LOCUS       sequence-401159-        6854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LOXL2 deletion mutant lacking 1-338 amino acids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Wang H, Poe A, Martinez Yus M, Pak L, Nandakumar K, Santhanam L
  TITLE     Lysyl oxidase-like 2 processing by factor Xa modulates its activity 
            and substrate preference.
  JOURNAL   Commun Biol. 2023 Apr 7;6(1):375. doi: 10.1038/s42003-023-04748-8.
  PUBMED    37029269
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-04-7"; volume: "6(1):375. doi"; pages: " 
            10.1038/s42003-023-04748-8"
COMMENT     Sequence Label: DN-LOXL2
FEATURES             Location/Qualifiers
     source          1..6854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(80..96)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(80..96)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(93..115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    104..120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(128..134,135..152,153..158))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    173..194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(311..328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(482..1070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(562..581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1241..2032,2033..2101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1864..1883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2102..2206)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2281..2300)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2472..2851
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2852..3055
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3006..3026
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3100..3119
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3100..3118
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3187..3211
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4529..4553)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4576..4605
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4621..4638
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4682..4699)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4688..4912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4958..5386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5045..5064)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5255..5276
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5395..5415)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5400..5729
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5580..5715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5642..5661
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5796..6590
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5850..5869)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6460..6479
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    join(6764..6854,1..31)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6801..6820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
       61 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     1201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     2161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2221 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     2281 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2341 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2401 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2461 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2521 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2581 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2641 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2701 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2761 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2821 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2881 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2941 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     3001 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
     3061 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
     3121 gacccaagct ggctagcgtt taaacgggcc ctctagactc gagcggccgc catgcaattg
     3181 attaatacaa gtttgtacaa aaaagcaggc ttcaccatgg tggaggtgct caaaaatgga
     3241 gagtggggga ccgtctgcga cgacaagtgg gacctggtgt cggccagtgt ggtctgcaga
     3301 gagctgggct ttgggagtgc caaagaggca gtcactggct cccgactggg gcaagggatc
     3361 ggacccatcc acctcaacga gatccagtgc acaggcaatg agaagtccat tatagactgc
     3421 aagttcaatg ccgagtctca gggctgcaac cacgaggagg atgctggtgt gagatgcaac
     3481 acccctgcca tgggcttgca gaagaagctg cgcctgaacg gcggccgcaa tccctacgag
     3541 ggccgagtgg aggtgctggt ggagagaaac gggtcccttg tgtgggggat ggtgtgtggc
     3601 caaaactggg gcatcgtgga ggccatggtg gtctgccgcc agctgggcct gggattcgcc
     3661 agcaacgcct tccaggagac ctggtattgg cacggagatg tcaacagcaa caaagtggtc
     3721 atgagtggag tgaagtgctc gggaacggag ctgtccctgg cgcactgccg ccacgacggg
     3781 gaggacgtgg cctgccccca gggcggagtg cagtacgggg ccggagttgc ctgctcagaa
     3841 accgcccctg acctggtcct caatgcggag atggtgcagc agaccaccta cctggaggac
     3901 cggcccatgt tcctgctgca gtgtgccatg gaggagaact gcctctcggc ctcagccgcg
     3961 cagaccgacc ccaccacggg ctaccgccgg ctcctgcgct tctcctccca gatccacaac
     4021 aatggccagt ccgacttccg gcccaagaac ggccgccacg cgtggatctg gcacgactgt
     4081 cacaggcact accacagcat ggaggtgttc acccactatg acctgctgaa cctcaatggc
     4141 accaaggtgg cagagggcca caaggccagc ttctgcttgg aggacacaga atgtgaagga
     4201 gacatccaga agaattacga gtgtgccaac ttcggcgatc agggcatcac catgggctgc
     4261 tgggacatgt accgccatga catcgactgc cagtgggttg acatcactga cgtgccccct
     4321 ggagactacc tgttccaggt tgttattaac cccaacttcg aggttgcaga atccgattac
     4381 tccaacaaca tcatgaaatg caggagccgc tatgacagcc accgcatctg gatgtacaac
     4441 tgccacatag gtggttcctt cagcgaagag acggaaaaaa agtttgagca cttcagcggg
     4501 ctcttaaaca accagctgtc cccgcagaac ccagctttct tgtacaaagt ggtcctcgag
     4561 gtaccaagct ttctagaaca aaaactcatc tcagaagagg atctgaatag cgccgtcgac
     4621 catcatcatc atcatcattg agtttaaacg gtctccagct taagtttaaa ccgctgatca
     4681 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4741 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4801 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4861 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     4921 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     4981 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5041 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5101 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5161 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5221 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5281 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5341 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     5401 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5461 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5521 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5581 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5641 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5701 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5761 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5821 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5881 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5941 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     6001 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     6061 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     6121 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     6181 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     6241 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     6301 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     6361 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     6421 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     6481 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     6541 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     6601 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6661 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6721 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6781 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6841 actgcattct agtt
//
