LOCUS       sequence-401170-        9796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter 
            driving the expression of Fzd1-mApple for zebrafish transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9796)
  AUTHORS   Veerapathiran S, Teh C, Zhu S, Kartigayen I, Korzh V, Matsudaira PT,
            Wohland T
  TITLE     Wnt3 distribution in the zebrafish brain is determined by 
            expression, diffusion and multiple molecular interactions.
  JOURNAL   Elife. 2020 Nov 25;9:e59489. doi: 10.7554/eLife.59489.
  PUBMED    33236989
REFERENCE   2  (bases 1 to 9796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-25"; volume: "9:e59489. doi"; pages: " 
            10.7554/eLife.59489"
COMMENT     Sequence Label: pminiTol2-4kb wnt3 promoter-Fzd1mApple
FEATURES             Location/Qualifiers
     source          1..9796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     150..167
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    284..305
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(320..325,326..343,344..350)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    358..374
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     363..385
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     382..398
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     382..398
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        416..434
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     416..433
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    join(485..496,497..659)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     CDS             6346..7056
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     6759..6778
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(7141..7328,7329..7340)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7352..7371)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7353..7371)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7378..7395)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7378..7394)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7387..7409)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7535..7990
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7622..7641)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7832..7853
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8068..8172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8173..8241,8242..9033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8391..8410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9204..9792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9693..9712
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
       61 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      121 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      181 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      241 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      301 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      361 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctattta
      421 ggtgacacta tagaatactc aagctatgca tccaacgcgt tgggagctct cccatatggt
      481 cgagcagagg tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca agtacttagg
      541 gaaaatttta ctcaattaaa agtaaaagta tctggctaga atcttacttg agtaaaagta
      601 aaaaagtact ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa aagcaagaaa
      661 gaaaactaga gattcttgtt taagcttcaa taaaagaagt gtctgttgtg gggccgaggg
      721 tcctgggggg ctgcggagct ttaggcattg tcctggaggg tgattgagag cgagaggagg
      781 ggagccgaga agcattgtgc gaggacggag cctttctaaa ggtggttatt aggagagaaa
      841 tggtgtgggg gtggctgttg actcttcatc ttgtgtgaac aaagtatctg gcgtatagct
      901 ttctctctct ctctctcgct ctcttgcggc tttcgcttta cgacggccat gaatactctc
      961 ggcgggctcg cccagacctg cgcgaaggct tattgtccgg cttaagtgcg gtttaattaa
     1021 gcgagctctg tgtcctgaga gaagagagga gctttagagc gctcgggtcc tgcggagatg
     1081 cagaggatga tgcgtaatat cagggctatt gaaggggaaa gtgtttgcgg ctctaatgaa
     1141 aaaggtttgt ttggggggga tgagtgggga ggggattgag gagtgtctgg cctttgtctc
     1201 cagcttacaa agtagggaca aaagcagtga tattatacaa atgcacccat ttccaggaca
     1261 tgatgcgagg acgggggcct ggagatgggt cggactgctc catctcagcc ccgttggcac
     1321 cttacaaaat gattttttcc ccccaccaca aagataggca ttggggataa accccgagag
     1381 ctggggcttc tgggtaccgc aaatttgggg gttgtggact taagcggatt ttctctgcaa
     1441 ctgtaagaac aagctaattc ctcagacctc ctttatctct aacatggtaa taacaaacaa
     1501 tgaaattctg aagagaaagg ggcaaaggaa gaggagggag gagagacgca gacacaaaag
     1561 aaggagagat ggagggaatg aaagagagga gagacgtgtg tgaggagagg gtgggcttgg
     1621 gcgtgttaag aaactttctc gctctagtgt tatgtgtgaa cactctgcta atccaggttt
     1681 tattgcttgc ctgtgtccgg ccacactgtc cggattgagt gtgtgtcagg gatagtgggg
     1741 gtctacacat gtccctcttt gaggaaagcc tgagaaagag ccggacctgg ttacggccgt
     1801 tttggggtcg cctaagaaac ctgcaccaca ggggtcctct gtgtatgtct ctgaattcaa
     1861 cgtgcatgtt gctagcaaac acatacggat taatgctaat gatgaaggtg tggctatatt
     1921 aggtacaaaa aaaaactttg acaatagttg tataaactcc tgtcaacagt ccatcaatgt
     1981 atgaagcaga actcgctttg aaaccaaaca ttgttgtgtg ggtccatttg gaccagttta
     2041 aggaaatctt ttgccctata aattccagcc tgcatgaatt tttatgatta ttgctcataa
     2101 acatactttt aaaaatgtgc attttaacta actatcaact tgtgcccgtc cactcaaagg
     2161 aagccaaaag ggtgtctctc ccagatcaca gggactacct tgtgggtgtg tagggtgatg
     2221 tattagctag gcgctaataa aatgcaggag tgtccacaaa catgacttaa tgagggagga
     2281 tgagagactc ctgacccaga ggtcaaagct ccgggcgact ctctttattc ccattgatct
     2341 gctggtgcac ttcatttcca tgctctaggg cccacttcag taaattcatt aagtgcgatt
     2401 gatacgagaa tcaagacttc ctccaaaaca caatggctat gctaggaggg aatggtcttg
     2461 tttgtgctgc agctatgtca agcatctctg gcttttgtcc tggtttcgca ccattgcggt
     2521 gcatgctggg gtttgagtcc tcgagattct aatcgtaagc tcatttccag ctggttcgtt
     2581 gtgttgcgcc cctatggcct tgacctggga aacgtggttt cagtgttgtt gagatgccga
     2641 ccgcagaccg ttccttgcct ccactcgtaa attacgctcc gatcaggttt tcccagccaa
     2701 tcctggcgag cagatctgca tctgtcactc tgcaagaatg ctgaatcaaa ccgctgatct
     2761 aggcacaata cagctgatgg aaagcagatc tttattccaa catcaggaac gtagcacgag
     2821 ccgagtgctt ctggaagctg caatttaacc aacaggttca tatctgatga gatcaaggat
     2881 gcatcatcct ggaagaaaca ctgtgatgaa catttaagat gtcagaatga cccgtcatgc
     2941 ctctgagctg agtccccgca cagcgcttat tcttttgaag agggagaata aaagcaaaaa
     3001 aacagaggcg atggaaatat gacatccaga ccgcggtcag aactgtgagt gtctctcctg
     3061 ggctcctatg gttgtggtgc acagtccatg tccctgctat gagttagata gacagatgca
     3121 ctggcagata gacagacaga caggctttac tacttggtcg ctggattgca ggaaagagct
     3181 ttatccaacc agagtcaagc aagcaacatg ggacaataac ataattaaat attctgggga
     3241 aaagtggcac ttatgcaaat gttttttgta atgggttgga gaaaattggc cggaacctac
     3301 ataattttta atgacaggat gattcatttg tgaaattgta attcaaagaa attcagtaaa
     3361 ctaactaaca atctaaaaaa ttctttgatt tgcagtttaa ttctacttcc taacattgat
     3421 ttgtttcaac tttattcaaa tggcaaaact aaattagtac tcaaagctcc aaatgatatg
     3481 attcctagac tgtttaaagt gctaaaagtg ccaaaactta cagctaccca catgaactta
     3541 tatttcattc tgcctccatt tgcaattcct ccagtgtttg tacctgaaac tgttgtcagt
     3601 tctcacctct gtcctgccgt tcattctgac acagagactc tatatttgaa tgtattgtca
     3661 acttctaatg ccgtgtctac tactctgtgc tgtctgctct catcccatcc gaatgacaat
     3721 agtgtttatg taaaatgtgc tgcctttggg ccgctggggc acagaacact gcatgcctgg
     3781 ccctgccaca ccacctttta cggcttttta ttgtgttgcc tttgtgtcac aaggggagga
     3841 cgcggccatc aaaattctcc aagacaaaat tcttgaatgt atgtgcacgc acacgcatcc
     3901 ggaacaatta gttcaggagc acgggtgctt aaaaaacgga gacgacactt ctcgccacca
     3961 cctcctcgcc gagcagcagg cccgagtctg gcacagctgg gtttccttga tgctaacgca
     4021 gacaatatag atcttaataa gacaaatcta cctctccgaa actcagtcct gacacccttt
     4081 cattcttatt ccctcccagc ctcctctctc tctctctctc tctttctttc tttctttgct
     4141 acattaccag ggggtggtgc atgattgaca gatgggcagg aataataatt tttttttttt
     4201 ttttgcgtaa cctaaggcca tgctctgtga ttggcttgcc agctgacatc tccaaacctc
     4261 tcttgtcgtt tttttcccct ctctctctct cgctttctct ctctcactct ctctctctaa
     4321 tattggccct gatgatccta aggggaaaaa agtggggaga gaaaaagata tgtaccttag
     4381 ggaagaacct cagtaagagg gagggaatca gaggacgagt aggagcgtgt gtgcgagtgt
     4441 gtgaaagaga cacttactct cctaactact actggaagac acacactttt ctcacccaac
     4501 tcaaagcagc agcaaacctt ggagaattgt tttatttttg ttttttcgcg aaccccccta
     4561 aaaaaattcc taattattct ctcaagcatc catctttgcc ttgccgggat ttaattttca
     4621 ccccctctct aggaatccta tgtactgatc aatagaccct ttccatccac acaagacata
     4681 cctcgtttct caccgcacac cgaaagtatg gccgcgcgcg cgctcttcct gatcgccctc
     4741 ggagctctga gcgcccgcgc gcagtacgga gaccgcggaa tgtcggtgcc cgagcatggc
     4801 ttctgccagc ccatctccat ccctctgtgc acggacatcg cctacaacga gaccatcatg
     4861 cccaacctgc tcggccacac caaccaggag gacgcgggac tggaagtgca ccagttttac
     4921 ccgctggtga aagtgcagtg ctcgccagac ctcaagttct tcctgtgctc catgtacgcg
     4981 ccggtgtgca cggtgctgga gcaggcgctg ccgccgtgcc ggtccctctg cgagcgcgcg
     5041 cgtcagggct gcgaggcgct gatgaataaa ttcggcttcc agtggccgga gagcctggcg
     5101 tgcgagtcct tcccggtgca cggcggagag ctctgcgtgg gacagaacac caccgggccg
     5161 agccccccag tcagcccgac tcccgacgcg acgcaggcgc ctccggagcc ctcccagcgc
     5221 ggccgcttcc agtgcccgcc gtcgctcaga gtccccccgt acctcaacta ccgcttcatg
     5281 ggagaggaca actgcggagc gccctgcgag cccagaaaac tgcacggcat gatgtacttc
     5341 agcgaggacg agcagaagtt tgcgcgcatc tggatcggga tctggtccgt cctatgctgc
     5401 gcttcaactt tattcacagt tctgacttat ctggtggaca tgaagcgctt cagctacccg
     5461 gagagaccca tcatattcct gtccggctgc tacaccatgg tgtccatcgc ctacatcgcc
     5521 ggatttctcc tggaggacaa ggttgtctgc aacgagcagt tcgagaacga gttcaggact
     5581 gtggttcagg gcaccaagaa ggaaggctgc accatattgt tcatgatgct gtacttcttc
     5641 agcatgtcca gctccatctg gtgggtgatc ctggcgctca cctggttcct ggcggccggg
     5701 atgaaatggg gtcacgaggc catcgaggcc aactcgcagt acttccacct cgcggcctgg
     5761 gcagtgcccg ccatcaaaac catcaccatc ctggctgtgg gtcaggtgga cggggacgtg
     5821 ctcagcgggg tgtgctttgt gggcatcaac agcgtggacg cgctgcgcgg attcgtgctc
     5881 gcgccgctct tcgtgtacct gttcatcggc acctctttcc tcctggcggg attcgtgtcg
     5941 ttgttccgca tcaggaccat catgaagcac gacggcacta aaacggagaa gctggagaag
     6001 cttatggtgc ggatcgggat cttcagcgtg ctctacacgg ttccggccac catcgtgatc
     6061 gcctgttact tctacgagca ggccttccgc gcgcactggg agcagacgtg gagcgcgcac
     6121 tcgtgcaaga catacgcggt gccgtgtccc gcgcacaacc cgcagatgaa cccggacttc
     6181 acggtgttca tgatcaagta cctgatgacc ctgatcgtgg gcatcacctc cggcttctgg
     6241 atatggtctg gaaagacgct caattcctgg aggaagtttt acacgagact ggcgaacagc
     6301 aaacagggag aaaccacagt gggaggagga tcaggaggag gatcaatggt gagcaagggc
     6361 gaggagaata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     6421 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgaggcc
     6481 tttcagaccg ctaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     6541 ctgtcccctc agttcatgta cggctccaag gtctacatta agcacccagc cgacatcccc
     6601 gactacttca agctgtcctt ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag
     6661 gacggcggca ttattcacgt taaccaggac tcctccctgc aggacggcgt gttcatctac
     6721 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     6781 atgggctggg aggcctccga ggagcggatg taccccgagg acggcgccct gaagagcgag
     6841 atcaagaaga ggctgaagct gaaggacggc ggccactacg ccgccgaggt caagaccacc
     6901 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca tcgtcgacat caagttggac
     6961 atcgtgtccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     7021 cactccaccg gcggcatgga cgagctgtac aagtaagctc tagatggcca gatctattta
     7081 aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg
     7141 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag
     7201 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta
     7261 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac
     7321 tcaagtactt tacacctctg ggcccaattc gccctatagt gagtcgtatt acaattcact
     7381 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7441 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7501 ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc cctgtagcgg cgcattaagc
     7561 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7621 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7681 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7741 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7801 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7861 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7921 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7981 cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8041 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8101 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8161 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8221 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8281 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8341 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8401 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8461 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8521 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8581 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     8641 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     8701 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     8761 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8821 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8881 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8941 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9001 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9061 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9121 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9181 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     9241 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     9301 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     9361 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     9421 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     9481 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     9541 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9601 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9661 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9721 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9781 agcctatgga aaaacg
//
