LOCUS       sequence-401124-       10780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  plasmid for expressing the DNMT3bCI (catalytic inactive).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10780)
  AUTHORS   Ma Y, Budde MW, Zhu J, Elowitz MB
  TITLE     Tuning Methylation-Dependent Silencing Dynamics by Synthetic 
            Modulation of CpG Density.
  JOURNAL   ACS Synth Biol. 2023 Aug 12. doi: 10.1021/acssynbio.3c00078.
  PUBMED    37572041
REFERENCE   2  (bases 1 to 10780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Aug 12. doi: 10.1021/acssynbio.3c00078."
COMMENT     Sequence Label: vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI
FEATURES             Location/Qualifiers
     source          1..10780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(171..962,963..1031))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     794..813
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1032..1136)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1162..1617)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1299..1320)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1511..1530
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1744..1766
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1758..1775
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1759..1775
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   1905..1967
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     intron          2644..3582
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3539..3559
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3615..3633
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             3694..4071
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             4078..4785
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4223..4241)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4491..4510
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4789..4842
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(6927..6946)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6928..6946)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    7220..7341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7413..7433)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7418..7747
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7598..7733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7660..7679
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7795..7842
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7861..8259
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(8319..8339)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8381..8502
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   9265..9299
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9790..9806)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9790..9806)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9803..9825)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9814..9830
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9838..9844,9845..9862,9863..9868))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9883..9904
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10021..10038)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10192..10780)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10272..10291)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccaga attctaacta
     2401 taacggtcct aaggtagcga aggtctcgcg tactgtgagc gcacatcgcc cacagtcccc
     2461 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     2521 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2581 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2641 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     2701 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     2761 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     2821 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     2881 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     2941 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     3001 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     3061 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     3121 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     3181 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     3241 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     3301 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     3361 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     3421 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     3481 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     3541 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg agctagcgat
     3601 tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggagct ccaccgcggt
     3661 ggcggccgct ctagcccggg cggatccgcc accatgccag agccagcgaa gtctgctccc
     3721 gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg cggcaagaag
     3781 cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct gaagcaggtc
     3841 caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac
     3901 attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc
     3961 atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga gttggccaag
     4021 cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa gcctaggatg
     4081 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     4141 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     4201 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     4261 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     4321 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     4381 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     4441 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     4501 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     4561 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     4621 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     4681 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     4741 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtctga gggcaggggg
     4801 agcctcctaa cttgcggaga cgtcgaggag aatcctggcc catctagact ggacaagagc
     4861 aaagtcataa acggagctct ggaattactc aatggtgtcg gtatcgaagg cctgacgaca
     4921 aggaaactcg ctcaaaagct gggagttgag cagcctaccc tgtactggca cgtgaagaac
     4981 aagcgggccc tgctcgatgc cctgccaatc gagatgctgg acaggcatca tacccacttc
     5041 tgccccctgg aaggcgagtc atggcaagac tttctgcgga acaacgccaa gtcataccgc
     5101 tgtgctctcc tctcacatcg cgacggggct aaagtgcatc tcggcacccg cccaacagag
     5161 aaacagtacg aaaccctgga aaatcagctc gcgttcctgt gtcagcaagg cttctccctg
     5221 gagaacgcac tgtacgctct gtccgccgtg ggccacttta cactgggctg cgtattggag
     5281 gaacaggagc atcaagtagc aaaagaggaa agagagacac ctaccaccga ttctatgccc
     5341 ccacttctga gacaagcaat tgagctgttc gaccggcagg gagccgaacc tgccttcctt
     5401 ttcggcctgg aactaatcat atgtggcctg gagaaacagc taaagtgcga aagcggcggg
     5461 ggtagcggca gcggtagcgc atgcacaacc aatgactctg ctgcttctga gtccccccca
     5521 cccaagcgcc tcaagacaaa tagctatggc gggaaggacc gaggggagga tgaggagagc
     5581 cgagaacgga tggcttctga agtcaccaac aacaagggca atctggaaga ccgctgtttg
     5641 tcctgtggaa agaagaaccc tgtgtccttc caccccctct ttgagggtgg gctctgtcag
     5701 agttgccggg atcgcttcct agagctcttc tacatgtatg atgaggacgg ctatcagtcc
     5761 tactgcaccg tgtgctgtga gggccgtgaa ctgctgctgt gcagtaacac aagctgctgc
     5821 agatgcttct gtgtggagtg tctggaggtg ctggtgggcg caggcacagc tgaggatgcc
     5881 aagctgcagg aaccctggag ctgctatatg tgcctccctc agcgctgcca tggggtcctc
     5941 cgacgcagga aagattggaa catgcgcctg caagacttct tcactactga tcctgacctg
     6001 gaagaatttc aggagccacc caagttgtac ccagcaattc ctgcagccaa aaggaggccc
     6061 attagagtcc tgtctctgtt tgatggaatt gcaacggggt acttggtgct caaggagttg
     6121 ggtattaaag tggaaaagta cattgcctcc gaagtctgtg cagagtccat cgctgtggga
     6181 actgttaagc atgaaggcca gatcaaatat gtcaatgacg tccggaaaat caccaagaaa
     6241 aatattgaag agtggggccc gttcgacttg gtgattggtg gaagcgtcga caatgatctc
     6301 tctaacgtca atcctgcccg caaaggttta tatgagggca caggaaggct cttcttcgag
     6361 ttttaccact tgctgaatta tacccgcccc aaggagggcg acaaccgtcc attcttctgg
     6421 atgttcgaga atgttgtggc catgaaagtg aatgacaaga aagacatctc aagattcctg
     6481 gcatgtaacc cagtgatgat cgatgccatc aaggtgtctg ctgctcacag ggcccggtac
     6541 ttctggggta acctacccgg aatgaacagg cccgtgatgg cttcaaagaa tgataagctc
     6601 gagctgcagg actgcctgga gttcagtagg acagcaaagt taaagaaagt gcagacaata
     6661 accaccaagt cgaactccat cagacagggc aaaaaccagc ttttccctgt agtcatgaat
     6721 ggcaaggacg acgttttgtg gtgcactgag ctcgaaagga tcttcggctt ccctgctcac
     6781 tacacggacg tgtccaacat gggccgcggc gcccgtcaga agctgctggg caggtcctgg
     6841 agtgtaccgg tcatcagaca cctgtttgcc cccttgaagg actactttgc ctgtgaatag
     6901 ggtaccaggt aagtgtaccc aattcgccct atagtgagtc gtattacaat tcactcgatc
     6961 gcccttccca acagttgcgc agcctgaatg gcgaatggag atccaatttt taagtgtata
     7021 atgtgttaaa ctactgattc taattgtttg tgtattttag attcacagtc ccaaggctca
     7081 tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata ccacatttgt
     7141 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     7201 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     7261 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     7321 caaactcatc aatgtatctt aaagacacct cgagaccatt accctgttat ccctagaatt
     7381 cgcggccgca atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7441 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7501 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7561 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7621 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7681 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7741 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     7801 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     7861 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
     7921 aacagcatcc ccatctctga agattacagc gtcgccagcg cagctctctc tagcgacggc
     7981 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
     8041 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
     8101 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
     8161 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
     8221 cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag tcgacaatca acctctggat
     8281 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8341 ggatacgctg ctttaatgcc tttgtatcat gcgttaacta aacttgttta ttgcagctta
     8401 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8461 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggaattgact
     8521 caaatgatgt caattagtct atcagaagct catctggtct cccttccggg ggacaagaca
     8581 tccctgttta atatttaaac agcagtgttc ccaaactggg ttcttatatc ccttgctctg
     8641 gtcaaccagg ttgcagggtt tcctgtcctc acaggaacga agtccctaaa gaaacagtgg
     8701 cagccaggtt tagccccgga attgactgga ttcctttttt agggcccatt ggtatggctt
     8761 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     8821 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     8881 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     8941 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct gatatctata
     9001 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     9061 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     9121 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     9181 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     9241 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     9301 taatctagct gcatcaggat catatcgtcg ggtctttttt ccggctcagt catcgcccaa
     9361 gctggcgcta tctgggcatc ggggaggaag aagcccgtgc cttttcccgc gaggttgaag
     9421 cggcatggaa agagtttgcc gaggatgact gctgctgcat tgacgttgag cgaaaacgca
     9481 cgtttaccat gatgattcgg gaaggtgtgg ccatgcacgc ctttaacggt gaactgttcg
     9541 ttcaggccac ctgggatacc agttcgtcgc ggcttttccg gacacagttc cggatggtca
     9601 gcccgaagcg catcagcaac ccgaacaata ccggcgacag ccggaactgc cgtgccggtg
     9661 tgcagattaa tgacagcggt gcggcgctgg gatattacgt cagcgaggac gggtatcctg
     9721 gctggatgcc gcagaaatgg acatggatac cccgtgagtt acccggcggg cgcgcttggc
     9781 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9841 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9901 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9961 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10021 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10081 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10141 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10201 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10261 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10321 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10381 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10441 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10501 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10561 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10621 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    10681 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10741 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
//
