LOCUS       sequence-401154-       10334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  jAspSnFR3 in lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Davidsen K, Marvin JS, Aggarwal A, Brown TA, Sullivan LB
  TITLE     An engineered biosensor enables dynamic aspartate measurements in 
            living cells.
  JOURNAL   eLife. 2024 Feb 23;12:RP90024. doi: 10.7554/eLife.90024.
  PUBMED    38393319
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-02-23"; volume: "12:RP90024. doi"; pages: " 
            10.7554/eLife.90024"
COMMENT     Sequence Label: pLenti-jAspSnFR3
FEATURES             Location/Qualifiers
     source          1..10334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2220..2446
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2447..2627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2674..2799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3292..3525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3710..3754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4021..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4160..4463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4464..4667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4618..4638
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4664..4688
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4772..4796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4855..4864
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5829..5850
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5953..5974)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(6457..6481)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6512..7100
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6565..6585)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6983..6994)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7156..7655
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7191..7214)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7588..7607
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7673..8698
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7673..8698
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             9072..9305
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9377..9498
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9414..9433)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9468..9487
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9538..9673
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9693..9712)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9694..9712)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9722..9739)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9722..9738)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9731..9753)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9880..10308
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9967..9986)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10177..10198
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3601 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3661 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3721 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3781 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3841 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3901 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3961 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     4021 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4081 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc
     4141 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4201 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4261 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4321 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4381 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4441 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4501 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4561 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4621 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     4681 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct
     4741 agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctttaa
     4801 aggaaccaat tcagtcgact ggatccggta ccgaattcgc ttactaaaag ccaggccacc
     4861 atgggatccg ccgcaggcag cacgctggac aaaatcgcca aaaacggtgt gattgtcgtc
     4921 ggtcaccgta aatctgccgt gcctttctct tattacgaca gtcagcacaa agtggtgggt
     4981 tactcgcagg aatactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg
     5041 gacttgcagg taaaactgat tccgattacc ccacaaaacc gtattccact gctgcaaaac
     5101 ggcacttacg attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg
     5161 gctttctctg acactatttt cgtggtcggt acgcgcctgt tgaccaaaaa gggtggcgat
     5221 atcaaagatt ttgccaacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct
     5281 gaagttttgc tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc
     5341 aaagatcacg gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg
     5401 gatgacgtgc tgctggccgg tgaacgtgcg aaagcgaaga aaccagacga ctgggaaatc
     5461 gtcggcaagc cgcagtctca ggaggcctac ggttgtatgt tgcgtaaaga tgatccgcag
     5521 ttcaaaaagc tgatggatga caccatcgct caggtgcaga cctccggtga agcggaaaaa
     5581 tggtttgata agtggttcaa aaatccaatt ctggtgagcc acatcgtcta tatcaccgcc
     5641 gacaagcaga agaacggcat caaggcgaac ttcaagatcc accacaacgt ggaggacggc
     5701 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5761 ctgcccgaca accactacct gagctatcag tccgtgctga gcaaagaccc caacgagaag
     5821 cgcgatcaca tggtcctgct ggagttcgtg accgccacca cgaactctct cggcatggac
     5881 gagctgtaca agggcggtac cggagggagc atgagcaagg gcgaggagct gttcaccggg
     5941 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgcgc
     6001 ggcgagggcg agggcgatgc caccaacggc aagctaaccc tgaagctcat ctgcaccacc
     6061 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cgtgcagtgc
     6121 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     6181 ggctacgtcc aggagcgcac catcagcttc aaggacgacg gaacctacaa gacccgcgcc
     6241 gaggtgaagt tagagggcga caccctggtg aaccgcatcg agctaaaggg catcgacttc
     6301 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actttaacaa ccccctgaac
     6361 atgaatttcg aactgtcaga cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca
     6421 ctgaaataag aattcgaagc ttgatccggc tgctagaccc agctttcttg tacaaagtgg
     6481 ttgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca aaatttgtga
     6541 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6601 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6661 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6721 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6781 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6841 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6901 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6961 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     7021 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     7081 cctttgggcc gcctccccgc ctggaattct gcagatatcc ggactagtga tctctcgagg
     7141 ttaacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     7201 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     7261 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     7321 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     7381 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     7441 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     7501 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     7561 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     7621 cgctgttctc ctcttcctca tctccgggcc tttcgacctg catcccgcca ccatgaaaaa
     7681 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     7741 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     7801 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     7861 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     7921 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     7981 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     8041 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     8101 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     8161 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     8221 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     8281 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     8341 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     8401 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     8461 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     8521 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     8581 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     8641 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag
     8701 tagatgccga ccgaacaaga gctgatttcg agaacgcctc agccagcaac tcgcgcgagc
     8761 ctagcaaggc aaatgcgaga gaacggcctt acgcttggtg gcacagttct cgtccacagt
     8821 tcgctaagct cgctcggctg ggtcgcggga gggccggtcg cagtgattca ggcccttctg
     8881 gattgtgttg gtccccaggg cacgattgtc atgcccacgc actcgggtga tctgactgat
     8941 cccgcagatt ggagatcgcc gcccgtgcct gccgattggg tgcagatccg tcgagggccc
     9001 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     9061 aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg
     9121 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     9181 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     9241 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     9301 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     9361 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9421 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     9481 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     9541 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9601 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9661 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     9721 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     9781 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     9841 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     9901 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     9961 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    10021 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    10081 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    10141 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    10201 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    10261 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
    10321 taacgcttac aatt
//
