LOCUS       sequence-401120-        8673 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of epithelial cell 
            SARS-CoV2 response..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8673)
  AUTHORS   Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, 
            Serresi M, Cicin-Sain L, Gargiulo G
  TITLE     Pharmacological modulators of epithelial immunity uncovered by 
            synthetic genetic tracing of SARS-CoV-2 infection responses.
  JOURNAL   Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. 
            Epub 2023 Jun 21.
  PUBMED    37343108
REFERENCE   2  (bases 1 to 8673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-23"; volume: "9(25):eadf4975. doi"; pages: " 
            10.1126/sciadv.adf4975. Epub 2023 Jun 21"
COMMENT     Sequence Label: pPB-Neo-COVGT4-d2EGFP-mCherry
FEATURES             Location/Qualifiers
     source          1..8673
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..39
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    316..336
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1091..1115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1116..1125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1122..1124,1125..1127,1128..1838,1839..1844,
                     1845..1967)
                     /codon_start=1
                     /product="EGFP destabilized by fusion to residues 422-461 
                     of mouse ornithine decarboxylase, giving an in vivo 
                     half-life of ~2 hours"
                     /label=d2EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKKLSHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV
                     "
     primer_bind     complement(1167..1188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1428..1447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1775..1796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      1971..1980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2122..2140)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             2703..2723
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2727..2747
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2751..2771
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2910..2934)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2938..2962)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2966..2990)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3068..3087)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3133..3188
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3187..3206)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        3543..3922
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3923..4126
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4077..4097
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4159..4953
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4159..4953
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4213..4232)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4823..4842
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4991..5008)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4997..5221
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   5571..5633
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(5699..5718)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5700..5718)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5725..5742)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5725..5741)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5734..5756)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5883..6338
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5970..5989)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6180..6201
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6364..6468
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6469..6537,6538..7329)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6687..6706)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7500..8088
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7989..8008
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8242..8259
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8376..8397
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8412..8417,8418..8435,8436..8442)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8450..8466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8455..8477
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8474..8490
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8474..8490
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8511..8529
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
       61 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
      121 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
      181 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
      241 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
      301 tgttatagat atcatcaact ttgtatagaa aagttgggca ggagaattgc ttgaacccag
      361 gaggcggagg tagcggtgag ctgagattgc accactgcac tccagcctgg gcaataaaag
      421 tgaaactcca tctcaaaaac aaccacacac acagagaaga gaaaagagaa aagaataaga
      481 aagaatggga agcaaggaga tgaaggagag gaagtcgtag ctgggaaggg gaccaggaag
      541 tgggtgtcag gtccaagagc tgctagaaag aaacgaaact gaaagcaggg aatttcccaa
      601 gtttggggaa gacaggaact gcagcgcccc tccccgttgg aacatttgct ctgaattaag
      661 aagaaatgtt gttatttaca tctgacctat tttctctaga cctcagtggt ttccaggaca
      721 acacactgag gttttgaagg aggggtgcaa ggggaagtgc tgactcatct tttttgccac
      781 tcccttccca ccctgtcccc agaggttttt ctgtcccttt atgtctatga acaagtacag
      841 ctgaggccat cagcactgca cacacctgga acagtcttgt taaaacagga cctccctaaa
      901 ggccaagagc taaggaaaga gaaagtgaag gactgagcag gcagctgagc cagaggtcag
      961 gctgtagtgg ggaggccaga aggggcgggg acagccggga ttgggagggg agaacagcag
     1021 cgtgggcggc tggcagaggc agggaaagac cagcagagag gaaagagaaa ctgggagagg
     1081 gaggaaggga gcaagtttgt acaaaaaagc aggctgccac catggtgagc aagggcgagg
     1141 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1201 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1261 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     1321 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1381 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1441 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1501 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1561 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1621 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1681 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1741 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1801 ccgccgggat cactctcggc atggacgagc tgtacaagaa gcttagccat ggcttcccgc
     1861 cggaggtgga ggagcaggat gatggcacgc tgcccatgtc ttgtgcccag gagagcggga
     1921 tggaccgtca ccctgcagcc tgtgcttctg ctaggatcaa tgtgtagact gccaccatgg
     1981 tgagcaaggg cgaggaggac aacatggcca tcatcaagga gttcatgagg ttcaaggtgc
     2041 acatggaggg cagcgtgaac ggccacgagt tcgagatcga gggcgagggc gagggcaggc
     2101 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     2161 cctgggacat cctgagcccc cagttcatgt acggcagcaa ggcctacgtg aagcaccccg
     2221 ccgacatccc cgactacctg aagctgagct tccccgaggg cttcaactgg gagagggtga
     2281 tgaacttcga ggacggcggc gtggtgaccg tgacccagga cagcagcctg caggacggcg
     2341 agttcatcta caaggtgaag ctgaggggca ccaacttccc cagcgacggc cccgtgatgc
     2401 agtgcaggac catgggctgg gaggccagca ccgagaggat gtaccccgag gacggcgccc
     2461 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgccgagg
     2521 tgaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtggaca
     2581 tcaagctgga catcctgagc cacaacgagg actacaccat cgtggagcag tacgagaggg
     2641 ccgagggcag gcacagcacc ggcggcatgg acgagctgta caagctcaga tctcgagctg
     2701 atccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtagat ccaaaaaaga
     2761 agagaaaggt aggatccacc ggatctagcc atggcttccc gccggaggtg gaggagcagg
     2821 atgatggcac gctgcccatg tcttgtgccc aggagagcgg gatggaccgt caccctgcag
     2881 cctgtgcttc tgctaggatc aatgtgtaga cccagctttc ttgtacaaag tggtgatacc
     2941 cagctttctt gtacaaagtg gtgataccca gctttcttgt acaaagtggt gatcctcagg
     3001 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     3061 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     3121 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3181 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     3241 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     3301 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     3361 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     3421 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     3481 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttcgcg
     3541 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     3601 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     3661 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     3721 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     3781 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     3841 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     3901 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     3961 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     4021 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     4081 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     4141 agaacccact gcgccaccat gattgaacaa gatggattgc acgcaggttc tccggccgct
     4201 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     4261 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     4321 ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc
     4381 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     4441 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     4501 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     4561 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     4621 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     4681 aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     4741 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     4801 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     4861 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     4921 gccttctatc gccttcttga cgagttcttc tgactcgagt ctagagggcc cgtttaaacc
     4981 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     5041 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     5101 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     5161 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     5221 gctcgagtta attaacgaga gcataatatt gatatgtgcc aaagttgttt ctgactgact
     5281 aataagtata atttgtttct attatgtata ggttaagcta attacttatt ttataataca
     5341 acatgactgt ttttaaagta caaaataagt ttatttttgt aaaagagaga atgtttaaaa
     5401 gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa ataaataaac
     5461 ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata ataaaactta
     5521 atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca catgcgtcaa
     5581 ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta gggttaaata
     5641 atagtttcta atttttttat tattcagcct gctgtcgtga ataccgagct ccaattcgcc
     5701 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
     5761 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     5821 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     5881 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     5941 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6001 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6061 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6121 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6181 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     6241 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     6301 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     6361 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6421 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6481 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6541 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6601 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6661 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     6721 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6781 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6841 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6901 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6961 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     7021 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     7081 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     7141 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     7201 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7261 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7321 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7381 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7441 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7501 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7561 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7621 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     7681 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7741 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7801 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7861 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7921 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7981 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8041 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8101 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8161 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8221 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     8281 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     8341 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     8401 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     8461 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcga aattaaccct
     8521 cactaaaggg aacaaaagct ggtacctcgc gcgacttggt ttgccattct ttagcgcgcg
     8581 tcgcgtcaca cagcttggcc acaatgtggt ttttgtcaaa cgaagattct atgacgtgtt
     8641 taaagtttag gtcgagtaaa gcgcaaatct ttt
//
