LOCUS       sequence-401245-        2833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_39 [aac(3)-Ia]
FEATURES             Location/Qualifiers
     source          1..2833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             82..615
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     complement(877..893)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(877..893)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(890..912)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1065..1653)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1145..1164)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1786..1880)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1901..2560)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2475..2494
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2561..2663)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2750..2772
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2764..2781
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2765..2781
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca aatgttacgc agcagcaacg atgttacgca gcagggcagt
      121 cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg taggctcggc
      181 cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga gttcggagac
      241 gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt gctccgtagt
      301 aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc tctcgcggct
      361 tacgttctgc ccaagtttga gcagccgcgt agtgagatct atatctatga tctcgcagtc
      421 tccggagagc accggaggca gggcattgcc accgcgctca tcaatctcct caagcatgag
      481 gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga cgatcccgca
      541 gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga tatcgaccca
      601 agtaccgcca cctaagcttt gagaccaccc caggatacat agattaccac aactccgagc
      661 ccttccacct agcgtgctaa ccacgcacgc tattgttgta tcccatataa tgcaatcgta
      721 gtctacctga ctgtacttag aaatgtggct tcgcctttgc ccacgcacct gatcgctcct
      781 cgtttgcttt taaggaccgg acgaaccaca gagcattaga aggctaggtg gaggctcagt
      841 gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc ctgtgtgaaa
      901 ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct ccaagacatt
      961 aggtggagtt cagttcggcg tatggcatat gtcgctggaa agaacatgtg agcaaaaggc
     1021 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1081 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1141 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     1201 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     1261 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     1321 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     1381 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     1441 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     1501 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     1561 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     1621 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     1681 tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg ggtagggggc
     1741 ttcaaatcgt ccccccatac gatataagtt gttactagtg cttggattct caccaataaa
     1801 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1861 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1921 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1981 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     2041 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2101 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     2161 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2221 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2281 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
     2341 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
     2401 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
     2461 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
     2521 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
     2581 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     2641 tggaacctct tacgtgccga tcaacgtctc attgatacct gaaacaaaac ccatcgtacg
     2701 gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc ccagtcacga
     2761 cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca
     2821 ttccgcctga cct
//
