LOCUS       sequence-401155-        7868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LOXL2 mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Wang H, Poe A, Martinez Yus M, Pak L, Nandakumar K, Santhanam L
  TITLE     Lysyl oxidase-like 2 processing by factor Xa modulates its activity 
            and substrate preference.
  JOURNAL   Commun Biol. 2023 Apr 7;6(1):375. doi: 10.1038/s42003-023-04748-8.
  PUBMED    37029269
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-04-7"; volume: "6(1):375. doi"; pages: " 
            10.1038/s42003-023-04748-8"
COMMENT     Sequence Label: hLOXL2-S300P
FEATURES             Location/Qualifiers
     source          1..7868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(629..1420,1421..1489))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1252..1271
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1490..1594)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1669..1688)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1860..2239
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2240..2443
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2394..2414
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2488..2507
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2488..2506
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2575..2599
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4931..4955)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4978..5007
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5023..5040
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5084..5101)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5090..5314
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5360..5788
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5447..5466)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5657..5678
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5797..5817)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5802..6131
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5982..6117
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6044..6063
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6198..6992
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6252..6271)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6862..6881
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7166..7287
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7203..7222)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7257..7276
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7336..7352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7336..7352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7349..7371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7360..7376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7384..7390,7391..7408,7409..7414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7429..7450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7567..7584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(7738..7868,1..458))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7818..7837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
       61 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
      121 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
      181 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
      241 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
      301 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
      361 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
      421 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
      481 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
      541 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      601 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
      661 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
      721 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
      781 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
      841 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
      901 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
      961 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     1021 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     1081 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     1141 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     1201 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     1261 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     1321 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     1381 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     1441 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     1501 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     1561 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     1621 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
     1681 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
     1741 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     1801 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
     1861 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     1921 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     1981 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2041 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2101 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2161 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2221 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2281 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2341 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2401 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
     2461 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
     2521 ctagcgttta aacgggccct ctagactcga gcggccgcca tgcaattgat taatacaagt
     2581 ttgtacaaaa aagcaggctc caccatggag aggcctctgt gctcccacct ctgcagctgc
     2641 ctggctatgc tggccctcct gtcccccctg agcctggcac agtatgacag ctggccccat
     2701 taccccgagt acttccagca accggctcct gagtatcacc agccccaggc ccccgccaac
     2761 gtggccaaga ttcagctgcg cctggctggg cagaagagga agcacagcga gggccgggtg
     2821 gaggtgtact atgatggcca gtggggcacc gtgtgcgatg acgacttctc catccacgct
     2881 gcccacgtcg tctgccggga gctgggctac gtggaggcca agtcctggac tgccagctcc
     2941 tcctacggca agggagaagg gcccatctgg ttagacaatc tccactgtac tggcaacgag
     3001 gcgacccttg cagcatgcac ctccaatggc tggggcgtca ctgactgcaa gcacacggag
     3061 gatgtcggtg tggtgtgcag cgacaaaagg attcctgggt tcaaatttga caattcgttg
     3121 atcaaccaga tagagaacct gaatatccag gtggaggaca ttcggattcg agccatcctc
     3181 tcaacctacc gcaagcgcac cccagtgatg gagggctacg tggaggtgaa ggagggcaag
     3241 acctggaagc agatctgtga caagcactgg acggccaaga attcccgcgt ggtctgcggc
     3301 atgtttggct tccctgggga gaggacatac aataccaaag tgtacaaaat gtttgcctca
     3361 cggaggaagc agcgctactg gccattctcc atggactgca ccggcacaga ggcccacatc
     3421 tccagctgca agctgggccc ccaggtgtca ctggacccca tgaagaatgt cacctgcgag
     3481 aatgggctac cggccgtggt gccttgtgtg cctgggcagg tcttcagccc tgacggaccc
     3541 tcgagattcc ggaaagcgta caagccagag caacccctgg tgcgactgag aggcggtgcc
     3601 tacatcgggg agggccgcgt ggaggtgctc aaaaatggag agtgggggac cgtctgcgac
     3661 gacaagtggg acctggtgtc ggccagtgtg gtctgcagag agctgggctt tgggagtgcc
     3721 aaagaggcag tcactggctc ccgactgggg caagggatcg gacccatcca cctcaacgag
     3781 atccagtgca caggcaatga gaagtccatt atagactgca agttcaatgc cgagtctcag
     3841 ggctgcaacc acgaggagga tgctggtgtg agatgcaaca cccctgccat gggcttgcag
     3901 aagaagctgc gcctgaacgg cggccgcaat ccctacgagg gccgagtgga ggtgctggtg
     3961 gagagaaacg ggtcccttgt gtgggggatg gtgtgtggcc aaaactgggg catcgtggag
     4021 gccatggtgg tctgccgcca gctgggcctg ggattcgcca gcaacgcctt ccaggagacc
     4081 tggtattggc acggagatgt caacagcaac aaagtggtca tgagtggagt gaagtgctcg
     4141 ggaacggagc tgtccctggc gcactgccgc cacgacgggg aggacgtggc ctgcccccag
     4201 ggcggagtgc agtacggggc cggagttgcc tgctcagaaa ccgcccctga cctggtcctc
     4261 aatgcggaga tggtgcagca gaccacctac ctggaggacc ggcccatgtt cctgctgcag
     4321 tgtgccatgg aggagaactg cctctcggcc tcagccgcgc agaccgaccc caccacgggc
     4381 taccgccggc tcctgcgctt ctcctcccag atccacaaca atggccagtc cgacttccgg
     4441 cccaagaacg gccgccacgc gtggatctgg cacgactgtc acaggcacta ccacagcatg
     4501 gaggtgttca cccactatga cctgctgaac ctcaatggca ccaaggtggc agagggccac
     4561 aaggccagct tctgcttgga ggacacagaa tgtgaaggag acatccagaa gaattacgag
     4621 tgtgccaact tcggcgatca gggcatcacc atgggctgct gggacatgta ccgccatgac
     4681 atcgactgcc agtgggttga catcactgac gtgccccctg gagactacct gttccaggtt
     4741 gttattaacc ccaacttcga ggttgcagaa tccgattact ccaacaacat catgaaatgc
     4801 aggagccgct atgacggcca ccgcatctgg atgtacaact gccacatagg tggttccttc
     4861 agcgaagaga cggaaaaaaa gtttgagcac ttcagcgggc tcttaaacaa ccagctgtcc
     4921 ccgcagttgg acccagcttt cttgtacaaa gtggtcctcg aggtaccaag ctttctagaa
     4981 caaaaactca tctcagaaga ggatctgaat agcgccgtcg accatcatca tcatcatcat
     5041 tgagtttaaa cggtctccag cttaagttta aaccgctgat cagcctcgac tgtgccttct
     5101 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     5161 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     5221 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     5281 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     5341 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     5401 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     5461 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     5521 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     5581 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5641 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5701 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5761 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     5821 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5881 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5941 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     6001 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6061 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6121 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag
     6181 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     6241 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6301 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6361 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     6421 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     6481 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     6541 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     6601 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     6661 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     6721 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     6781 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     6841 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     6901 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     6961 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     7021 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     7081 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     7141 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
     7201 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7261 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     7321 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7381 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7441 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     7501 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     7561 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7621 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7681 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7741 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7801 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7861 tcctgttc
//
