LOCUS       sequence-401214-        2353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_59 [TtetL]
FEATURES             Location/Qualifiers
     source          1..2353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(397..413)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(397..413)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(410..432)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(585..1173)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(665..684)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1306..1400)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1421..2080)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1995..2014
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2081..2183)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2270..2292
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2284..2301
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2285..2301
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gcttatcgtt aagggatcaa ctttgggaga gagttcaaaa
      121 ttgatccttt ttttacgctt gagaccaccc caggatacat agattaccac aactccgagc
      181 ccttccacct agcgtgctaa ccacgcacgc tattgttgta tcccatataa tgcaatcgta
      241 gtctacctga ctgtacttag aaatgtggct tcgcctttgc ccacgcacct gatcgctcct
      301 cgtttgcttt taaggaccgg acgaaccaca gagcattaga aggctaggtg gaggctcagt
      361 gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa
      421 ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct ccaagacatt
      481 aggtggagtt cagttcggcg tatggcatat gtcgctggaa agaacatgtg agcaaaaggc
      541 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
      601 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
      661 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
      721 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
      781 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
      841 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
      901 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
      961 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1021 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1081 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1141 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1201 tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc
     1261 ttcaaatcgt ccccccatac gatataagtt gttactagtg cttggattct caccaataaa
     1321 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1381 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1441 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1501 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     1561 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     1621 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     1681 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     1741 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     1801 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     1861 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     1921 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     1981 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2041 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2101 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     2161 tggaacctct tacgtgccga tcaacgtctc attgatacct gaaacaaaac ccatcgtacg
     2221 gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc ccagtcacga
     2281 cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca
     2341 ttccgcctga cct
//
