LOCUS       sequence-401116-        8674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of epithelial cell 
            SARS-CoV2 response..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8674)
  AUTHORS   Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, 
            Serresi M, Cicin-Sain L, Gargiulo G
  TITLE     Pharmacological modulators of epithelial immunity uncovered by 
            synthetic genetic tracing of SARS-CoV-2 infection responses.
  JOURNAL   Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. 
            Epub 2023 Jun 21.
  PUBMED    37343108
REFERENCE   2  (bases 1 to 8674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-23"; volume: "9(25):eadf4975. doi"; pages: " 
            10.1126/sciadv.adf4975. Epub 2023 Jun 21"
COMMENT     Sequence Label: pPB-Neo-COVGT1-d2EGFP-mCherry
FEATURES             Location/Qualifiers
     source          1..8674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1092..1116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1117..1126
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1123..1125,1126..1128,1129..1839,1840..1845,
                     1846..1968)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1168..1189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1429..1448)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1776..1797
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1972..1981
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2123..2141)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             2704..2724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2728..2748
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2752..2772
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2911..2935)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2939..2963)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2967..2991)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3069..3088)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3134..3189
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3188..3207)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        3544..3923
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3924..4127
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4078..4098
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4160..4954
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4160..4954
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4214..4233)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4824..4843
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4992..5009)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4998..5222
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5572..5634
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5700..5719)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5701..5719)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5726..5743)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5726..5742)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5735..5757)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5884..6339
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5971..5990)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6181..6202
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6365..6469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6470..6538,6539..7330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6688..6707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7501..8089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7990..8009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8243..8260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8377..8398
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8413..8418,8419..8436,8437..8443)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8451..8467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8456..8478
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8475..8491
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8475..8491
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8512..8530
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgtgga agcagccctg ggcacctggc
      361 atggacatgc ctgggcagac cccatgccaa atttcatcct gtctcatagc tttccattcc
      421 gtttcttttt cagtttctca ttgttacttc ctgccctgct ctggtttggt gagttttgtc
      481 cttcctttgg ataaaaacaa tgtccccaca cccagtgttg tgtaatcagg gaatgaaaga
      541 atctgctgtg atgccagcac tcaaggggac agtttctgtt gctgtgggaa cctgaacttt
      601 gcatttcctg actttggggt aattaaaatg tcagccttcc agatgctgac gcaacgaggt
      661 gtgggaggga gtcagggggc gtgtccagga ggttggccag gccaatcgga ggcggggcca
      721 gagggttgac tgggcaggcc gggggcgggg ctggaataat aataagagat ggtaggggcg
      781 ggtgtaggct ttgctttatg gtgatgccag gacagttttg ttttccattt ccctgtgcca
      841 taaaacggct ttcttggaaa gctaccagat attccccact aaaagtcttc aaggtcactc
      901 cgtggaaaag ggagggctac ttttgaaggg ggtcactttc cttcagctat gggggcagct
      961 gagccagagg tcaggctgta gtggggaggc cagaaggggc ggggacagcc gggattggga
     1021 ggggagaaca gcagcgtggg cggctggcag aggcagggaa agaccagcag agaggaaaga
     1081 gaaactggga gacaagtttg tacaaaaaag caggctgcca ccatggtgag caagggcgag
     1141 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1201 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1261 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1321 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1381 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1441 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1501 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1561 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1621 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1681 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1741 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1801 gccgccggga tcactctcgg catggacgag ctgtacaaga agcttagcca tggcttcccg
     1861 ccggaggtgg aggagcagga tgatggcacg ctgcccatgt cttgtgccca ggagagcggg
     1921 atggaccgtc accctgcagc ctgtgcttct gctaggatca atgtgtagac tgccaccatg
     1981 gtgagcaagg gcgaggagga caacatggcc atcatcaagg agttcatgag gttcaaggtg
     2041 cacatggagg gcagcgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggcagg
     2101 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc cctgcccttc
     2161 gcctgggaca tcctgagccc ccagttcatg tacggcagca aggcctacgt gaagcacccc
     2221 gccgacatcc ccgactacct gaagctgagc ttccccgagg gcttcaactg ggagagggtg
     2281 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg acagcagcct gcaggacggc
     2341 gagttcatct acaaggtgaa gctgaggggc accaacttcc ccagcgacgg ccccgtgatg
     2401 cagtgcagga ccatgggctg ggaggccagc accgagagga tgtaccccga ggacggcgcc
     2461 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgccgag
     2521 gtgaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtggac
     2581 atcaagctgg acatcctgag ccacaacgag gactacacca tcgtggagca gtacgagagg
     2641 gccgagggca ggcacagcac cggcggcatg gacgagctgt acaagctcag atctcgagct
     2701 gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga tccaaaaaag
     2761 aagagaaagg taggatccac cggatctagc catggcttcc cgccggaggt ggaggagcag
     2821 gatgatggca cgctgcccat gtcttgtgcc caggagagcg ggatggaccg tcaccctgca
     2881 gcctgtgctt ctgctaggat caatgtgtag acccagcttt cttgtacaaa gtggtgatac
     2941 ccagctttct tgtacaaagt ggtgataccc agctttcttg tacaaagtgg tgatcctcag
     3001 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     3061 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     3121 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     3181 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     3241 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta
     3301 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa
     3361 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca
     3421 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc
     3481 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttcgc
     3541 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     3601 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3661 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     3721 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     3781 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     3841 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     3901 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     3961 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4021 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4081 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
     4141 gagaacccac tgcgccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4201 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4261 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4321 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4381 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4441 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4501 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4561 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4621 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4681 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4741 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4801 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4861 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4921 cgccttctat cgccttcttg acgagttctt ctgactcgag tctagagggc ccgtttaaac
     4981 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5041 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5101 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5161 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5221 ggctcgagtt aattaacgag agcataatat tgatatgtgc caaagttgtt tctgactgac
     5281 taataagtat aatttgtttc tattatgtat aggttaagct aattacttat tttataatac
     5341 aacatgactg tttttaaagt acaaaataag tttatttttg taaaagagag aatgtttaaa
     5401 agttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa
     5461 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt
     5521 aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca
     5581 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaaat
     5641 aatagtttct aattttttta ttattcagcc tgctgtcgtg aataccgagc tccaattcgc
     5701 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     5761 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     5821 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     5881 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     5941 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6001 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6061 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6121 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6181 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6241 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6301 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     6361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6481 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6541 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6601 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6661 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6721 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6781 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6841 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6901 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6961 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7021 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7081 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7141 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7201 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7261 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7321 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7381 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7621 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8101 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8161 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8221 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8281 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     8341 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     8401 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     8461 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     8521 tcactaaagg gaacaaaagc tggtacctcg cgcgacttgg tttgccattc tttagcgcgc
     8581 gtcgcgtcac acagcttggc cacaatgtgg tttttgtcaa acgaagattc tatgacgtgt
     8641 ttaaagttta ggtcgagtaa agcgcaaatc tttt
//
