LOCUS       sequence-401244-        2360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_107 [TAmarelleT7]
FEATURES             Location/Qualifiers
     source          1..2360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(404..420)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(404..420)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(417..439)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(592..1180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(672..691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1313..1407)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1428..2087)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2002..2021
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2088..2190)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2277..2299
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2291..2308
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2292..2308
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gcttcgctcg tgctcgccgc gaccttcctc ttcatgcacc
      121 tcttcggcat cgggctgcac aacgcttgag accaccccag gatacataga ttaccacaac
      181 tccgagccct tccacctagc gtgctaacca cgcacgctat tgttgtatcc catataatgc
      241 aatcgtagtc tacctgactg tacttagaaa tgtggcttcg cctttgccca cgcacctgat
      301 cgctcctcgt ttgcttttaa ggaccggacg aaccacagag cattagaagg ctaggtggag
      361 gctcagtgat gataagtctg cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg
      421 tgtgaaattg ttatccgctc agagggcaca atcctattcc gcgctatccg acaatctcca
      481 agacattagg tggagttcag ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc
      541 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      601 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      661 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      721 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      781 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      841 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      901 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      961 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1021 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1081 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     1141 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1201 tacggggtct gacgctctat tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt
     1261 agggggcttc aaatcgtccc cccatacgat ataagttgtt actagtgctt ggattctcac
     1321 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     1381 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     1441 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     1501 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     1561 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     1621 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
     1681 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     1741 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     1801 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     1861 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     1921 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     1981 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     2041 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     2101 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     2161 tgaaagttgg aacctcttac gtgccgatca acgtctcatt gatacctgaa acaaaaccca
     2221 tcgtacggcc aaggaagtct ccaataactg tgatccacca caagcgccag ggttttccca
     2281 gtcacgacgt tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga
     2341 agtgccattc cgcctgacct
//
