LOCUS       sequence-401203-       19565 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for expression of the two protein components of the 
            MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the 
            control of the CmYLCV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19565)
  AUTHORS   Casas-Mollano JA, Zinselmeier MH, Sychla A, Smanski MJ
  TITLE     Efficient gene activation in plants by the MoonTag programmable 
            transcriptional activator.
  JOURNAL   Nucleic Acids Res. 2023 Jul 21;51(13):7083-7093. doi: 
            10.1093/nar/gkad458.
  PUBMED    37254802
REFERENCE   2  (bases 1 to 19565)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2023-07-21- 21"; volume: "51"; issue: 
            "13"; pages: "7083-7093"
COMMENT     Sequence Label: pMT1-24X-NOG(CmYLCV)
FEATURES             Location/Qualifiers
     source          1..19565
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1112..1136
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1214..1388
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1429..2454)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     protein_bind    4394..4415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4430..4435,4436..4453,4454..4460)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4468..4484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4473..4495
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4492..4508
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4492..4508
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(5027..5053,5054..5056,5057..7543,7544..7546,
                     7547..9130)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5027..9130
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9134..9160
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9140..9166
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             9179..9199
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9206..9226
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9236..9256
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9323..9367
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9383..9427
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9443..9487
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9503..9547
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9563..9607
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9623..9667
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9683..9727
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9743..9787
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9803..9847
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9863..9907
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9923..9967
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             9983..10027
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10043..10087
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10103..10147
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10163..10207
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10223..10267
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10283..10327
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10343..10387
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10403..10447
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10463..10507
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10523..10567
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10583..10627
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10643..10687
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             10703..10747
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     terminator      10788..11036
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             11984..12694
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             11984..12694
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(12011..12039)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     12634..12654
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             12734..12883
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             12908..12928
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12959..13120
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             13148..13198
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     primer_bind     complement(14178..14195)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14178..14194)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14187..14209)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    14397..14421
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             15722..16351
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             16780..17853
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      17919..18113
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(18349..18371)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    18457..18597
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     18512..18531
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(18612..18629)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(18783..19371)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18863..18882)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(19458..19565,1..687))
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
       61 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
      121 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
      181 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
      241 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
      301 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
      361 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
      421 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
      481 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
      541 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
      601 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
      661 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
      721 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
      781 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
      841 atagtatcga cggagccgat tttgaaaccg cggtgatcac aggcagcaac gctctgtcat
      901 cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc ggcagcttag
      961 ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt acaacggctc
     1021 tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
     1081 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     1141 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactga attaacgccg
     1201 aattaattcg ggggatctgg attttagtac tggattttgg ttttaggaat tagaaatttt
     1261 attgatagaa gtattttaca aatacaaata catactaagg gtttcttata tgctcaacac
     1321 atgagcgaaa ccctatagga accctaattc ccttatctgg gaactactca cacattatta
     1381 tggagaaact cgagcttgtc gatcgacaga tcccggtcgg catctactct atttctttgc
     1441 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     1501 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     1561 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     1621 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gtcgtggcga
     1681 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     1741 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     1801 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     1861 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     1921 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     1981 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     2041 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     2101 agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     2161 gacaccctgt gaacggcggg agatgcaata ggtcaggctc tcgctaaact ccccaatgtc
     2221 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     2281 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     2341 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa
     2401 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcataagctt
     2461 ctgcaaaaga gaaccagaca acagggtaag tgcctagcag taaacaaaca gaactcatca
     2521 caagcaaaca gcaacatcat attcatacca acaggtcatg tgtgttcatc acatcattag
     2581 tactaagcat gccatcatcc aagtatatca aagtaagggc aaagagcatg catgatcatc
     2641 aggtgcacaa aagaatcatc aaattgtagc agtacatatc ttcatctatc atgcatatct
     2701 atccataaca ggacgatgca tgttgaccag gtaaaagcta caggatccta taggaacagc
     2761 agcgtatata tcttcaccaa tcttagcatc ttaatcatgt ggcacatgca gtttcaattt
     2821 aagcacatga gctagttgat tatgaggtac cagagaatca tcaaactgat gtagcagcat
     2881 atatcctcat ctatcatgca atctaatcta atctaactaa acaggaaagg tgtgctattc
     2941 agttaaaagc taccgcatca tatacaaacg gcagcataag aaaaagcata atcatcttaa
     3001 tcatgcaaca aacgcagatt cataataagc ccaagagcta gcttgtgatg atcttattct
     3061 actctgatct acagcaatca gataacgacc taaccttgca catggcaaca aaacaatcga
     3121 tcggacgaat cagttgtttg ttcctagcta gcaccatcga accagataat agatgcacgt
     3181 acagatcccg aaaacgaacc caaaaacagg gcagacctag ctgaacctag gcagcgaccc
     3241 agcagatcgt gagaacgatc tcatctacga acagcctaga agcaacccca cgattcccgg
     3301 acaaacgacc taaaatcccc acaaatcaca tgagcatgac aggataaaca gcggaaccga
     3361 tcagatctac acgaaaaccc cacctcccag ccacccacga tcaggaaaca cgcggatcta
     3421 gcatgatttc gtcaacgcct cagcctagtt cctagccaca gaccaagcag aaccaccaaa
     3481 ccacgccgag cgaggagatg gggcaagagg acgggggaga cgatcgccgt accttgaagc
     3541 gggggaagga tcgccgaggg tcgcgaggag agcaatttgg atttggaacc cgggggttgt
     3601 gcgctccgaa cgatgagacg atgtgagatt gtgggaagag gcgcggaggg ccctgtattt
     3661 atgggctgcg acggggggag gagaggtggg gagggttggg gaaggaatcc cccacccgtg
     3721 ccgtgacggt tccgggccgt gtgagaggag cccgctcgtc tccgccacgc aatttccgcg
     3781 atcggagcgg agctttcgag aggcggctgg atggttggtg gccgttagat ttgtagacgc
     3841 cgttaacgcc tcgcctccac cgggaagagt tttgagcagc cgcttatgac aatggcttaa
     3901 cgacgttaga cggagcgtta gtggcaggcc atgatagaat agacgtattg caatgggatt
     3961 ataattaaat aaataagaat ataataagat atggcaagtc ggcactcatg acatggtctt
     4021 cgaaatgata gtgctcactt tcttagccga gaaagttgac gcgactgatt tagaagttaa
     4081 gatattattt ctctcttctt ttctttcctc gtcatataag gatgaaataa actttagaga
     4141 ttgccggtag tgattttgga tttcggcgat caggcttggt ttgccggttt cggacggtgt
     4201 gccttaggcc acccgcagtg tatcttgtaa tgttcaaccg ataagcaagg gtggggctca
     4261 agcaagtagt aaacaactat gtcaaatgtc accatggtta tggtcttgtt tagttggctt
     4321 cttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4381 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4441 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4501 gctatgacat gattacgaat tcgagctcgg tacccgggga tcggcgcgcc ggagtggcag
     4561 acatactgtc ccacaaatga agatggaatc tgtaaaagaa aacgcgtgaa ataatgcgtc
     4621 tgacaaaggt taggtcggct gcctttaatc aataccaaag tggtccctac cacgatggaa
     4681 aaactgtgca gtcggtttgg ctttttctga cgaacaaata agattcgtgg ccgacaggtg
     4741 ggggtccacc atgtgaaggc atcttcagac tccaataatg gagcaatgac gtaagggctt
     4801 acgaaataag taagggtagt ttgggaaatg tccactcacc cgtcagtcta taaatactta
     4861 gcccctccct cattgttaag ggagcaaaat ctcagagaga tagtcctaga gagagaaaga
     4921 gagcaagtag cctagaagta gtcaaggcgg cgaagtattc aggcacgtgg ccaggaagaa
     4981 gaaaagccaa gacgacgaaa acaggtaaga gctaagctta atgaacatgg acaagaagta
     5041 cagcatcggc ctggccatcg gcaccaactc tgtgggctgg gccgtgatca ccgacgagta
     5101 caaggtgccc agcaagaaat tcaaggtgct gggcaacacc gaccggcaca gcatcaagaa
     5161 gaacctgatc ggcgccctgc tgttcgacag cggagaaaca gccgaggcca cccggctgaa
     5221 gagaaccgcc agaagaagat acaccagacg gaagaaccgg atctgctatc tgcaagagat
     5281 cttcagcaac gagatggcca aggtggacga cagcttcttc cacagactgg aagagtcctt
     5341 cctggtggaa gaggataaga agcacgagcg gcaccccatc ttcggcaaca tcgtggacga
     5401 ggtggcctac cacgagaagt accccaccat ctaccacctg agaaagaaac tggtggacag
     5461 caccgacaag gccgacctgc ggctgatcta tctggccctg gcccacatga tcaagttccg
     5521 gggccacttc ctgatcgagg gcgacctgaa ccccgacaac agcgacgtgg acaagctgtt
     5581 catccagctg gtgcagacct acaaccagct gttcgaggaa aaccccatca acgccagcgg
     5641 cgtggacgcc aaggccatcc tgtctgccag actgagcaag agcagacggc tggaaaatct
     5701 gatcgcccag ctgcccggcg agaagaagaa tggcctgttc ggcaacctga ttgccctgag
     5761 cctgggcctg acccccaact tcaagagcaa cttcgacctg gccgaggatg ccaaactgca
     5821 gctgagcaag gacacctacg acgacgacct ggacaacctg ctggcccaga tcggcgacca
     5881 gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac gccatcctgc tgagcgacat
     5941 cctgagagtg aacaccgaga tcaccaaggc ccccctgagc gcctctatga tcaagagata
     6001 cgacgagcac caccaggacc tgaccctgct gaaagctctc gtgcggcagc agctgcctga
     6061 gaagtacaaa gagattttct tcgaccagag caagaacggc tacgccggct acatcgatgg
     6121 cggagccagc caggaagagt tctacaagtt catcaagccc atcctggaaa agatggacgg
     6181 caccgaggaa ctgctcgtga agctgaacag agaggacctg ctgcggaagc agcggacctt
     6241 cgacaacggc agcatccccc accagatcca cctgggagag ctgcacgcca ttctgcggcg
     6301 gcaggaagat ttttacccat tcctgaagga caaccgggaa aagatcgaga agatcctgac
     6361 cttccgcatc ccctactacg tgggccctct ggccagggga aacagcagat tcgcctggat
     6421 gaccagaaag agcgaggaaa ccatcacccc ctggaacttc gaggaagtgg tggacaaggg
     6481 cgccagcgcc cagagcttca tcgagcggat gaccaacttc gataagaacc tgcccaacga
     6541 gaaggtgctg cccaagcaca gcctgctgta cgagtacttc accgtgtaca acgagctgac
     6601 caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc ttcctgagcg gcgagcagaa
     6661 aaaagccatc gtggacctgc tgttcaagac caaccggaaa gtgaccgtga agcagctgaa
     6721 agaggactac ttcaagaaaa tcgagtgctt cgactccgtg gaaatctccg gcgtggaaga
     6781 tcggttcaac gcctccctgg gcacatacca cgatctgctg aaaattatca aggacaagga
     6841 cttcctggac aatgaggaaa acgaggacat tctggaagat atcgtgctga ccctgacact
     6901 gtttgaggac agagagatga tcgaggaacg gctgaaaacc tatgcccacc tgttcgacga
     6961 caaagtgatg aagcagctga agcggcggag atacaccggc tggggcaggc tgagccggaa
     7021 gctgatcaac ggcatccggg acaagcagtc cggcaagaca atcctggatt tcctgaagtc
     7081 cgacggcttc gccaacagaa acttcatgca gctgatccac gacgacagcc tgacctttaa
     7141 agaggacatc cagaaagccc aggtgtccgg ccagggcgat agcctgcacg agcacattgc
     7201 caatctggcc ggcagccccg ccattaagaa gggcatcctg cagacagtga aggtggtgga
     7261 cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac atcgtgatcg aaatggccag
     7321 agagaaccag accacccaga agggacagaa gaacagccgc gagagaatga agcggatcga
     7381 agagggcatc aaagagctgg gcagccagat cctgaaagaa caccccgtgg aaaacaccca
     7441 gctgcagaac gagaagctgt acctgtacta cctgcagaat gggcgggata tgtacgtgga
     7501 ccaggaactg gacatcaacc ggctgtccga ctacgatgtg gacgctatcg tgcctcagag
     7561 ctttctgaag gacgactcca tcgataacaa agtgctgact cggagcgaca agaaccgggg
     7621 caagagcgac aacgtgccct ccgaagaggt cgtgaagaag atgaagaact actggcgcca
     7681 gctgctgaat gccaagctga ttacccagag gaagttcgac aatctgacca aggccgagag
     7741 aggcggcctg agcgaactgg ataaggccgg cttcatcaag agacagctgg tggaaacccg
     7801 gcagatcaca aagcacgtgg cacagatcct ggactcccgg atgaacacta agtacgacga
     7861 gaacgacaaa ctgatccggg aagtgaaagt gatcaccctg aagtccaagc tggtgtccga
     7921 tttccggaag gatttccagt tttacaaagt gcgcgagatc aacaactacc accacgccca
     7981 cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc aaaaagtacc ctaagctgga
     8041 aagcgagttc gtgtacggcg actacaaggt gtacgacgtg cggaagatga tcgccaagag
     8101 cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc tacagcaaca tcatgaactt
     8161 tttcaagacc gagattaccc tggccaacgg cgagatccgg aagcggcctc tgatcgagac
     8221 aaacggcgaa acaggcgaga tcgtgtggga taagggccgg gactttgcca ccgtgcggaa
     8281 agtgctgtct atgccccaag tgaatatcgt gaaaaagacc gaggtgcaga caggcggctt
     8341 cagcaaagag tctatcctgc ccaagaggaa cagcgacaag ctgatcgcca gaaagaagga
     8401 ctgggaccct aagaagtacg gcggcttcga cagccccacc gtggcctatt ctgtgctggt
     8461 ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag agtgtgaaag agctgctggg
     8521 gatcaccatc atggaaagaa gcagcttcga gaagaatccc atcgactttc tggaagccaa
     8581 gggctacaaa gaagtgaaaa aggacctgat catcaagctg cctaagtact ccctgttcga
     8641 gctggaaaac ggccggaaga gaatgctggc ctctgccggc gaactgcaga agggaaacga
     8701 actggccctg ccctccaaat atgtgaactt cctgtacctg gccagccact atgagaagct
     8761 gaagggctcc cccgaggata atgagcagaa acagctgttt gtggaacagc acaaacacta
     8821 cctggacgag atcatcgagc agatcagcga gttctccaag agagtgatcc tggccgacgc
     8881 taatctggac aaggtgctga gcgcctacaa caagcacaga gacaagccta tcagagagca
     8941 ggccgagaat atcatccacc tgtttaccct gaccaatctg ggagcccctg ccgccttcaa
     9001 gtactttgac accaccatcg accggaagag gtacaccagc accaaagagg tgctggacgc
     9061 caccctgatc caccagagca tcaccggcct gtacgagaca cggatcgacc tgtctcagct
     9121 gggaggcgac gcctatccct atgacgtgcc cgattatgcc agcctgggca gcggctcccc
     9181 caagaaaaaa cgcaaggtgg aagatcctaa gaaaaagcgg aaagtggaag atgctccaaa
     9241 gaagaagaga aaggtcgacg gcattggtag tgggagcaac ggcagcagcg gatcccttaa
     9301 gcttgccacc atgcgtacgg gcaagaatga gcaggaactg ctggaactgg acaaatgggc
     9361 aagcctgggg agcggctccg gaaagaacga gcaggaactg ctggaactgg acaagtgggc
     9421 ttctctgggc tccgggtccg ggaaaaacga acaggagctg ctggagctgg ataagtgggc
     9481 cagcctgggc tccgggtccg ggaagaatga acaggaactg ctggaactgg ataaatgggc
     9541 atctctgggc agcgggtccg gaaagaatga acaggagctg ctggaactgg acaagtgggc
     9601 atccctgggg agcgggtccg gaaagaatga acaggaactg ctggaactgg ataagtgggc
     9661 atccctggga agcggctccg gaaaaaacga gcaggagctg ctggagctgg acaagtgggc
     9721 ctctctgggc tccggctccg ggaagaatga acaggagctg ctggaactgg ataagtgggc
     9781 atctctgggg tccggaagcg gtaaaaacga gcaggaactg ctggaactgg acaagtgggc
     9841 aagcctgggg tctggctccg gcaaaaacga acaggaactg ctggaactgg acaaatgggc
     9901 cagcctgggc tccgggtccg ggaaaaatga gcaggaactg ctggaactgg ataaatgggc
     9961 gagcctgggc agcgggtccg gaaaaaacga acaggagctg ctggagctgg acaaatgggc
    10021 gagtctgggc tccggaagcg gcaagaatga acaggaactg ctggagctgg acaaatgggc
    10081 cagcctgggc agcggctccg gaaagaatga gcaggaactg ctggagctgg acaaatgggc
    10141 atctctgggc tccggctccg ggaaaaacga gcaggaactg ctggagctgg acaagtgggc
    10201 ttccctgggc agcggctccg gaaaaaatga acaggaactg ctggaactgg acaagtgggc
    10261 cagcctgggg tccgggtctg ggaagaacga gcaggaactg ctggagctgg acaagtgggc
    10321 atctctgggg tctggctctg gcaagaacga gcaggaactg ctggaactgg acaagtgggc
    10381 gagcctgggc agcgggtctg gcaagaatga acaggagctg ctggaactgg acaaatgggc
    10441 cagcctgggg tctgggtctg gaaagaatga gcaggaactg ctggagctgg acaagtgggc
    10501 atctctgggc tctgggtctg gcaagaacga gcaggagctg ctggagctgg ataagtgggc
    10561 atccctgggg tccgggtccg gcaagaatga acaggagctg ctggagctgg ataaatgggc
    10621 atccctgggc tctgggtctg ggaagaatga gcaggagctg ctggaactgg ataagtgggc
    10681 gagcctgggc agcgggtctg gcaagaatga gcaggaactg ctggagctgg acaagtgggc
    10741 ttctctgcac cggtcagcag tggaggacag cacgtaaccc gggcttatat gaagatgaag
    10801 atgaaatatt tggtgtgtca aataaaaagc ttgtgtgctt aagtttgtgt ttttttcttg
    10861 gcttgttgtg ttatgaattt gtggcttttt ctaatattaa atgaatgtaa gatcacatta
    10921 taatgaataa acaaatgttt ctataatcca ttgtgaatgt tttgttggat ctcttctgca
    10981 gcatataact actgtatgtg ctatggtatg gactatggaa tatgattaaa gataagcgct
    11041 gagctcggac ggcgcgacta gttttacgta cgttaattaa cccggggcgc gccgatatcg
    11101 agctctcccg gcgcgccgga gtggcagaca tactgtccca caaatgaaga tggaatctgt
    11161 aaaagaaaac gcgtgaaata atgcgtctga caaaggttag gtcggctgcc tttaatcaat
    11221 accaaagtgg tccctaccac gatggaaaaa ctgtgcagtc ggtttggctt tttctgacga
    11281 acaaataaga ttcgtggccg acaggtgggg gtccaccatg tgaaggcatc ttcagactcc
    11341 aataatggag caatgacgta agggcttacg aaataagtaa gggtagtttg ggaaatgtcc
    11401 actcacccgt cagtctataa atacttagcc cctccctcat tgttaaggga gcaaaatctc
    11461 agagagatag tcctagagag agaaagagag caagtagcct agaagtagtc aaggcggcga
    11521 agtattcagg cacgtggcca ggaagaagaa aagccaagac gacgaaaaca ggtaagagct
    11581 aagcttaatg gaggtgcagc tggtggaatc tgggggcgga ctggtgcagc ccgggggatc
    11641 tctgcggctg tcctgcgccg cctctggctc catctctagc gtggatgtga tgtcctggta
    11701 caggcaggcc cctggcaagc agagggaact ggtggccttc attactgata ggggaagaac
    11761 caattacaaa gtgagcgtga aaggccgctt cactatcagc cgggataatt ctaagaatat
    11821 ggtgtatctg cagatgaaca gcctgaagcc agaagacact gccgactatc tgtgcagggc
    11881 tgagtcccgg acttcctggt ccagcccatc tcctctggat gtgtgggggc gcggcactca
    11941 ggtgactgtg tcctccttgg atccaggtgg aggtggaagc ggtagcaaag gagaagaact
    12001 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
    12061 ttctgtccgt ggagagggtg aaggtgatgc tacaaacgga aaactcaccc ttaaatttat
    12121 ttgcactact ggaaaactac ctgttccgtg gccaacactt gtcactactc tgacctatgg
    12181 tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc
    12241 catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg ggacctacaa
    12301 gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg
    12361 tattgatttt aaagaagatg gaaacattct tggacacaaa ctcgagtaca actttaactc
    12421 acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
    12481 tcgccacaac gttgaagatg gttccgttca actagcagac cattatcaac aaaatactcc
    12541 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgtcct
    12601 ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc
    12661 tgggattaca catggcatgg atgagctcta caaaggtgga ggtcggaccg gtcgtacggg
    12721 cggtggcgga ggggatgctt tagacgattt tgacttagat atgcttggtt cagacgcgtt
    12781 agacgacttc gacctagaca tgttaggctc agatgcattg gacgacttcg atttagatat
    12841 gttgggctcc gatgccctag atgactttga tctagatatg ctaggtagtg gcggtggcgg
    12901 aggggctcca aagaagaaga gaaaggtccg tacgggagga ggatctcgga ccgaagagta
    12961 caagcttatc ctgaacggta aaaccctgaa aggtgaaacc accaccgaag ctgttgacgc
    13021 tgctaccgcg gaaaaagttt tcaaacagta cgctaacgac aacggtgttg acggtgaatg
    13081 gacctacgac gacgctacca aaaccttcac ggtaaccgaa ggtggtggta gcggtggtgg
    13141 tactagtcca aaaacaagga ggaggccgcg aagatcacaa cggaaaaggc cgcctacgcc
    13201 atggccgtaa gcttgtcatg ggtcgtttaa gctgccgatg tgcctgcgtc gtctggtgcc
    13261 ctctctccat atggaggttg tcaaagtatc tgctgttcgt gtcatgagtc gtgtcagtgt
    13321 tggtttaata atggaccggt tgtgttgtgt gtgcgtacta cccagaacta tgacaaatca
    13381 tgaataagtt tgatgtttga aattaaagcc tgtgctcatt atgttctgtc tttcagttgt
    13441 ctcctaatat ttgcctgcag gtactggcta tctaccgttt cttacttagg aggtgtttga
    13501 atgcactaaa actaatagtt agtggctaaa attagttaaa acatccaaac accatagcta
    13561 atagttgaac tattagctat ttttggaaaa ttagttaata gtgaggtagt tatttgttag
    13621 ctagctaatt caactaacaa tttttagcca actaacaatt agtttcagtg cattcaaaca
    13681 cccccttaat gttaacgtgg ttctatctac cgtgtcctaa tatatggttg attgttcggt
    13741 ttgttgctat gctattgggt tctgattgct gctagttctt gctgaatcca gaagttctcg
    13801 tagtatagct cagattcata ttatttattt gagtgataag tgatccaggt tattactatg
    13861 ttagctaggt tttttttaca aggataaatt atctgtgatc ataattctta tgaaagcttt
    13921 atgtttcctg gaggcagtgg catgcaatgc atgacagcaa cttgatcaca ccagctgagg
    13981 tagatacggt aacaaggttc ttaaatctgt tcaccaaatc attggagaac acacatacac
    14041 attcttgcca gtcttggtta gagaaatttc atgacaaaat gccaaagctg tcttgactct
    14101 tcacttttgg ccatgagtcg tgacttagtt tggtttaatg gaccggttct cctagcttgc
    14161 gctgagctca gtgtggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
    14221 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
    14281 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc
    14341 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
    14401 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
    14461 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
    14521 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
    14581 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
    14641 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
    14701 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
    14761 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
    14821 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
    14881 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
    14941 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
    15001 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
    15061 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
    15121 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
    15181 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
    15241 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
    15301 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
    15361 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
    15421 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
    15481 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cggctcaacc gtgcggctgc
    15541 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
    15601 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
    15661 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
    15721 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
    15781 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
    15841 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
    15901 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
    15961 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
    16021 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
    16081 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
    16141 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
    16201 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
    16261 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
    16321 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
    16381 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
    16441 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
    16501 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
    16561 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
    16621 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
    16681 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg cctgccggcc
    16741 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
    16801 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
    16861 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
    16921 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
    16981 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
    17041 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
    17101 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
    17161 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
    17221 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
    17281 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
    17341 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
    17401 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
    17461 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
    17521 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
    17581 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
    17641 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
    17701 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
    17761 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
    17821 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
    17881 gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac gtacattggg
    17941 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
    18001 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
    18061 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
    18121 ggccagcgca cagcccaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
    18181 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
    18241 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
    18301 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    18361 gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    18421 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
    18481 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
    18541 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggccc
    18601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    18661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    18721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    18781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    18841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    18901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    18961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    19021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    19081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    19141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    19201 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    19261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    19321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    19381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    19441 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
    19501 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
    19561 tcctc
//
