LOCUS       sequence-401153-       10380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  jAspSnFR3-mRuby3 in lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10380)
  AUTHORS   Davidsen K, Marvin JS, Aggarwal A, Brown TA, Sullivan LB
  TITLE     An engineered biosensor enables dynamic aspartate measurements in 
            living cells.
  JOURNAL   eLife. 2024 Feb 23;12:RP90024. doi: 10.7554/eLife.90024.
  PUBMED    38393319
REFERENCE   2  (bases 1 to 10380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-02-23"; volume: "12:RP90024. doi"; pages: " 
            10.7554/eLife.90024"
COMMENT     Sequence Label: pLX304-CAG-jAspSnFR3-mRuby3
FEATURES             Location/Qualifiers
     source          1..10380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        286..563
                     /label=chicken beta-actin promoter
     primer_bind     842..864
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     926..945
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      979..988
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1956..1977
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2080..2101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             3319..3336
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    3360..3948
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3413..3433)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3831..3842)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4020..4253
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4331..4452
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4368..4387)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4422..4441
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4492..4627
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4647..4666)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4648..4666)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4676..4693)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4676..4692)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4685..4707)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4834..5289
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4921..4940)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5131..5152
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5315..5419
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5420..5488,5489..6280)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5638..5657)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6451..7039
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6940..6959
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7193..7210
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7327..7348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7363..7368,7369..7386,7387..7393)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7401..7417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7406..7428
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7425..7441
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7425..7441
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7460..7480
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7462..7480
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7508..7734
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7735..7915
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7962..8087
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8580..8813
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8998..9042
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9217..9717
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9636..9654
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9729..10124
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNEKRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10184..10301
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cggggtcatt agttcatagc gctctaggaa gagtaccatt gacgtcaata atgacgtatg
       61 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      121 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      181 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      241 ctacttggca gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca
      301 cgttctgctt cactctcccc atctcccccc cctccccacc cccaattttg tatttattta
      361 ttttttaatt attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccrggsg
      421 gggsggggsg gggsgrgggg sggggsgggg cgaggcggag aggtgcggcg gcagccaatc
      481 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata
      541 aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct
      601 ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga
      661 gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg
      721 tttcttttct gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg
      781 ggagcggctc ggggctgtcc gcggggggac ggctgccttc gggggggacg gggcagggcg
      841 gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca tgttcatgcc
      901 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
      961 ggcaaagaat tggatcccgc caccatgggc agatctgccg caggcagcac gctggacaaa
     1021 atcgccaaaa acggtgtgat tgtcgtcggt caccgtaaat ctgccgtgcc tttctcttat
     1081 tacgacagtc agcacaaagt ggtgggttac tcgcaggaat actccaacgc cattgttgaa
     1141 gcagtgaaaa agaaactcaa caaaccggac ttgcaggtaa aactgattcc gattacccca
     1201 caaaaccgta ttccactgct gcaaaacggc acttacgatt ttgaatgtgg ttctaccacc
     1261 aacaacgtcg aacgccaaaa acaggcggct ttctctgaca ctattttcgt ggtcggtacg
     1321 cgcctgttga ccaaaaaggg tggcgatatc aaagattttg ccaacctgaa agacaaagcc
     1381 gtagtcgtca cttccggcac tacctctgaa gttttgctca acaaactgaa tgaagagcaa
     1441 aaaatgaata tgcgcatcat cagcgccaaa gatcacggtg actctttccg caccctggaa
     1501 agcggtcgtg ccgttgcctt tatgatggat gacgtgctgc tggccggtga acgtgcgaaa
     1561 gcgaagaaac cagacgactg ggaaatcgtc ggcaagccgc agtctcagga ggcctacggt
     1621 tgtatgttgc gtaaagatga tccgcagttc aaaaagctga tggatgacac catcgctcag
     1681 gtgcagacct ccggtgaagc ggaaaaatgg tttgataagt ggttcaaaaa tccaattctg
     1741 gtgagccaca tcgtctatat caccgccgac aagcagaaga acggcatcaa ggcgaacttc
     1801 aagatccacc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1861 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctatcagtcc
     1921 gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1981 gccaccacga actctctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
     2041 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2101 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     2161 ctaaccctga agctcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2221 accaccctgg gctacggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     2281 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cagcttcaag
     2341 gacgacggaa cctacaagac ccgcgccgag gtgaagttag agggcgacac cctggtgaac
     2401 cgcatcgagc taaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2461 gagtacaact ttaacaaccc cctgaacatg aatttcgaac tgtcagacga aatgaaagca
     2521 ctgttcaaag aaccgaatga caaggcactg aaactgcagt ctaccggctc cggaaatgct
     2581 gtgggccagg acacgcagga gcgtatggtg tctaagggcg aagagctgat caaggaaaat
     2641 atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc accaattcaa atgcacaggt
     2701 gaaggagaag gcaggccgta cgagggagtg caaaccatga ggatcaaagt catcgaggga
     2761 ggacccctgc catttgcctt tgacattctt gccacgtcgt tcatgtatgg cagccgtact
     2821 tttatcaagt acccggcaga cattcctgat ttctttaaac agtcctttcc tgagggtttt
     2881 acttgggaaa gagttacgag atacgaagat ggtggagtcg tcaccgtcac gcaggacacc
     2941 agccttgagg atggcgaact cgtttacaac gtcaaagtca gaggggtaaa ctttccctcc
     3001 aatggtcccg tgatgcagaa gaagaccaag ggttgggagc ctaatacaga gatgatgtat
     3061 ccagcagatg gtggtctgag gggatacact gatattgcac tgaaagttga tggtggtggc
     3121 catctgcatt gcaatttcgt aacaacttac aggtcaaaaa agaccgtcgg gaacatcaag
     3181 atgcccggtg tccatgccgt tgatcaccgc ctggaaagga tagaggaaag tgacaatgaa
     3241 acgtacgtag tacaacgcga agtcgcagtt gccaagtact ccaatcttgg tggtggtatg
     3301 gatgaacttt ataagtccca tcaccatcac caccattaag ggtggcgcgt taagtcgaca
     3361 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3421 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3481 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3541 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3601 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3661 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3721 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3781 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3841 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3901 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag
     3961 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     4021 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     4081 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     4141 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     4201 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta
     4261 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     4321 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4381 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4441 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     4501 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4561 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     4621 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     4681 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     4741 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     4801 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     4861 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4921 ctcctttcgc tttcttccct tcctttctcg ccacgttctc cggctttccc cgtcaagctc
     4981 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     5041 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     5101 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     5161 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     5221 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     5281 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5341 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5401 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5461 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5521 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5581 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5641 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5701 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5761 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     5821 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5881 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5941 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6001 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6061 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6121 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6181 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6241 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6301 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6361 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6421 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6481 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6541 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6601 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6661 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6721 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6781 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6841 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6901 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6961 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7021 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7081 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7141 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7201 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     7261 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     7321 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     7381 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     7441 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     7501 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     7561 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     7621 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     7681 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     7741 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7801 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7861 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     7921 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     7981 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     8041 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     8101 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     8161 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     8221 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     8281 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     8341 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     8401 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     8461 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     8521 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     8581 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     8641 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     8701 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     8761 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     8821 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     8881 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     8941 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     9001 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     9061 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     9121 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     9181 tcgtttcaga cccacctccc aaccccgagg ggacccttgc gccttttcca aggcagccct
     9241 gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac
     9301 cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc
     9361 cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg
     9421 ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac
     9481 ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc
     9541 tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt
     9601 ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg
     9661 gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg
     9721 gtaccaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     9781 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     9841 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     9901 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     9961 cgatcggaaa tgagaagagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
    10021 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
    10081 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggcctgcag ctgcagtagt
    10141 aagaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
    10201 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    10261 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    10321 gcagagatcc actttggcgc cggctcgagg gggcccgggg ttattaatag taatcaatta
//
