LOCUS       sequence-401137-        5438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for bacterial expression of human protein ARL9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5438)
  TITLE     Kahn Lab Plasmids 2023
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET3-ARL9-His6
FEATURES             Location/Qualifiers
     source          1..5438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     73..92
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        73..91
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             123..145
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             966..983
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1037..1055)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1051..1098
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(1464..1469,1470..1486,1487..1492))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(1519..1537)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1605..1709
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1710..1778,1779..2570)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1928..1947)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2741..3329
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3230..3249
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3483..3500
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3515..3657
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3583..3602)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3743..3765
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3759..3950)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     5408..5427
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg
       61 atcccgcgaa attaatacga ctcactatag ggagaccaca acggtttccc tctagaaata
      121 attttgttta actttaagaa ggagatatac atatggagag ggggaaagtg aagaagaaag
      181 agaaggaaaa ggagacgcag gaggagaaaa tcggagaaaa gggtagggaa gagaaagtga
      241 aaagaaagga ggtggagcag aaaattaaac aaaagcaaga gaagcaggag aggagaaagg
      301 gaaaagagaa agaggaaaag aggacaaagc aagggaagga gacaaacaaa gagaaggaac
      361 aatttaaggg acaagaagag aaaggggaga acaaggacag caccttgaca aggaccccgc
      421 tcgagccgct ggagaaaaac aagcaaatcc tagtgctggg cctggatgga gcaggaaaaa
      481 ccagtgtcct gcactctcta gcttcaaaca gagtccagca cagtgtggca cccacccaag
      541 gtttccatgc agtttgcatc aacactgaag acagccagat ggagttcctg gagattggtg
      601 gcagtaaacc ttttcggtcc tactgggaaa tgtacctatc caagggattg ctgctgatct
      661 ttgtggtgga ttcagcagat cacagccgat tacctgaagc caagaaatac cttcatcagc
      721 taattgcagc aaacccagta cttcctctgg ttgtgtttgc aaacaaacag gatcttgaag
      781 cagcctatca cattacagat atccatgaag ctttggcatt atctgaagtg ggaaatgaca
      841 ggaagatgtt cttgtttgga acctacctga ctaagaatgg ctcagagata ccctccacca
      901 tgcaagatgc caaagacttg attgcacagc tggctgcaga tgtgcagggc agcggcagcg
      961 gcagccatca ccatcaccat cactaaggat ccggctgcta acaaagcccg aaaggaagct
     1021 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     1081 gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt
     1141 ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg
     1201 gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc
     1261 atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg
     1321 caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt
     1381 gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca
     1441 atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga
     1501 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1561 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1621 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1681 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1741 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1801 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1861 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1921 aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc
     1981 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2041 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2101 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2161 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2221 taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac
     2281 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2341 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2401 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2461 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2521 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2581 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2641 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2701 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2761 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2821 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2881 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2941 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3001 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3061 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3121 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3181 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3241 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3301 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3361 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3421 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3481 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3541 atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc
     3601 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     3661 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     3721 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     3781 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag
     3841 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
     3901 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
     3961 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
     4021 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
     4081 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc
     4141 gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg
     4201 aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg
     4261 aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt
     4321 cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg
     4381 gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag
     4441 atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg
     4501 gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat
     4561 ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc
     4621 gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt
     4681 tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag
     4741 ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct
     4801 gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca
     4861 taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt
     4921 cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
     4981 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
     5041 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
     5101 gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc
     5161 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc
     5221 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     5281 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
     5341 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     5401 ccccatcggt gatgtcggcg atataggcgc cagcaacc
//
