LOCUS       sequence-401231-        2378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_36 [PtetA pSC101]
FEATURES             Location/Qualifiers
     source          1..2378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(85..90,91..107,108..113)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(422..438)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(422..438)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(435..457)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(610..1198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(690..709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1331..1425)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1446..2105)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2020..2039
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2106..2208)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2295..2317
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2309..2326
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2310..2326
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggagttgaca gcttatcatc gataagcttt aatgcggtag
      121 tttatcacag ttaaattgct aacgcagtca ggcaccgtgt aatgtgagac caccccagga
      181 tacatagatt accacaactc cgagcccttc cacctagcgt gctaaccacg cacgctattg
      241 ttgtatccca tataatgcaa tcgtagtcta cctgactgta cttagaaatg tggcttcgcc
      301 tttgcccacg cacctgatcg ctcctcgttt gcttttaagg accggacgaa ccacagagca
      361 ttagaaggct aggtggaggc tcagtgatga taagtctgcg atggtggatg catgtgtcat
      421 ggtcatagct gtttcctgtg tgaaattgtt atccgctcag agggcacaat cctattccgc
      481 gctatccgac aatctccaag acattaggtg gagttcagtt cggcgtatgg catatgtcgc
      541 tggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     1021 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     1081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     1141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     1201 agatcctttg atcttttcta cggggtctga cgctctattc aacaaagccg ccgtcccgtc
     1261 aagtcagcgt aaatgggtag ggggcttcaa atcgtccccc catacgatat aagttgttac
     1321 tagtgcttgg attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat
     1381 ccagatggag ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata
     1441 tcaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1501 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1561 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1621 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1681 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1741 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1801 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1861 agctcaccgt ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga
     1921 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1981 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     2041 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     2101 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     2161 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcattga
     2221 tacctgaaac aaaacccatc gtacggccaa ggaagtctcc aataactgtg atccaccaca
     2281 agcgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtcatg cataatccgc
     2341 acgcatctgg aataaggaag tgccattccg cctgacct
//
