LOCUS       sequence-401216-        2374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_30 [PaphA-3 ]
FEATURES             Location/Qualifiers
     source          1..2374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(418..434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(418..434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(431..453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(606..1194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(686..705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1327..1421)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1442..2101)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2016..2035
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2102..2204)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2291..2313
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2305..2322
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2306..2322
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggaggggatc ggattcgagc taagaattcc cttggggtat
      121 ctttaaatac tgtagaaaag aggaaggaaa taataaaatg tgagaccacc ccaggataca
      181 tagattacca caactccgag cccttccacc tagcgtgcta accacgcacg ctattgttgt
      241 atcccatata atgcaatcgt agtctacctg actgtactta gaaatgtggc ttcgcctttg
      301 cccacgcacc tgatcgctcc tcgtttgctt ttaaggaccg gacgaaccac agagcattag
      361 aaggctaggt ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc
      421 atagctgttt cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta
      481 tccgacaatc tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga
      541 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      601 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      661 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      721 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      781 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      841 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      901 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      961 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1021 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1081 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1141 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1201 cctttgatct tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt
     1261 cagcgtaaat gggtaggggg cttcaaatcg tccccccata cgatataagt tgttactagt
     1321 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1381 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1441 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1501 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1561 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1621 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     1681 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1741 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1801 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1861 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1921 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1981 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2041 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2101 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2161 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattgatacc
     2221 tgaaacaaaa cccatcgtac ggccaaggaa gtctccaata actgtgatcc accacaagcg
     2281 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtcatgcata atccgcacgc
     2341 atctggaata aggaagtgcc attccgcctg acct
//
