LOCUS       sequence-401163-        9632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression construct encoding the BRAF regulatory (REG) 
            domain (AA 1-435) with a C-terminal HaloTag. The REG domain contains
            the RASopathy mutation, T241P, within its cysteine-rich domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9632)
  AUTHORS   Spencer-Smith R, Terrell EM, Insinna C, Agamasu C, Wagner ME, Ritt 
            DA, Stauffer J, Stephen AG, Morrison DK
  TITLE     RASopathy mutations provide functional insight into the BRAF 
            cysteine-rich domain and reveal the importance of autoinhibition in 
            BRAF regulation.
  JOURNAL   Mol Cell. 2022 Nov 17;82(22):4262-4276.e5. doi: 
            10.1016/j.molcel.2022.10.016. Epub 2022 Nov 7.
  PUBMED    36347258
REFERENCE   2  (bases 1 to 9632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.10.016"; journalName: "Mol Cell"; date: 
            "2022-11-17- 17"; volume: "82"; issue: "22"; pages: "4262-4276.e5"
COMMENT     Sequence Label: pCMV-BRAF-REG-T241P-Halo
FEATURES             Location/Qualifiers
     source          1..9632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      210..1999
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(2036..2055)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2203..2223
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2234..2537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2538..2741
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2692..2712
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2738..2762
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2807..2831
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2832..2841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4137..4161)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4166..5053
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             5066..5086
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     protein_bind    complement(5109..5129)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        complement(5191..5209)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5192..5209)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5229..5246)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5235..5459
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5505..5933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5592..5611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5802..5823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5942..5962)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5947..6276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6127..6262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6189..6208
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6343..7137
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6397..6416)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7007..7026
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7311..7432
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7348..7367)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7402..7421
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7481..7497)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7481..7497)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7494..7516)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7505..7521
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7529..7535,7536..7553,7554..7559))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7574..7595
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7712..7729)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7883..8471)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7963..7982)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8642..9433,9434..9502))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9265..9284
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9503..9607)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgacg gatcgggaga tccgccttta tgtgtaactc ttggctgaag ctcttacacc
       61 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
      121 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
      181 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
      241 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
      301 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
      361 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
      421 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
      481 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
      541 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
      601 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
      661 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
      721 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
      781 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
      841 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
      901 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
      961 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     1021 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     1081 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     1141 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     1201 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     1261 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     1321 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     1381 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     1441 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     1501 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     1561 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     1621 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     1681 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     1741 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     1801 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     1861 ctgtatccgg gtagcatatg ctatcctcat gcatatacag tcagcatatg atacccagta
     1921 gtagagtggg agtgctatcc tttgcatatg ccgccacctc ccaagggggc gtgaattttc
     1981 gctgcttgtc cttttcctgc tggggatctc ccgatcccct atggtgcact ctcagtacaa
     2041 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     2101 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     2161 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg atcaactttg tatagaaaag
     2221 ttggccctgc aggcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     2281 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     2341 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     2401 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     2461 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
     2521 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
     2581 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     2641 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
     2701 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
     2761 tcgcctggag acgccatcca cgctgttttg acctccatag aagacaacaa ctttgtacaa
     2821 aaaagttggc accatggcgg cgctgagcgg tggcggtggt ggcggcgcgg agccgggcca
     2881 ggctctgttc aacggggaca tggagcccga ggccggcgcc ggcgccggcg ccgcggcctc
     2941 ttcggctgcg gaccctgcca ttccggagga ggtgtggaat atcaaacaaa tgattaagtt
     3001 gacacaggaa catatagagg ccctattgga caaatttggt ggggagcata atccaccatc
     3061 aatatatctg gaggcctatg aagaatacac cagcaagcta gatgcactcc aacaaagaga
     3121 acaacagtta ttggaatctc tggggaacgg aactgatttt tctgtttcta gctctgcatc
     3181 aatggatacc gttacatctt cttcctcttc tagcctttca gtgctacctt catctctttc
     3241 agtttttcaa aatcccacag atgtggcacg gagcaacccc aagtcaccac aaaaacctat
     3301 cgttagagtc ttcctgccca acaaacagag gacagtggta cctgcaaggt gtggagttac
     3361 agtccgagac agtctaaaga aagcactgat gatgagaggt ctaatcccag agtgctgtgc
     3421 tgtttacaga atccaggatg gagagaagaa accaattggt tgggacactg atatttcctg
     3481 gcttactgga gaagaattgc atgtggaagt gttggagaat gttccactta caacacacaa
     3541 ctttgtacga aaaccgtttt tcaccttagc attttgtgac ttttgtcgaa agctgctttt
     3601 ccagggtttc cgctgtcaaa catgtggtta taaatttcac cagcgttgta gtacagaagt
     3661 tccactgatg tgtgttaatt atgaccaact tgatttgctg tttgtctcca agttctttga
     3721 acaccaccca ataccacagg aagaggcgtc cttagcagag actgccctaa catctggatc
     3781 atccccttcc gcacccgcct cggactctat tgggccccaa attctcacca gtccgtctcc
     3841 ttcaaaatcc attccaattc cacagccctt ccgaccagca gatgaagatc atcgaaatca
     3901 atttgggcaa cgagaccgat cctcatcagc tcccaatgtg catataaaca caatagaacc
     3961 tgtcaatatt gatgacttga ttagagacca aggatttcgt ggtgatggag gatcaaccac
     4021 aggtttgtct gctacccccc ctgcctcatt acctggctca ctaactaacg tgaaagcctt
     4081 acagaaatct ccaggacctc agcgagaaag gaagtcatct tcatcctcag aagacaggtc
     4141 aactttcttg tacaaagtgg gcggcgcaga aatcggtact ggctttccat tcgaccccca
     4201 ttatgtggaa gtcctgggcg agcgcatgca ctacgtcgat gttggtccgc gcgatggcac
     4261 ccctgtgctg ttcctgcacg gtaacccgac ctcctcctac gtgtggcgca acatcatccc
     4321 gcatgttgca ccgacccatc gctgcattgc tccagacctg atcggtatgg gcaaatccga
     4381 caaaccagac ctgggttatt tcttcgacga ccacgtccgc ttcatggatg ccttcatcga
     4441 agccctgggt ctggaagagg tcgtcctggt cattcacgac tggggctccg ctctgggttt
     4501 ccactgggcc aagcgcaatc cagagcgcgt caaaggtatt gcatttatgg agttcatccg
     4561 ccctatcccg acctgggacg aatggccaga atttgcccgc gagaccttcc aggccttccg
     4621 caccaccgac gtcggccgca agctgatcat cgatcagaac gtttttatcg agggtacgct
     4681 gccgatgggt gtcgtccgcc cgctgactga agtcgagatg gaccattacc gcgagccgtt
     4741 cctgaatcct gttgaccgcg agccactgtg gcgcttccca aacgagctgc caatcgccgg
     4801 tgagccagcg aacatcgtcg cgctggtcga agaatacatg gactggctgc accagtcccc
     4861 tgtcccgaag ctgctgttct ggggcacccc aggcgttctg atcccaccgg ccgaagccgc
     4921 tcgcctggcc aaaagcctgc ctaactgcaa ggctgtggac atcggcccgg gtctgaatct
     4981 gctgcaagaa gacaacccgg acctgatcgg cagcgagatc gcgcgctggc tgtcgacgct
     5041 cgagatttcc ggcgagccaa ccactgagga tctgtacttt cagagcgata acgcgatcgc
     5101 ctaataatca actttattat acatagttga taattctgca gatatccatc acactggcgg
     5161 ccgctcgagc atgcatctag agggccctat tctatagtgt cacctaaatg ctagagctcg
     5221 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt
     5281 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat
     5341 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag
     5401 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc
     5461 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg
     5521 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5581 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5701 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5821 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5881 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg
     5941 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6001 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccctagg ctccccagca
     6061 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6121 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6181 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     6241 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc
     6301 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
     6361 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     6421 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     6481 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg
     6541 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     6601 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     6661 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     6721 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
     6781 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     6841 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg
     6901 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     6961 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     7021 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     7081 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
     7141 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     7201 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7261 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7321 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7381 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7441 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7501 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7561 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7621 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7681 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7741 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7801 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7861 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7921 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7981 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8041 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8101 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8161 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8221 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8281 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8341 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8401 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8461 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8521 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8581 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8641 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8701 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8761 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8821 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8881 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8941 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9001 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9061 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9121 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9181 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9241 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9301 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9361 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9421 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9481 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9541 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9601 ttccgcgcac atttccccga aaagtgccac ct
//
