LOCUS       sequence-401238-        2635 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_19 [panARS ORI]
FEATURES             Location/Qualifiers
     source          1..2635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(74..733)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     648..667
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(734..836)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     923..945
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     937..954
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     938..954
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1685..1701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1685..1701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1698..1720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1873..2461)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1953..1972)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(2594..2635,1..53))
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
       61 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
      121 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
      181 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
      241 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaga
      301 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
      361 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
      421 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
      481 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
      541 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
      601 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
      661 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
      721 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
      781 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt
      841 ctcattgata cctgaaacaa aacccatcgt acggccaagg aagtctccaa taactgtgat
      901 ccaccacaag cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtcatgca
      961 taatccgcac gcatctggaa taaggaagtg ccattccgcc tgaccttagc gtgcgaacag
     1021 cacgctattg ttgtatcatt actcgcatcc attctcaggc tgtctcgtct cgtctccgag
     1081 gtctccggag tcaacatctt tggataatat cagaatgaga aagaacagat acgcagtacg
     1141 ttttttggtg agctctttgc acttctttag ttctttccat caatatcagt tgcttatgca
     1201 cttatgacta atattgatgt ttaacttcaa tatctttaaa cttttgttct tcccgacgtt
     1261 cattaagaat actaatacac tttaataatt agtttaatat ttgtttctat ataatgacat
     1321 ttaattaaaa aagataaaat ataaaaacat cataataact caccagaggt taagaacaaa
     1381 aaaacaaatt agatatctgc taatccaata tagttaaatc aatctttcct tggtataatg
     1441 ggtatattac atatatttca aggaccgaca ctcctaccaa atatctaaaa tttaccatat
     1501 taacataaca tgtatataaa cgtcaaatca taatcagcac tacgctcgag accaccccag
     1561 gatacataga ttaccacaac tccgagccct tccacctagc gtgctaacca cgcacgctat
     1621 tgttgtatag gctaggtgga ggctcagtga tgataagtct gcgatggtgg atgcatgtgt
     1681 catggtcata gctgtttcct gtgtgaaatt gttatccgct cagagggcac aatcctattc
     1741 cgcgctatcc gacaatctcc aagacattag gtggagttca gttcggcgta tggcatatgt
     1801 cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1861 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1921 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1981 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2041 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2101 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2161 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2221 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2281 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2341 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2401 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2461 agaagatcct ttgatctttt ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc
     2521 gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc ccccatacga tataagttgt
     2581 tactagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttct
//
