LOCUS       sequence-401156-       10688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mitochondrial directed jAspSnFR3 in lentiviral expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10688)
  AUTHORS   Davidsen K, Marvin JS, Aggarwal A, Brown TA, Sullivan LB
  TITLE     An engineered biosensor enables dynamic aspartate measurements in 
            living cells.
  JOURNAL   eLife. 2024 Feb 23;12:RP90024. doi: 10.7554/eLife.90024.
  PUBMED    38393319
REFERENCE   2  (bases 1 to 10688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2024-02-23"; volume: "12:RP90024. doi"; pages: " 
            10.7554/eLife.90024"
COMMENT     Sequence Label: pLenti-MTS-jAspSnFR3
FEATURES             Location/Qualifiers
     source          1..10688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2172..2192
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2220..2446
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2447..2627
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2674..2799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3292..3525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3710..3754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4021..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        4160..4463
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4464..4667
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4618..4638
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4664..4688
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    4772..4796
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4855..4864
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     6183..6204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6307..6328)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     protein_bind    complement(6811..6835)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6866..7454
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6919..6939)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7337..7348)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7510..8009
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7545..7568)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7942..7961
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8027..9052
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8027..9052
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             9426..9659
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9731..9852
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9768..9787)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9822..9841
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9892..10027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10047..10066)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10048..10066)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10076..10093)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10076..10092)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10085..10107)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10234..10662
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10321..10340)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10531..10552
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     2461 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     2521 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     2581 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     2641 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     2701 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     2761 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     2821 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     2881 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     2941 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     3001 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     3061 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     3121 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     3181 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     3241 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     3301 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     3361 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     3421 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     3481 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     3541 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     3601 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     3661 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     3721 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     3781 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     3841 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     3901 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     3961 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcggttaac
     4021 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     4081 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc
     4141 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     4201 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4261 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4321 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4381 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4441 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     4501 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4561 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     4621 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     4681 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct
     4741 agtccagtgt ggtggaattc tgcagatatc aacaagtttg tacaaaaaag caggctttaa
     4801 aggaaccaat tcagtcgact ggatccggta ccgaattcgc ttactaaaag ccaggccacc
     4861 atggcactgc ttcgcgccgc cgtttcagaa ctcagacgga gaggacgggg tgcgcttact
     4921 cccctcccgg cgctgtctag cttgctttcc tcacttagcc cccgaagtcc cgcctcaacg
     4981 cgcccagagc caaacaatcc acacgcagat cgacgccatg tcatcgcttt gaggcgatgc
     5041 cccccacttc ctgcctctgc cgttctggca cctgaactcc tgcatgcacg aggattgctc
     5101 ccgagacatt ggtctcatgc ctctcccttg tccacgtcct cttcatccag tagaccagca
     5161 gataaggcgc agttgacctg ggtcgataaa tggatcccag aagccgcgag accgtatgga
     5221 tccgccgcag gcagcacgct ggacaaaatc gccaaaaacg gtgtgattgt cgtcggtcac
     5281 cgtaaatctg ccgtgccttt ctcttattac gacagtcagc acaaagtggt gggttactcg
     5341 caggaatact ccaacgccat tgttgaagca gtgaaaaaga aactcaacaa accggacttg
     5401 caggtaaaac tgattccgat taccccacaa aaccgtattc cactgctgca aaacggcact
     5461 tacgattttg aatgtggttc taccaccaac aacgtcgaac gccaaaaaca ggcggctttc
     5521 tctgacacta ttttcgtggt cggtacgcgc ctgttgacca aaaagggtgg cgatatcaaa
     5581 gattttgcca acctgaaaga caaagccgta gtcgtcactt ccggcactac ctctgaagtt
     5641 ttgctcaaca aactgaatga agagcaaaaa atgaatatgc gcatcatcag cgccaaagat
     5701 cacggtgact ctttccgcac cctggaaagc ggtcgtgccg ttgcctttat gatggatgac
     5761 gtgctgctgg ccggtgaacg tgcgaaagcg aagaaaccag acgactggga aatcgtcggc
     5821 aagccgcagt ctcaggaggc ctacggttgt atgttgcgta aagatgatcc gcagttcaaa
     5881 aagctgatgg atgacaccat cgctcaggtg cagacctccg gtgaagcgga aaaatggttt
     5941 gataagtggt tcaaaaatcc aattctggtg agccacatcg tctatatcac cgccgacaag
     6001 cagaagaacg gcatcaaggc gaacttcaag atccaccaca acgtggagga cggcagcgtg
     6061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     6121 gacaaccact acctgagcta tcagtccgtg ctgagcaaag accccaacga gaagcgcgat
     6181 cacatggtcc tgctggagtt cgtgaccgcc accacgaact ctctcggcat ggacgagctg
     6241 tacaagggcg gtaccggagg gagcatgagc aagggcgagg agctgttcac cggggtggtg
     6301 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gcgcggcgag
     6361 ggcgagggcg atgccaccaa cggcaagcta accctgaagc tcatctgcac caccggcaag
     6421 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcgtgca gtgcttcgcc
     6481 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     6541 gtccaggagc gcaccatcag cttcaaggac gacggaacct acaagacccg cgccgaggtg
     6601 aagttagagg gcgacaccct ggtgaaccgc atcgagctaa agggcatcga cttcaaggag
     6661 gacggcaaca tcctggggca caagctggag tacaacttta acaaccccct gaacatgaat
     6721 ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa ggcactgaaa
     6781 taagaattcg aagcttgatc cggctgctag acccagcttt cttgtacaaa gtggttgata
     6841 tccagcacag tggcggccgc tcgacaatca acctctggat tacaaaattt gtgaaagatt
     6901 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6961 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     7021 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     7081 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     7141 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     7201 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     7261 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     7321 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     7381 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     7441 ggccgcctcc ccgcctggaa ttctgcagat atccggacta gtgatctctc gaggttaacg
     7501 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     7561 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     7621 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     7681 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     7741 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     7801 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     7861 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     7921 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     7981 tctcctcttc ctcatctccg ggcctttcga cctgcatccc gccaccatga aaaagcctga
     8041 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     8101 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     8161 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     8221 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     8281 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     8341 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     8401 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     8461 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     8521 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     8581 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     8641 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     8701 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     8761 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     8821 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     8881 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     8941 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     9001 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat agagtagatg
     9061 ccgaccgaac aagagctgat ttcgagaacg cctcagccag caactcgcgc gagcctagca
     9121 aggcaaatgc gagagaacgg ccttacgctt ggtggcacag ttctcgtcca cagttcgcta
     9181 agctcgctcg gctgggtcgc gggagggccg gtcgcagtga ttcaggccct tctggattgt
     9241 gttggtcccc agggcacgat tgtcatgccc acgcactcgg gtgatctgac tgatcccgca
     9301 gattggagat cgccgcccgt gcctgccgat tgggtgcaga tccgtcgagg gcccggtacc
     9361 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     9421 gggactggaa gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg
     9481 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     9541 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     9601 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     9661 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     9721 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9781 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9841 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     9901 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     9961 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    10021 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
    10081 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
    10141 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
    10201 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
    10261 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
    10321 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
    10381 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
    10441 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
    10501 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
    10561 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
    10621 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
    10681 ttacaatt
//
