LOCUS       sequence-401241-        2435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_101 [PnptII]
FEATURES             Location/Qualifiers
     source          1..2435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(295..389)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(410..1069)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     984..1003
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1070..1172)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     1259..1281
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1273..1290
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1274..1290
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1821..1837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1821..1837)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1834..1856)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(join(2009..2435,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2089..2108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctct attcaacaaa gccgccgtcc cgtcaagtca gcgtaaatgg
      241 gtagggggct tcaaatcgtc cccccatacg atataagttg ttactagtgc ttggattctc
      301 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
      361 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
      421 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
      481 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
      541 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
      601 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
      661 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
      721 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
      781 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
      841 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
      901 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
      961 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     1021 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     1081 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
     1141 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttgatacctg aaacaaaacc
     1201 catcgtacgg ccaaggaagt ctccaataac tgtgatccac cacaagcgcc agggttttcc
     1261 cagtcacgac gttgtaaaac gacggccagt catgcataat ccgcacgcat ctggaataag
     1321 gaagtgccat tccgcctgac cttagcgtgc gaacagcacg ctattgttgt atcattactc
     1381 gcatccattc tcaggctgtc tcgtctcgtc tccgaggtct ccggagactg ggctatctgg
     1441 acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga
     1501 tagctagact gggcggtctt atggacagca agcgaaccgg aattgccagc tggggcgccc
     1561 tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc
     1621 tgatggcgca ggggatcaag atctgatcaa gagacaggat actagtggag gaagaaaaaa
     1681 tgcgagacca ccccaggata catagattac cacaactccg agcccttcca cctagcgtgc
     1741 taaccacgca cgctattgtt gtataggcta ggtggaggct cagtgatgat aagtctgcga
     1801 tggtggatgc atgtgtcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaga
     1861 gggcacaatc ctattccgcg ctatccgaca atctccaaga cattaggtgg agttcagttc
     1921 ggcgtatggc atatgtcgct ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1981 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2041 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2101 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2161 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2221 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2281 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2341 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2401 gtgctacaga gttcttgaag tggtggccta actac
//
