7261 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     7321 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     7381 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     7441 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     7501 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     7561 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     7621 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     7681 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     7741 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     7801 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     7861 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     7921 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     7981 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     8041 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     8101 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     8161 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     8221 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     8281 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     8341 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     8401 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     8461 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8521 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     8581 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8641 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8701 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8761 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8821 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8881 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8941 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9001 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9061 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9121 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9181 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     9241 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     9301 t
//
