LOCUS       sequence-401206-        3836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Hudson LD, Berndt JA, Puckett C, Kozak CA, Lazzarini RA
  TITLE     Aberrant splicing of proteolipid protein mRNA in the dysmyelinating 
            jimpy mutant mouse.
  JOURNAL   Proc Natl Acad Sci U S A. 1987 Mar . 84(5):1454-8.
  PUBMED    3469678
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1987 Mar . 84(5):1454-8."
COMMENT     Sequence Label: pLH116
FEATURES             Location/Qualifiers
     source          1..3836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1082..1100)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1083..1100)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1390..1409)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1619..1638)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1738..1760
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1798..1816)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1884..1988
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1989..2057,2058..2849)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2207..2226)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3020..3608
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3509..3528
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3762..3779
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgcagct ggcttatcga aattaatacg actcactata gggagaccgg aattccgagg
       61 acaaagatac tcagagagaa aaagtaaagg acagaagaag gagactggag agaccaggat
      121 ccttccagct gagcaaagtc agccgcaaaa cagactagcc aacaggctac aattggagtc
      181 agagtgccaa agacatgggc ttgttagagt gttgtgctag atgtctggta ggggccccct
      241 ttgcttccct ggtggccact ggattgtgtt tctttggagt ggcactgttc tgtggatgtg
      301 gacatgaagc tctcactggt acagaaaagc taattgagac ctatttctcc aaaaactacc
      361 aggactatga gtatctcatt aatgtgattc atgctttcca gtatgtcatc tatggaactg
      421 cctctttctt cttcctttat ggggccctcc tgctggctga gggcttctac accaccggcg
      481 ctgtcaggca gatctttggc gactacaaga ccaccatctg cggcaagggc ctgagcgcaa
      541 cggtaacagg gggccagaag gggaggggtt ccagaggcca acatcaagct cattctttgg
      601 agcgggtgtg tcattgtttg ggaaaatggc taggacatcc cgacaagttt gtgggcatca
      661 cctatgccct gactgttgta tggctcctgg tgtttgcctg ctcggctgta cctgtgtaca
      721 tttacttcaa tacctggacc acctgtcagt ctattgcctt ccctagcaag acctctgcca
      781 gtataggcag tctctgcgct gatgccagaa tgtatggtgt tctcccatgg aatgctttcc
      841 ctggcaaggt ttgtggctcc aaccttctgt ccatctgcaa aacagctgag ttccaaatga
      901 ccttccacct gtttattgct gcgtttgtgg gtgctgcggc cacactagtt tccctgctca
      961 ccttcatgat tgctgccact tacaacttcg ccgtccttaa actcatgggc cgaggcacca
     1021 agttctgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttgta
     1081 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     1141 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     1201 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     1261 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     1321 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     1381 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     1441 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     1501 gcgccatctc cttgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     1561 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
     1621 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     1681 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     1741 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     1801 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     1861 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     1921 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     1981 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     2041 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     2101 tgggtgcacg agtggggtac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     2161 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     2221 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     2281 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     2341 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     2401 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     2461 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     2521 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     2581 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     2641 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     2701 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     2761 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     2821 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     2881 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     2941 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     3001 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     3061 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3241 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3301 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3361 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3421 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3481 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3541 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3601 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3661 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3721 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3781 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcat
//
