LOCUS       sequence-401230-        3034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_55 [ermC]
FEATURES             Location/Qualifiers
     source          1..3034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1078..1094)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1078..1094)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1091..1113)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1266..1854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1346..1365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1987..2081)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2102..2761)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2676..2695
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2762..2864)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2951..2973
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2965..2982
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2966..2982
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca aatgaacgag aaaaatataa aacacagtca aaactttatt
      121 acttcaaaac ataatataga taaaataatg acaaatataa gattaaatga acatgataat
      181 atctttgaaa tcggctcagg aaaaggccat tttacccttg aattagtaaa gaggtgtaat
      241 ttcgtaactg ccattgaaat agaccataaa ttatgcaaaa ctacagaaaa taaacttgtt
      301 gatcacgata atttccaagt tttaaacaag gatatattgc agtttaaatt tcctaaaaac
      361 caatcctata aaatatatgg taatatacct tataacataa gtacggatat aatacgcaaa
      421 attgtttttg atagtatagc taatgagatt tatttaatcg tggaatacgg gtttgctaaa
      481 agattattaa atacaaaacg ctcattggca ttacttttaa tggcagaagt tgatatttct
      541 atattaagta tggttccaag agaatatttt catcctaaac ctaaagtgaa tagctcactt
      601 atcagattaa gtagaaaaaa atcaagaata tcacacaaag ataaacaaaa gtataattat
      661 ttcgttatga aatgggttaa caaagaatac aagaaaatat ttacaaaaaa tcaatttaac
      721 aattccttaa aacatgcagg aattgacgat ttaaacaata ttagctttga acaattctta
      781 tctcttttca atagctataa attatttaat aagtaagctt tgagaccacc ccaggataca
      841 tagattacca caactccgag cccttccacc tagcgtgcta accacgcacg ctattgttgt
      901 atcccatata atgcaatcgt agtctacctg actgtactta gaaatgtggc ttcgcctttg
      961 cccacgcacc tgatcgctcc tcgtttgctt ttaaggaccg gacgaaccac agagcattag
     1021 aaggctaggt ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc
     1081 atagctgttt cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta
     1141 tccgacaatc tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga
     1201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1861 cctttgatct tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt
     1921 cagcgtaaat gggtaggggg cttcaaatcg tccccccata cgatataagt tgttactagt
     1981 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     2041 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     2101 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     2161 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2221 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2281 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2341 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2401 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2461 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2521 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     2581 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2641 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2701 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2761 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2821 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattgatacc
     2881 tgaaacaaaa cccatcgtac ggccaaggaa gtctccaata actgtgatcc accacaagcg
     2941 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtcatgcata atccgcacgc
     3001 atctggaata aggaagtgcc attccgcctg acct
//
