LOCUS       sequence-401229-        2869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_45 [nat1]
FEATURES             Location/Qualifiers
     source          1..2869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(913..929)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(913..929)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(926..948)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1101..1689)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1181..1200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1822..1916)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1937..2596)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2511..2530
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2597..2699)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2786..2808
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2800..2817
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2801..2817
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca aatgaccact ctggatgaca ccgcttaccg ataccgaact
      121 tccgttcctg gcgatgccga ggctattgag gctctggatg gatctttcac cactgacacc
      181 gttttccgag tgaccgctac tggcgacggc ttcaccctgc gagaggtgcc tgtcgaccct
      241 cctctcacca aggttttccc tgacgatgag tcggacgatg agtctgacgc tggagaggac
      301 ggcgaccctg actctcgaac tttcgtggct tacggcgacg atggcgacct ggccggcttt
      361 gtggtcgttt cttactccgg atggaaccga cgactgaccg tggaggacat cgaggtcgct
      421 cctgagcacc gaggtcatgg tgtcggacga gctctgatgg gtctggctac tgagttcgct
      481 cgagagcgag gtgctggcca cctgtggctc gaggtcacca acgttaacgc ccctgctatt
      541 catgcctacc gacgaatggg ttttaccctg tgtggcctcg atactgccct gtacgacgga
      601 accgcttccg atggagagca ggccctctac atgtcgatgc cctgccctta agctttgaga
      661 ccaccccagg atacatagat taccacaact ccgagccctt ccacctagcg tgctaaccac
      721 gcacgctatt gttgtatccc atataatgca atcgtagtct acctgactgt acttagaaat
      781 gtggcttcgc ctttgcccac gcacctgatc gctcctcgtt tgcttttaag gaccggacga
      841 accacagagc attagaaggc taggtggagg ctcagtgatg ataagtctgc gatggtggat
      901 gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca gagggcacaa
      961 tcctattccg cgctatccga caatctccaa gacattaggt ggagttcagt tcggcgtatg
     1021 gcatatgtcg ctggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1081 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1141 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1201 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1261 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1321 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1381 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1441 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1501 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1561 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1621 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1681 ggatctcaag aagatccttt gatcttttct acggggtctg acgctctatt caacaaagcc
     1741 gccgtcccgt caagtcagcg taaatgggta gggggcttca aatcgtcccc ccatacgata
     1801 taagttgtta ctagtgcttg gattctcacc aataaaaaac gcccggcggc aaccgagcgt
     1861 tctgaacaaa tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag
     1921 cgagctcgat atcaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat
     1981 taagcattct gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg
     2041 gcatcagcac cttgtcgcct tgcgtataat atttgcccat ggtgaaaacg ggggcgaaga
     2101 agttgtccat attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg
     2161 agacgaaaaa catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac
     2221 acgccacatc ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc
     2281 agagcgatga aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat
     2341 cccatatcac cagctcaccg tctttcattg ccatacgaaa ttccggatga gcattcatca
     2401 ggcgggcaag aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct
     2461 ttaaaaaggc cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact
     2521 gaaatgcctc aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag
     2581 tgattttttt ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata
     2641 cgcccggtag tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa
     2701 cgtctcattg atacctgaaa caaaacccat cgtacggcca aggaagtctc caataactgt
     2761 gatccaccac aagcgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtcat
     2821 gcataatccg cacgcatctg gaataaggaa gtgccattcc gcctgacct
//
