LOCUS       sequence-401160-        7868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Point mutant of hLOXL2 resistant to processing by Factor Xa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Wang H, Poe A, Martinez Yus M, Pak L, Nandakumar K, Santhanam L
  TITLE     Lysyl oxidase-like 2 processing by factor Xa modulates its activity 
            and substrate preference.
  JOURNAL   Commun Biol. 2023 Apr 7;6(1):375. doi: 10.1038/s42003-023-04748-8.
  PUBMED    37029269
REFERENCE   2  (bases 1 to 7868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-04-7"; volume: "6(1):375. doi"; pages: " 
            10.1038/s42003-023-04748-8"
COMMENT     Sequence Label: R338G/V339P-LOXL2
FEATURES             Location/Qualifiers
     source          1..7868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..24
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(141..158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(312..900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(392..411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1071..1862,1863..1931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1694..1713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1932..2036)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2111..2130)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2302..2681
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2682..2885
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2836..2856
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2930..2949
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2930..2948
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3017..3041
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5373..5397)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5420..5449
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5465..5482
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5526..5543)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5532..5756
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5802..6230
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5889..5908)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6099..6120
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6239..6259)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6244..6573
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6424..6559
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6486..6505
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6640..7434
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6694..6713)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7304..7323
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7608..7729
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7645..7664)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7699..7718
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7778..7794)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7778..7794)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7791..7813)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7802..7818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7826..7832,7833..7850,7851..7856))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
       61 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      121 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      181 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
      241 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      301 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      361 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      421 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      481 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      541 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      601 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      661 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      721 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      781 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      841 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      901 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      961 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     1021 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     1081 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     1141 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     1201 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     1261 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     1321 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1381 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1441 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1501 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1561 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1621 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1681 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1741 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1801 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1861 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1921 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1981 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2041 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     2101 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     2161 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     2221 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     2281 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     2341 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     2401 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     2461 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     2521 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     2581 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     2641 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     2701 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     2761 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     2821 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     2881 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     2941 actataggga gacccaagct ggctagcgtt taaacgggcc ctctagactc gagcggccgc
     3001 catgcaattg attaatacaa gtttgtacaa aaaagcaggc tccaccatgg agaggcctct
     3061 gtgctcccac ctctgcagct gcctggctat gctggccctc ctgtcccccc tgagcctggc
     3121 acagtatgac agctggcccc attaccccga gtacttccag caaccggctc ctgagtatca
     3181 ccagccccag gcccccgcca acgtggccaa gattcagctg cgcctggctg ggcagaagag
     3241 gaagcacagc gagggccggg tggaggtgta ctatgatggc cagtggggca ccgtgtgcga
     3301 tgacgacttc tccatccacg ctgcccacgt cgtctgccgg gagctgggct acgtggaggc
     3361 caagtcctgg actgccagct cctcctacgg caagggagaa gggcccatct ggttagacaa
     3421 tctccactgt actggcaacg aggcgaccct tgcagcatgc acctccaatg gctggggcgt
     3481 cactgactgc aagcacacgg aggatgtcgg tgtggtgtgc agcgacaaaa ggattcctgg
     3541 gttcaaattt gacaattcgt tgatcaacca gatagagaac ctgaatatcc aggtggagga
     3601 cattcggatt cgagccatcc tctcaaccta ccgcaagcgc accccagtga tggagggcta
     3661 cgtggaggtg aaggagggca agacctggaa gcagatctgt gacaagcact ggacggccaa
     3721 gaattcccgc gtggtctgcg gcatgtttgg cttccctggg gagaggacat acaataccaa
     3781 agtgtacaaa atgtttgcct cacggaggaa gcagcgctac tggccattct ccatggactg
     3841 caccggcaca gaggcccaca tctccagctg caagctgggc ccccaggtgt cactggaccc
     3901 catgaagaat gtcacctgcg agaatgggct accggccgtg gtgagttgtg tgcctgggca
     3961 ggtcttcagc cctgacggac cctcgagatt ccggaaagcg tacaagccag agcaacccct
     4021 ggtgcgactg agaggcggtg cctacatcgg ggagggcggc ccggaggtgc tcaaaaatgg
     4081 agagtggggg accgtctgcg acgacaagtg ggacctggtg tcggccagtg tggtctgcag
     4141 agagctgggc tttgggagtg ccaaagaggc agtcactggc tcccgactgg ggcaagggat
     4201 cggacccatc cacctcaacg agatccagtg cacaggcaat gagaagtcca ttatagactg
     4261 caagttcaat gccgagtctc agggctgcaa ccacgaggag gatgctggtg tgagatgcaa
     4321 cacccctgcc atgggcttgc agaagaagct gcgcctgaac ggcggccgca atccctacga
     4381 gggccgagtg gaggtgctgg tggagagaaa cgggtccctt gtgtggggga tggtgtgtgg
     4441 ccaaaactgg ggcatcgtgg aggccatggt ggtctgccgc cagctgggcc tgggattcgc
     4501 cagcaacgcc ttccaggaga cctggtattg gcacggagat gtcaacagca acaaagtggt
     4561 catgagtgga gtgaagtgct cgggaacgga gctgtccctg gcgcactgcc gccacgacgg
     4621 ggaggacgtg gcctgccccc agggcggagt gcagtacggg gccggagttg cctgctcaga
     4681 aaccgcccct gacctggtcc tcaatgcgga gatggtgcag cagaccacct acctggagga
     4741 ccggcccatg ttcctgctgc agtgtgccat ggaggagaac tgcctctcgg cctcagccgc
     4801 gcagaccgac cccaccacgg gctaccgccg gctcctgcgc ttctcctccc agatccacaa
     4861 caatggccag tccgacttcc ggcccaagaa cggccgccac gcgtggatct ggcacgactg
     4921 tcacaggcac taccacagca tggaggtgtt cacccactat gacctgctga acctcaatgg
     4981 caccaaggtg gcagagggcc acaaggccag cttctgcttg gaggacacag aatgtgaagg
     5041 agacatccag aagaattacg agtgtgccaa cttcggcgat cagggcatca ccatgggctg
     5101 ctgggacatg taccgccatg acatcgactg ccagtgggtt gacatcactg acgtgccccc
     5161 tggagactac ctgttccagg ttgttattaa ccccaacttc gaggttgcag aatccgatta
     5221 ctccaacaac atcatgaaat gcaggagccg ctatgacggc caccgcatct ggatgtacaa
     5281 ctgccacata ggtggttcct tcagcgaaga gacggaaaaa aagtttgagc acttcagcgg
     5341 gctcttaaac aaccagctgt ccccgcagtt ggacccagct ttcttgtaca aagtggtcct
     5401 cgaggtacca agctttctag aacaaaaact catctcagaa gaggatctga atagcgccgt
     5461 cgaccatcat catcatcatc attgagttta aacggtctcc agcttaagtt taaaccgctg
     5521 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5581 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5641 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5701 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5761 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5821 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5881 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5941 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6001 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6061 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6121 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6181 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     6241 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6301 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6361 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6421 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6481 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     6541 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     6601 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     6661 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     6721 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     6781 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     6841 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6901 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6961 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     7021 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     7081 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     7141 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     7201 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     7261 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     7321 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     7381 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     7441 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     7501 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7561 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7621 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7681 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7741 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7801 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7861 ggggtgcc
//
