LOCUS       sequence-401215-        2420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_28 [Paph(3')-I EZ-Tn5]
FEATURES             Location/Qualifiers
     source          1..2420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(464..480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(464..480)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(477..499)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(652..1240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(732..751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1373..1467)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1488..2147)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2062..2081
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2148..2250)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2337..2359
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2351..2368
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2352..2368
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggagcaacca tcatcgatga attgtgtctc aaaatctctg
      121 atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt ctgcttacat
      181 aaacagtaat acaaggggtg ttaatgtgag accaccccag gatacataga ttaccacaac
      241 tccgagccct tccacctagc gtgctaacca cgcacgctat tgttgtatcc catataatgc
      301 aatcgtagtc tacctgactg tacttagaaa tgtggcttcg cctttgccca cgcacctgat
      361 cgctcctcgt ttgcttttaa ggaccggacg aaccacagag cattagaagg ctaggtggag
      421 gctcagtgat gataagtctg cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg
      481 tgtgaaattg ttatccgctc agagggcaca atcctattcc gcgctatccg acaatctcca
      541 agacattagg tggagttcag ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc
      601 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      661 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      721 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      781 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      841 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      901 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      961 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1021 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1081 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1141 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1201 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1261 tacggggtct gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt
     1321 agggggcttc aaatcgtccc cccatacgat ataagttgtt actagtgctt ggattctcac
     1381 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     1441 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     1501 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1561 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1621 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1681 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     1741 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     1801 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     1861 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     1921 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1981 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     2041 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     2101 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     2161 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     2221 tgaaagttgg aacctcttac gtgccgatca acgtctcatt gatacctgaa acaaaaccca
     2281 tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca
     2341 gtcacgacgt tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga
     2401 agtgccattc cgcctgacct
//
