LOCUS       sequence-401121-       10471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for genetic tracing of epithelial cell 
            SARS-CoV2 response..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Jiang B, Schmitt MJ, Rand U, Company C, Dramaretska Y, Grossmann M, 
            Serresi M, Cicin-Sain L, Gargiulo G
  TITLE     Pharmacological modulators of epithelial immunity uncovered by 
            synthetic genetic tracing of SARS-CoV-2 infection responses.
  JOURNAL   Sci Adv. 2023 Jun 23;9(25):eadf4975. doi: 10.1126/sciadv.adf4975. 
            Epub 2023 Jun 21.
  PUBMED    37343108
REFERENCE   2  (bases 1 to 10471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-06-23"; volume: "9(25):eadf4975. doi"; pages: " 
            10.1126/sciadv.adf4975. Epub 2023 Jun 21"
COMMENT     Sequence Label: pBA407-Neo-COVGT5-mCherry
FEATURES             Location/Qualifiers
     source          1..10471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3724..3748
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3749..3758
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3779..4489
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3924..3942)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4192..4211
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4521..4547
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        4633..5844
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5681..5700
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5890..6684
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5890..6684
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5944..5963)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6554..6573
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    6861..7449
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6914..6934)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7332..7343)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7524..7757
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7876..7894)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7962..8066
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8067..8135,8136..8927)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8285..8304)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9098..9686
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9587..9606
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9840..9857
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10097..10118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10133..10138,10139..10156,10157..10163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10171..10187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10176..10198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10195..10211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10195..10211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10261..10280
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 agcagccggc aggagaattg cttgaaccca ggaggcggag gtagcggtga gctgagattg
     2281 caccactgca ctccagcctg ggcaataaaa gtgaaactcc atctcaaaaa caaccacaca
     2341 cacagagaag agaaaagaga aaagaataag aaagaatggg aagcaaggag atgaaggaga
     2401 ggaagtcgta gctgggaagg ggaccaggaa gtgggtgtca ggtccaagag ctgctagaaa
     2461 gaaacgaaac tgaaagcagg gaatttccca agtttgggga agacaggaac tgcagcgccc
     2521 ctccccgttg gaacatttgc tctgaattaa gaagaaatgt tgttatttac atctgaccta
     2581 ttttctctag acctcagtgg tttccaggac aacacactga ggttttgaag gaggggtgca
     2641 aggggaagtg ctgactcatc ttttttgcca ctcccttccc accctgtccc cagaggtttt
     2701 tctgtccctt tatgtctatg aacaagtaca gctgaggcca tcagcactgc acacacctgg
     2761 aacagtcttg ttaaaacagg acctccctaa aggccaagag ctaaggaaag agaaagtgaa
     2821 ggactgagca ggcagctgag ccagaggtca ggctgtagtg gggaggccag aaggggcggg
     2881 gacagccggg attgggaggg gagaacagca gcgtgggcgg ctggcagagg cagggaaaga
     2941 ccagcagaga ggaaagagaa actgggagag ggaggaaggg agggcacctg gcatggacat
     3001 gcctgggcag accccatgcc aaatttcatc ctgtctcata gctttccatt ccgtttcttt
     3061 ttcagtttct cattgttact tcctgccctg ctctggtttg gtgagttttg tccttccttt
     3121 ggataaaaac aatgtcccca cacccagtgt tgtgtaatca gggaatgaaa gaatctgctg
     3181 tgatgccagc actcaagggg acagtttctg ttgctgtggg aacctgaact ttgcatttcc
     3241 tgactttggg gtaattaaaa tgtcagcctt ccagatgctg acgcaacgag gtgtgggagg
     3301 gagtcagggg gcgtgtccag gaggttggcc aggccaatcg gaggcggggc cagagggttg
     3361 actgggcagg ccgggggcgg ggctggaata ataataagag atggtagggg cgggtgtagg
     3421 ctttgcttta tggtgatgcc aggacagttt tgttttccat ttccctgtgc cataaaacgg
     3481 ctttcttgga aagctaccag atattcccca ctaaaagtct tcaaggtcac tccgtggaaa
     3541 agggagggct acttttgaag ggggtcactt tccttcagct atgggggcag ctgagccaga
     3601 ggtcaggctg tagtggggag gccagaaggg gcggggacag ccgggattgg gaggggagaa
     3661 cagcagcgtg ggcggctggc agaggcaggg aaagaccagc agagaggaaa gagaaactgg
     3721 gagacaagtt tgtacaaaaa agcaggctgc caccatggtg agcaagggcg aggagaacat
     3781 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     3841 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     3901 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     3961 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     4021 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     4081 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     4141 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     4201 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     4261 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     4321 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     4381 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     4441 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaaa acctcgagtc
     4501 tagagggccc gcggttcgaa tacccatacg acgtcccaga ctacgcttag taatgattaa
     4561 ttaaactaga aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagtaacc
     4621 cacccaagat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca
     4681 cggcgagcgc tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga
     4741 cgctcaggac agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga
     4801 aggacatttt aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg
     4861 gaacaggcga ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg
     4921 gtgaacgccg atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc
     4981 cgggatttgg gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg
     5041 ggctgctggg ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg
     5101 tggagagacc gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg
     5161 ttggggggag cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga
     5221 ggcgggctgt gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg
     5281 tcttgaggcc ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca
     5341 tctggggacc ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc
     5401 gggggcggca gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc
     5461 cctcgtcgtg tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg
     5521 ccggtaggtg tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag
     5581 gctctcctga atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag
     5641 tttctttggt cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact
     5701 atgcgctcgg ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa
     5761 tcatttgggt caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg
     5821 ccgtttttgg cttttttgtt agacgaagct tggtaccgag ctcggatctc caccccgtac
     5881 cggtccacca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5941 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     6001 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     6061 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     6121 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     6181 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     6241 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     6301 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     6361 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
     6421 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6481 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6541 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6601 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6661 cgccttcttg acgagttctt ctgatgtaca agtaaagcgg ccgcgactct agatcataat
     6721 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     6781 gaacctgaaa cataaaatga atgcaattgt tgttgtttag tccctcccaa ttcgatatca
     6841 agcttatcgc gatagatcct aatcaacctc tggattacaa aatttgtgaa agattgactg
     6901 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6961 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     7021 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7081 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7141 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7201 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     7261 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7321 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7381 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7441 cctccccgcc tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca
     7501 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     7561 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7621 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7681 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7741 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7801 aaagaaatga atatcagaga gtgagaggcc cgggttaatt aaggaaaggg ctagatcatt
     7861 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7921 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7981 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8041 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8101 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8161 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8221 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8281 ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc
     8341 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8401 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     8461 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     8521 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     8581 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     8641 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     8701 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     8761 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     8821 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     8881 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8941 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9001 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9061 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9121 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9181 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9241 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9301 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9361 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9421 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     9481 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     9541 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     9601 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     9661 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     9721 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     9781 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     9841 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9901 gttggccgat tcattaatgc agcaagctca tggctgacta atttttttta tttatgcaga
     9961 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10021 cctaggcttt tgcaaaaagc tccccgtggc acgacaggtt tcccgactgg aaagcgggca
    10081 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
    10141 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10201 acagctatga catgattacg aatttcacaa ataaagcatt tttttcactg cattctagtt
    10261 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
    10321 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
    10381 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
    10441 tttgttaaat atgtactaca aacttagtag t
//
