LOCUS       sequence-401239-        2674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_48 [ble]
FEATURES             Location/Qualifiers
     source          1..2674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(718..734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(718..734)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(731..753)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(906..1494)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(986..1005)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1627..1721)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1742..2401)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2316..2335
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2402..2504)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2591..2613
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2605..2622
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2606..2622
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca aatggctaaa ttaacatctg ccgttcctgt tttaacagct
      121 agggatgttg caggtgctgt agagttttgg acagataggt taggattctc aagagacttt
      181 gttgaggacg attttgctgg tgttgtcagg gatgacgtta ctttatttat ctcagcagtc
      241 caagatcaag ttgtccctga taatacattg gcttgggtct gggtcagggg tttagatgaa
      301 ttatatgctg aatggtcaga agttgtatct acaaacttca gagatgcttc tggtccagct
      361 atgaccgaga ttggtgaaca gccatggggt agagaatttg ctttgagaga tccagctgga
      421 aattgtgttc attttgttgc tgaagaacaa gattaagctt tgagaccacc ccaggataca
      481 tagattacca caactccgag cccttccacc tagcgtgcta accacgcacg ctattgttgt
      541 atcccatata atgcaatcgt agtctacctg actgtactta gaaatgtggc ttcgcctttg
      601 cccacgcacc tgatcgctcc tcgtttgctt ttaaggaccg gacgaaccac agagcattag
      661 aaggctaggt ggaggctcag tgatgataag tctgcgatgg tggatgcatg tgtcatggtc
      721 atagctgttt cctgtgtgaa attgttatcc gctcagaggg cacaatccta ttccgcgcta
      781 tccgacaatc tccaagacat taggtggagt tcagttcggc gtatggcata tgtcgctgga
      841 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      901 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      961 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1021 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1081 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1141 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1201 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1261 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1321 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1381 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1441 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1501 cctttgatct tttctacggg gtctgacgct ctattcaaca aagccgccgt cccgtcaagt
     1561 cagcgtaaat gggtaggggg cttcaaatcg tccccccata cgatataagt tgttactagt
     1621 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1681 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1741 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1801 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1861 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1921 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     1981 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2041 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2101 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2161 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     2221 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2281 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2341 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2401 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2461 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattgatacc
     2521 tgaaacaaaa cccatcgtac ggccaaggaa gtctccaata actgtgatcc accacaagcg
     2581 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtcatgcata atccgcacgc
     2641 atctggaata aggaagtgcc attccgcctg acct
//
