LOCUS       sequence-401186-        9389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Moclo level 1 - Module A, Promoter: AtUBI10, Gene: dCas9-24XGP41, 
            Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9389)
  AUTHORS   Casas-Mollano JA, Zinselmeier MH, Sychla A, Smanski MJ
  TITLE     Efficient gene activation in plants by the MoonTag programmable 
            transcriptional activator.
  JOURNAL   Nucleic Acids Res. 2023 Jul 21;51(13):7083-7093. doi: 
            10.1093/nar/gkad458.
  PUBMED    37254802
REFERENCE   2  (bases 1 to 9389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-21- 21"; volume: "51"; issue: 
            "13"; pages: "7083-7093"
COMMENT     Sequence Label: pMOD_A-AtUBI10-dCas9-24XGP41
FEATURES             Location/Qualifiers
     source          1..9389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        67..171
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1239..1827
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1728..1747
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1981..1998
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(3376..3402,3403..3405,3406..5892,5893..5895,
                     5896..7479)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3376..7479
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7483..7509
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7489..7515
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7528..7548
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7555..7575
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7585..7605
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7672..7716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7732..7776
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7792..7836
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7852..7896
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7912..7956
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7972..8016
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8032..8076
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8092..8136
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8152..8196
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8212..8256
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8272..8316
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8332..8376
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8392..8436
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8452..8496
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8512..8556
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8572..8616
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8632..8676
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8692..8736
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8752..8796
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8812..8856
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8872..8916
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8932..8976
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             8992..9036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9052..9096
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     terminator      9137..9385
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
ORIGIN
        1 gagctcggac ttttgcaggt gtgatgaaaa gccttagggt caggtggcac ttttcgggga
       61 aatgtgcgat gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgcta
      121 atgagacaat aaccctgata aatgcttcaa ttatattgaa aaaggaagag tatgagtatc
      181 caacatttcc gtgtcgccct tattcccttt tttgcggcct tctgccttcc tgtttttgct
      241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc ccgagtgggt
      301 tacattgaac tggatctcaa cagcggcaaa atcttagaga gttttcgccc cgaagaacgc
      361 ttcccaatga tgagcacttt caaagttctg ctatgtggcg cggtgttatc ccgtattgat
      421 gctgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtat
      481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgtagtgct
      541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat tggaggaccg
      601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      721 atggcaacaa ctctccgcaa actattaact ggcgaactac ttactcttgc ttcacgccag
      781 caactcattg actggatgga ggcggacaaa gttgcaggac cacttctgcg ctcggcactt
      841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtggctc tcgcggtatc
      901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
      961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1021 aagcattggt aactgtcaga cgaagggaaa taaatagtag cccgcctgat gtgcgggctt
     1081 ttttttggtg gtggttacca gtgtcgctta gctaccgtag gtcctcttag gcggtccgat
     1141 tagcgacata gtcatcaatg ccttgacggc aaaatccctt aacgtgagtt ttcgttccac
     1201 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1261 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1321 caagagctac taactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1381 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1441 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1501 cttaccgggt tggactaaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1561 gggggttcgt gctcacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1621 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1681 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1741 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1801 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1861 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1921 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1981 agcgagtcag tgagcgagga agcggaagag cgcccacctg cttttgatcg gcgcgccgga
     2041 ggtcgagctg caggtcaacg gatcaggata ttcttgttta agatgttgaa ctctatggag
     2101 gtttgtatga actgatgatc taggaccgga taagttccct tcttcatagc gaacttattc
     2161 aaagaatgtt ttgtgtatca ttcttgttac attgttatta atgaaaaaat attattggtc
     2221 attggactga acacgagtgt taaatatgga ccaggcccca aataagatcc attgatatat
     2281 gaattaaata acaagaataa atcgagtcac caaaccactt gcctttttta acgagacttg
     2341 ttcaccaact tgatacaaaa gtcattatcc tatgcaaatc aataatcata caaaaatatc
     2401 caataacact aaaaaattaa aagaaatgga taatttcaca atatgttata cgataaagaa
     2461 gttacttttc caagaaattc actgatttta taagcccact tgcattagat aaatggcaaa
     2521 aaaaaacaaa aaggaaaaga aataaagcac gaagaattct agaaaatacg aaatacgctt
     2581 caatgcagtg ggacccacgg ttcaattatt gccaattttc agctccaccg tatatttaaa
     2641 aaataaaacg ataatgctaa aaaaatataa atcgtaacga tcgttaaatc tcaacggctg
     2701 gatcttatga cgaccgttag aaattgtggt tgtcgacgag tcagtaataa acggcgtcaa
     2761 agtggttgca gccggcacac acgagtcgtg tttatcaact caaagcacaa atacttttcc
     2821 tcaacctaaa aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac
     2881 acgtgtcatt ttattattag ctattgcttc accgccttag ctttctcgtg acctagtcgt
     2941 cctcgtcttt tcttcttctt cttctataaa acaataccca aagagctctt cttcttcaca
     3001 attcagattt caatttctca aaatcttaaa aactttctct caattctctc taccgtgatc
     3061 aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt cgttcgatcc
     3121 caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgaac acgattttct
     3181 gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat atcatccgat
     3241 ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat ttctatctag
     3301 atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga gtttttctga
     3361 ttaacaggaa tgaacatgga caagaagtac agcatcggcc tggccatcgg caccaactct
     3421 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
     3481 ggcaacaccg accggcacag catcaagaag aacctgatcg gcgccctgct gttcgacagc
     3541 ggagaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
     3601 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
     3661 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
     3721 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
     3781 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
     3841 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
     3901 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
     3961 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
     4021 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
     4081 ggcctgttcg gcaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
     4141 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
     4201 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
     4261 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
     4321 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
     4381 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
     4441 aagaacggct acgccggcta catcgatggc ggagccagcc aggaagagtt ctacaagttc
     4501 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga
     4561 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
     4621 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
     4681 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
     4741 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
     4801 tggaacttcg aggaagtggt ggacaagggc gccagcgccc agagcttcat cgagcggatg
     4861 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
     4921 gagtacttca ccgtgtacaa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
     4981 aagcccgcct tcctgagcgg cgagcagaaa aaagccatcg tggacctgct gttcaagacc
     5041 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
     5101 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
     5161 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
     5221 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
     5281 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
     5341 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
     5401 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
     5461 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
     5521 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     5581 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     5641 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     5701 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     5761 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     5821 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     5881 tacgatgtgg acgctatcgt gcctcagagc tttctgaagg acgactccat cgataacaaa
     5941 gtgctgactc ggagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
     6001 gtgaagaaga tgaagaacta ctggcgccag ctgctgaatg ccaagctgat tacccagagg
     6061 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     6121 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     6181 gactcccgga tgaacactaa gtacgacgag aacgacaaac tgatccggga agtgaaagtg
     6241 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
     6301 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
     6361 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
     6421 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
     6481 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     6541 gagatccgga agcggcctct gatcgagaca aacggcgaaa caggcgagat cgtgtgggat
     6601 aagggccggg actttgccac cgtgcggaaa gtgctgtcta tgccccaagt gaatatcgtg
     6661 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
     6721 agcgacaagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
     6781 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
     6841 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
     6901 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
     6961 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
     7021 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
     7081 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
     7141 cagctgtttg tggaacagca caaacactac ctggacgaga tcatcgagca gatcagcgag
     7201 ttctccaaga gagtgatcct ggccgacgct aatctggaca aggtgctgag cgcctacaac
     7261 aagcacagag acaagcctat cagagagcag gccgagaata tcatccacct gtttaccctg
     7321 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
     7381 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
     7441 tacgagacac ggatcgacct gtctcagctg ggaggcgacg cctatcccta tgacgtgccc
     7501 gattatgcca gcctgggcag cggctccccc aagaaaaaac gcaaggtgga agatcctaag
     7561 aaaaagcgga aagtggaaga tgctccaaag aagaagagaa aggtcgacgg cattggtagt
     7621 gggagcaacg gcagcagcgg atcccttaag cttgccacca tgcgtacggg caagaatgag
     7681 caggaactgc tggaactgga caaatgggca agcctgggga gcggctccgg aaagaacgag
     7741 caggaactgc tggaactgga caagtgggct tctctgggct ccgggtccgg gaaaaacgaa
     7801 caggagctgc tggagctgga taagtgggcc agcctgggct ccgggtccgg gaagaatgaa
     7861 caggaactgc tggaactgga taaatgggca tctctgggca gcgggtccgg aaagaatgaa
     7921 caggagctgc tggaactgga caagtgggca tccctgggga gcgggtccgg aaagaatgaa
     7981 caggaactgc tggaactgga taagtgggca tccctgggaa gcggctccgg aaaaaacgag
     8041 caggagctgc tggagctgga caagtgggcc tctctgggct ccggctccgg gaagaatgaa
     8101 caggagctgc tggaactgga taagtgggca tctctggggt ccggaagcgg taaaaacgag
     8161 caggaactgc tggaactgga caagtgggca agcctggggt ctggctccgg caaaaacgaa
     8221 caggaactgc tggaactgga caaatgggcc agcctgggct ccgggtccgg gaaaaatgag
     8281 caggaactgc tggaactgga taaatgggcg agcctgggca gcgggtccgg aaaaaacgaa
     8341 caggagctgc tggagctgga caaatgggcg agtctgggct ccggaagcgg caagaatgaa
     8401 caggaactgc tggagctgga caaatgggcc agcctgggca gcggctccgg aaagaatgag
     8461 caggaactgc tggagctgga caaatgggca tctctgggct ccggctccgg gaaaaacgag
     8521 caggaactgc tggagctgga caagtgggct tccctgggca gcggctccgg aaaaaatgaa
     8581 caggaactgc tggaactgga caagtgggcc agcctggggt ccgggtctgg gaagaacgag
     8641 caggaactgc tggagctgga caagtgggca tctctggggt ctggctctgg caagaacgag
     8701 caggaactgc tggaactgga caagtgggcg agcctgggca gcgggtctgg caagaatgaa
     8761 caggagctgc tggaactgga caaatgggcc agcctggggt ctgggtctgg aaagaatgag
     8821 caggaactgc tggagctgga caagtgggca tctctgggct ctgggtctgg caagaacgag
     8881 caggagctgc tggagctgga taagtgggca tccctggggt ccgggtccgg caagaatgaa
     8941 caggagctgc tggagctgga taaatgggca tccctgggct ctgggtctgg gaagaatgag
     9001 caggagctgc tggaactgga taagtgggcg agcctgggca gcgggtctgg caagaatgag
     9061 caggaactgc tggagctgga caagtgggct tctctgcacc ggtcagcagt ggaggacagc
     9121 acgtaacccg ggcttatatg aagatgaaga tgaaatattt ggtgtgtcaa ataaaaagct
     9181 tgtgtgctta agtttgtgtt tttttcttgg cttgttgtgt tatgaatttg tggctttttc
     9241 taatattaaa tgaatgtaag atcacattat aatgaataaa caaatgtttc tataatccat
     9301 tgtgaatgtt ttgttggatc tcttctgcag catataacta ctgtatgtgc tatggtatgg
     9361 actatggaat atgattaaag ataagcgct
//
