LOCUS       sequence-401182-        8527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, 
            Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Casas-Mollano JA, Zinselmeier MH, Sychla A, Smanski MJ
  TITLE     Efficient gene activation in plants by the MoonTag programmable 
            transcriptional activator.
  JOURNAL   Nucleic Acids Res. 2023 Jul 21;51(13):7083-7093. doi: 
            10.1093/nar/gkad458.
  PUBMED    37254802
REFERENCE   2  (bases 1 to 8527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-21- 21"; volume: "51"; issue: 
            "13"; pages: "7083-7093"
COMMENT     Sequence Label: pMOD_A-CmYLCV-dCas9-24XGP41
FEATURES             Location/Qualifiers
     source          1..8527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        67..171
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1239..1827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1728..1747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1981..1998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(2514..2540,2541..2543,2544..5030,5031..5033,
                     5034..6617)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             2514..6617
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6621..6647
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6627..6653
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             6666..6686
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6693..6713
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6723..6743
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6810..6854
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             6870..6914
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             6930..6974
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             6990..7034
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7050..7094
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7110..7154
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7170..7214
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7230..7274
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7290..7334
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7350..7394
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7410..7454
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7470..7514
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7530..7574
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7590..7634
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7650..7694
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7710..7754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7770..7814
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7830..7874
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7890..7934
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7950..7994
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8010..8054
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8070..8114
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8130..8174
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8190..8234
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     terminator      8275..8523
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
ORIGIN
        1 gagctcggac ttttgcaggt gtgatgaaaa gccttagggt caggtggcac ttttcgggga
       61 aatgtgcgat gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgcta
      121 atgagacaat aaccctgata aatgcttcaa ttatattgaa aaaggaagag tatgagtatc
      181 caacatttcc gtgtcgccct tattcccttt tttgcggcct tctgccttcc tgtttttgct
      241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc ccgagtgggt
      301 tacattgaac tggatctcaa cagcggcaaa atcttagaga gttttcgccc cgaagaacgc
      361 ttcccaatga tgagcacttt caaagttctg ctatgtggcg cggtgttatc ccgtattgat
      421 gctgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtat
      481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgtagtgct
      541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat tggaggaccg
      601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      721 atggcaacaa ctctccgcaa actattaact ggcgaactac ttactcttgc ttcacgccag
      781 caactcattg actggatgga ggcggacaaa gttgcaggac cacttctgcg ctcggcactt
      841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc tcgcggtatc
      901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
      961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1021 aagcattggt aactgtcaga cgaagggaaa taaatagtag cccgcctgat gtgcgggctt
     1081 ttttttggtg gtggttacca gtgtcgctta gctaccgtag gtcctcttag gcggtccgat
     1141 tagcgacata gtcatcaatg ccttgacggc aaaatccctt aacgtgagtt ttcgttccac
     1201 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1261 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1321 caagagctac taactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1381 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1441 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1501 cttaccgggt tggactaaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1561 gggggttcgt gctcacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1621 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1681 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1741 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1801 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1861 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1921 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1981 agcgagtcag tgagcgagga agcggaagag cgcccacctg cttttgatcg gcgcgccgga
     2041 gtggcagaca tactgtccca caaatgaaga tggaatctgt aaaagaaaac gcgtgaaata
     2101 atgcgtctga caaaggttag gtcggctgcc tttaatcaat accaaagtgg tccctaccac
     2161 gatggaaaaa ctgtgcagtc ggtttggctt tttctgacga acaaataaga ttcgtggccg
     2221 acaggtgggg gtccaccatg tgaaggcatc ttcagactcc aataatggag caatgacgta
     2281 agggcttacg aaataagtaa gggtagtttg ggaaatgtcc actcacccgt cagtctataa
     2341 atacttagcc cctccctcat tgttaaggga gcaaaatctc agagagatag tcctagagag
     2401 agaaagagag caagtagcct agaagtagtc aaggcggcga agtattcagg cacgtggcca
     2461 ggaagaagaa aagccaagac gacgaaaaca ggtaagagct aagcttaatg aacatggaca
     2521 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg
     2581 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     2641 tcaagaagaa cctgatcggc gccctgctgt tcgacagcgg agaaacagcc gaggccaccc
     2701 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     2761 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     2821 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     2881 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     2941 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     3001 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     3061 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     3121 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     3181 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc aacctgattg
     3241 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     3301 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     3361 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     3421 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     3481 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     3541 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     3601 tcgatggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     3661 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     3721 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     3781 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     3841 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     3901 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     3961 acaagggcgc cagcgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     4021 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtacaacg
     4081 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     4141 agcagaaaaa agccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     4201 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     4261 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     4321 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     4381 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     4441 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     4501 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     4561 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     4621 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     4681 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     4741 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     4801 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     4861 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     4921 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     4981 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac gctatcgtgc
     5041 ctcagagctt tctgaaggac gactccatcg ataacaaagt gctgactcgg agcgacaaga
     5101 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     5161 ggcgccagct gctgaatgcc aagctgatta cccagaggaa gttcgacaat ctgaccaagg
     5221 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     5281 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     5341 acgacgagaa cgacaaactg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     5401 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     5461 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     5521 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     5581 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     5641 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     5701 tcgagacaaa cggcgaaaca ggcgagatcg tgtgggataa gggccgggac tttgccaccg
     5761 tgcggaaagt gctgtctatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     5821 gcggcttcag caaagagtct atcctgccca agaggaacag cgacaagctg atcgccagaa
     5881 agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg
     5941 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     6001 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     6061 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     6121 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg
     6181 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     6241 agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca
     6301 aacactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg
     6361 ccgacgctaa tctggacaag gtgctgagcg cctacaacaa gcacagagac aagcctatca
     6421 gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg
     6481 ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc
     6541 tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt
     6601 ctcagctggg aggcgacgcc tatccctatg acgtgcccga ttatgccagc ctgggcagcg
     6661 gctcccccaa gaaaaaacgc aaggtggaag atcctaagaa aaagcggaaa gtggaagatg
     6721 ctccaaagaa gaagagaaag gtcgacggca ttggtagtgg gagcaacggc agcagcggat
     6781 cccttaagct tgccaccatg cgtacgggca agaatgagca ggaactgctg gaactggaca
     6841 aatgggcaag cctggggagc ggctccggaa agaacgagca ggaactgctg gaactggaca
     6901 agtgggcttc tctgggctcc gggtccggga aaaacgaaca ggagctgctg gagctggata
     6961 agtgggccag cctgggctcc gggtccggga agaatgaaca ggaactgctg gaactggata
     7021 aatgggcatc tctgggcagc gggtccggaa agaatgaaca ggagctgctg gaactggaca
     7081 agtgggcatc cctggggagc gggtccggaa agaatgaaca ggaactgctg gaactggata
     7141 agtgggcatc cctgggaagc ggctccggaa aaaacgagca ggagctgctg gagctggaca
     7201 agtgggcctc tctgggctcc ggctccggga agaatgaaca ggagctgctg gaactggata
     7261 agtgggcatc tctggggtcc ggaagcggta aaaacgagca ggaactgctg gaactggaca
     7321 agtgggcaag cctggggtct ggctccggca aaaacgaaca ggaactgctg gaactggaca
     7381 aatgggccag cctgggctcc gggtccggga aaaatgagca ggaactgctg gaactggata
     7441 aatgggcgag cctgggcagc gggtccggaa aaaacgaaca ggagctgctg gagctggaca
     7501 aatgggcgag tctgggctcc ggaagcggca agaatgaaca ggaactgctg gagctggaca
     7561 aatgggccag cctgggcagc ggctccggaa agaatgagca ggaactgctg gagctggaca
     7621 aatgggcatc tctgggctcc ggctccggga aaaacgagca ggaactgctg gagctggaca
     7681 agtgggcttc cctgggcagc ggctccggaa aaaatgaaca ggaactgctg gaactggaca
     7741 agtgggccag cctggggtcc gggtctggga agaacgagca ggaactgctg gagctggaca
     7801 agtgggcatc tctggggtct ggctctggca agaacgagca ggaactgctg gaactggaca
     7861 agtgggcgag cctgggcagc gggtctggca agaatgaaca ggagctgctg gaactggaca
     7921 aatgggccag cctggggtct gggtctggaa agaatgagca ggaactgctg gagctggaca
     7981 agtgggcatc tctgggctct gggtctggca agaacgagca ggagctgctg gagctggata
     8041 agtgggcatc cctggggtcc gggtccggca agaatgaaca ggagctgctg gagctggata
     8101 aatgggcatc cctgggctct gggtctggga agaatgagca ggagctgctg gaactggata
     8161 agtgggcgag cctgggcagc gggtctggca agaatgagca ggaactgctg gagctggaca
     8221 agtgggcttc tctgcaccgg tcagcagtgg aggacagcac gtaacccggg cttatatgaa
     8281 gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt
     8341 tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat
     8401 cacattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc
     8461 ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat
     8521 aagcgct
//
