LOCUS       sequence-401122-       13161 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human cis-prenyltransferase complex for purification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13161)
  AUTHORS   Grabinska KA, Edani BH, Park EJ, Kraehling JR, Sessa WC
  TITLE     A conserved C-terminal RXG motif in the NgBR subunit of 
            cis-prenyltransferase is critical for prenyltransferase activity.
  JOURNAL   J Biol Chem. 2017 Oct 20;292(42):17351-17361. doi: 
            10.1074/jbc.M117.806034. Epub 2017 Aug 23.
  PUBMED    28842490
REFERENCE   2  (bases 1 to 13161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M117.806034"; journalName: "J Biol Chem"; date: 
            "2017-10-20- 20"; volume: "292"; issue: "42"; pages: "17351-17361"
COMMENT     Sequence Label: pKGmDUET-NgBR/hCIT
FEATURES             Location/Qualifiers
     source          1..13161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             719..736
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     intron          1598..1827
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1810..1829
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    1885..2458
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2052..2069)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2279..2298
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2504..2527
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2564..2587
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             2594..2614
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    3658..3779
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3669..3688)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3723..3742
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3922..3944)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4504..6293
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(8955..8973)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9041..9145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9146..9214,9215..10006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9364..9383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10177..10765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10666..10685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        11196..11341
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             11384..12421
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(12419..12438)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12463..12511
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gatccatgac ggggctgtac gagctggtgt ggcgggtgct
      661 gcacgcgctg ctctgtctgc accgcacgct cacctcctgg ctccgcgttc ggttcggcca
      721 tcaccatcat caccacggca cctggaactg gatctggcgg cgctgctgcc gcgccgcctc
      781 tgccgcggtc ctagcgccgc tcggcttcac gctccgcaag cccccggcag tcggcaggaa
      841 ccgccgtcac caccggcacc cgcgcggggg gtcgtgcctg gcagccgcac accaccggat
      901 gcgctggcgc gcggacggtc gttccttgga gaagctgcct gtgcatatgg gcctggtgat
      961 caccgaggtg gagcaggaac ccagcttctc ggacatcgcg agcctcgtgg tgtggtgtat
     1021 ggccgtgggc atctcctaca ttagcgtcta cgaccaccaa ggtattttca aaagaaataa
     1081 ttccagactg atggatgaaa ttttaaaaca acagcaagaa cttctgggcc tagattgttc
     1141 aaaatactca ccagaatttg caaatagtaa tgacaaagat gatcaagttt taaattgcca
     1201 tttggcagtg aaggtgctgt ctccggaaga tggaaaagca gatattgtaa gagctgctca
     1261 ggacttttgc cagttagtag cccagaagca aaagagaccc acagatttgg atgtagatac
     1321 gttagccagt ttacttagtt caaatggttg tcctgatcct gatttagtat tgaagttcgg
     1381 tcctgtggac agcacattag gctttcttcc ctggcacatc agattgactg agattgtctc
     1441 tttgccttcc cacctaaaca tcagttatga ggactttttc tctgcccttc gtcaatatgc
     1501 agcctgtgaa cagcgtctgg gaaagtaggc ggccgcacta gtaacggccg ccagtgtgct
     1561 ggaattaatt cgctgtctgc gagggccagc tgttggggtg agtactccct ctcaaaagcg
     1621 ggcatgactt ctgcgctaag attgtcagtt tccaaaaacg aggaggattt gatattcacc
     1681 tggcccgcgg tgatgccttt gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt
     1741 ttgttgtcaa gcttgaggtg tggcaggctt gagatctggc catacacttg agtgacaatg
     1801 acatccactt tgcctttctc tccacaggtg tccactccca ggtccaactg caggtcgagc
     1861 atgcatctag ggcggccaat tccgcccctc tcccyccccc ccccctaacg ttactggccg
     1921 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     1981 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     2041 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     2101 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     2161 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     2221 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     2281 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     2341 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     2401 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc
     2461 ttgccacaac cccgggctcg agcagctgtt tgggatgagc gcttggagcc acccgcagtt
     2521 cgagaaaggt ggaggttccg gaggtggatc gggaggtgga tcgtggagcc acccgcagtt
     2581 cgaaaaaggc gccgagaatt tgtattttca gggtgagctc atgtcatgga tcaaggaagg
     2641 agagctgtca ctttgggagc ggttctgtgc caacatcata aaggcaggcc caatgccgaa
     2701 acacattgca ttcataatgg acgggaaccg tcgctatgcc aagaagtgcc aggtggagcg
     2761 gcaggaaggc cactcacagg gcttcaacaa gctagctgag actctgcggt ggtgtttgaa
     2821 cctgggcatc ctagaggtga cagtctacgc attcagcatt gagaacttca aacgctccaa
     2881 gagtgaggta gacgggctta tggatctggc ccggcagaag ttcagccgct tgatggaaga
     2941 aaaggagaaa ctgcagaagc atggggtgtg tatccgggtc ctgggcgatc tgcacttgtt
     3001 gcccttggat ctccaggagc tgattgcaca agctgtacag gccacgaaga actacaacaa
     3061 gtgtttcctg aatgtctgtt ttgcatacac atcccgtcat gagatcagca atgctgtgag
     3121 agagatggcc tggggggtgg agcaaggcct gttggatccc agtgatatct ctgagtctct
     3181 gcttgataag tgcctctata ccaaccgctc tcctcatcct gacatcttga tacggacttc
     3241 tggagaagtg cggctgagtg acttcttgct atggcagacc tctcactcct gcctggtgtt
     3301 ccaacccgtt ctgtggccag agtatacatt ttggaacctc ttcgaggcca tcctgcagtt
     3361 ccagatgaac catagcatgc ttcagaaggc ccgagacatg tatgcagagg agcggaagag
     3421 gcagcagctg gagagggacc aggctacagt gacagagcag ctgctgcgag aggggctcca
     3481 agccagtggg gacgcccagc tccgaaggac acgcttgcac aaactctcgg ccagacggga
     3541 agagcgagtc caaggcttcc tgcaggcctt ggaactcaag cgagctgact ggctggcccg
     3601 tctgggcact gcatcagcct gaggtaccaa gggcgaatta aacgctagca gacatgataa
     3661 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3721 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3781 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     3841 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatccggct gcctcgcgcg
     3901 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     3961 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     4021 gtgtcggggc gcagccatga ggtcgactct agaggatcga tccccgcccc ggacgaacta
     4081 aacctgacta cgacatctct gccccttctt cgcggggcag tgcatgtaat cccttcagtt
     4141 ggttggtaca acttgccaac tgggccctgt tccacatgtg acacgggggg ggaccaaaca
     4201 caaaggggtt ctctgactgt agttgacatc cttataaatg gatgtgcaca tttgccaaca
     4261 ctgagtggct ttcatcctgg agcagacttt gcagtctgtg gactgcaaca caacattgcc
     4321 tttatgtgta actcttggct gaagctctta caccaatgct gggggacatg tacctcccag
     4381 gggcccagga agactacggg aggctacacc aacgtcaatc agaggggcct gtgtagctac
     4441 cgataagcgg accctcaaga gggcattagc aatagtgttt ataaggcccc cttgttaacc
     4501 ctaaacgggt agcatatgct tcccgggtag tagtatatac tatccagact aaccctaatt
     4561 caatagcata tgttacccaa cgggaagcat atgctatcga attagggtta gtaaaagggt
     4621 cctaaggaac agcgatatct cccaccccat gagctgtcac ggttttattt acatggggtc
     4681 aggattccac gagggtagtg aaccatttta gtcacaaggg cagtggctga agatcaagga
     4741 gcgggcagtg aactctcctg aatcttcgcc tgcttcttca ttctccttcg tttagctaat
     4801 agaataactg ctgagttgtg aacagtaagg tgtatgtgag gtgctcgaaa acaaggtttc
     4861 aggtgacgcc cccagaataa aatttggacg gggggttcag tggtggcatt gtgctatgac
     4921 accaatataa ccctcacaaa ccccttgggc aataaatact agtgtaggaa tgaaacattc
     4981 tgaatatctt taacaataga aatccatggg gtggggacaa gccgtaaaga ctggatgtcc
     5041 atctcacacg aatttatggc tatgggcaac acataatcct agtgcaatat gatactgggg
     5101 ttattaagat gtgtcccagg cagggaccaa gacaggtgaa ccatgttgtt acactctatt
     5161 tgtaacaagg ggaaagagag tggacgccga cagcagcgga ctccactggt tgtctctaac
     5221 acccccgaaa attaaacggg gctccacgcc aatggggccc ataaacaaag acaagtggcc
     5281 actctttttt ttgaaattgt ggagtggggg cacgcgtcag cccccacacg ccgccctgcg
     5341 gttttggact gtaaaataag ggtgtaataa cttggctgat tgtaaccccg ctaaccactg
     5401 cggtcaaacc acttgcccac aaaaccacta atggcacccc ggggaatacc tgcataagta
     5461 ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg gaggacaaat tacacacact
     5521 tgcgcctgag cgccaagcac agggttgttg gtcctcatat tcacgaggtc gctgagagca
     5581 cggtgggcta atgttgccat gggtagcata tactacccaa atatctggat agcatatgct
     5641 atcctaatct atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     5701 atcctaatct atatctgggt agtatatgct atcctaattt atatctgggt agcataggct
     5761 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     5821 atcctaatct gtatccgggt agcatatgct atcctaatag agattagggt agtatatgct
     5881 atcctaattt atatctgggt agcatatact acccaaatat ctggatagca tatgctatcc
     5941 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagca taggctatcc
     6001 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     6061 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     6121 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     6181 tcatgcatat acagtcagca tatgataccc agtagtagag tgggagtgct atcctttgca
     6241 tatgccgcca cctcccaagg gggcgtgaat tttcgctgct tgtccttttc ctgctggttg
     6301 ctcccattct taggtgaatt taaggaggcc aggctaaagc cgtcgcatgt ctgattgctc
     6361 accaggtaaa tgtcgctaat gttttccaac gcgagaaggt gttgagcgcg gagctgagtg
     6421 acgtgacaac atgggtatgc ccaattgccc catgttggga ggacgaaaat ggtgacaaga
     6481 cagatggcca gaaatacacc aacagcacgc atgatgtcta ctggggattt attctttagt
     6541 gcgggggaat acacggcttt taatacgatt gagggcgtct cctaacaagt tacatcactc
     6601 ctgcccttcc tcaccctcat ctccatcacc tccttcatct ccgtcatctc cgtcatcacc
     6661 ctccgcggca gccccttcca ccataggtgg aaaccaggga ggcaaatcta ctccatcgtc
     6721 aaagctgcac acagtcaccc tgatattgca ggtaggagcg ggctttgtca taacaaggtc
     6781 cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt tgtaaaaaga ccatgaaata
     6841 acagacaatg gactccctta gcgggccagg ttgtgggccg ggtccagggg ccattccaaa
     6901 ggggagacga ctcaatggtg taagacgaca ttgtggaata gcaagggcag ttcctcgcct
     6961 taggttgtaa agggaggtct tactacctcc atatacgaac acaccggcga cccaagttcc
     7021 ttcgtcggta gtcctttcta cgtgactcct agccaggaga gctcttaaac cttctgcaat
     7081 gttctcaaat ttcgggttgg aacctccttg accacgatgc tttccaaacc accctccttt
     7141 tttgcgcctg cctccatcac cctgaccccg gggtccagtg cttgggcctt ctcctgggtc
     7201 atctgcgggg ccctgctcta tcgctcccgg gggcacgtca ggctcaccat ctgggccacc
     7261 ttcttggtgg tattcaaaat aatcggcttc ccctacaggg tggaaaaatg gccttctacc
     7321 tggagggggc ctgcgcggtg gagacccgga tgatgatgac tgactactgg gactcctggg
     7381 cctcttttct ccacgtccac gacctctccc cctggctctt tcacgacttc cccccctggc
     7441 tctttcacgt cctctacccc ggcggcctcc actacctcct cgaccccggc ctccactacc
     7501 tcctcgaccc cggcctccac tgcctcctcg accccggcct ccacctcctg ctcctgcccc
     7561 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgctc ctgcccctcc
     7621 tscccctcct gctcctsccc ctcctgcccc tcctsctcct gcccctcctg cccctcctcc
     7681 tsctcctgcc cctcctsccc ctcctccysc tcctsccccy cctccccctc ctscycctsc
     7741 ccctcctscc ccycctscyc ctscccctcc tscccctcct scycctsccc ctcctsctcc
     7801 tcccccyccy ccyccycccc cyccyccycc tcccccyccy cccccyccyc ccccyccycc
     7861 ycctcctccc cctcctcctc ctccycctcc tscccctcct scccctccts ctcctscccc
     7921 tcctcctsct cctscccctc ctscccctcc tscccctcct cctsctccts cccctcctsc
     7981 ccctcctcct sctcctsccc ctcctcctsc tcctscccct cctgcccctc ctgcccctcc
     8041 tcctsctcct scccctccts cccctcctcc tsctcctscc cctcctccys ctcctscccc
     8101 tcctscycct cctscycctc ctcctsctcc tscccctcct cctsctccts cccctcctgc
     8161 ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc ctgcccctcc
     8221 tgctcctgcc cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc
     8281 aatgcaactt ggacgttttt ggggtctccg gacaccatct ctatgtcttg gccctgatcc
     8341 tgagccgccc ggggctcctg gtcttccgcc tcctcgtcct cgtcctcttc cccgtcctcg
     8401 tccatggtta tcaccccctc ttctttgagg tccactgccg ccggagcctt ctggtccaga
     8461 tgtgtctccc ttctctccta ggccatttcc aggtcctgta cctggcccct cgtcagacat
     8521 gattcacact aaaagagatc aatagacatc tttattagac gacgctcagt gaatacaggg
     8581 agtgcagact cctgccccct ccaacagccc ccccaccctc atccccttca tggtcgctgt
     8641 cagacagatc caggtctgaa aattccccat cctccgaacc atcctcgtcc tcatcaccaa
     8701 ttactcgcag cccggaaaac tcccgctgaa catcctcaag atttgcgtcc tgagcctcaa
     8761 gccaggcctc aaattcctcg tccccctttt tgctggacgg tagggatggg gattctcggg
     8821 acccctcctc ttcctcttca aggtcaccag acagagatgc tactggggca acggaagaaa
     8881 agctgggtgc ggcctgtgag gatcagctta tcgatgataa gctgtcaaac atgagaattc
     8941 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     9001 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     9061 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     9121 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     9181 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     9241 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     9301 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     9361 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg
     9421 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     9481 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     9541 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     9601 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     9661 aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt
     9721 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     9781 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     9841 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     9901 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     9961 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    10021 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    10081 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    10141 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    10201 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    10261 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    10321 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    10381 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    10441 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
    10501 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
    10561 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
    10621 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
    10681 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
    10741 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
    10801 cttttgctgg ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
    10861 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
    10921 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccc cgagatgcgc
    10981 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc
    11041 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
    11101 gcgaggtgcc gccctgcttc atccccgtgg cccgttgctc gcgtttgctg gcggtgtccc
    11161 cggaagaaat atatttgcat gtctttagtt ctatgatgac acaaaccccg cccagcgtct
    11221 tgtcattggc gaattcgaac acgcagatgc agtcggggcg gcgcggtccg aggtccactt
    11281 cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg accctgcagc gacccgctta
    11341 acagcgtcaa cagcgtgccg cagatcccgg ggggcaatga gatatgaaaa agcctgaact
    11401 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
    11461 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
    11521 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
    11581 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
    11641 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
    11701 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
    11761 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
    11821 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
    11881 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
    11941 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
    12001 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
    12061 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
    12121 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
    12181 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
    12241 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
    12301 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
    12361 cgatagtgga aaccgacgcc ccagcactcg tccggatcgg gagatggggg aggctaactg
    12421 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
    12481 ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
    12541 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
    12601 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
    12661 gcaggccctg ccatagccac tggccccgtg ggttagggac ggggtccccc atggggaatg
    12721 gtttatggtt cgtgggggtt attattttgg gcgttgcgtg gggtcaggtc cacgactgga
    12781 ctgagcagac agacccatgg tttttggatg gcctgggcat ggaccgcatg tactggcgcg
    12841 acacgaacac cgggcgtctg tggctgccaa acacccccga cccccaaaaa ccaccgcgcg
    12901 gatttctggc gtgccaagct agtcgaccaa ttctcatgtt tgacagctta tcatcgcaga
    12961 tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg aagatctata
    13021 cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata
    13081 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
    13141 catgtccaat atgaccgcca t
//
