LOCUS       sequence-401246-        2431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_109 [TAmarelleT12]
FEATURES             Location/Qualifiers
     source          1..2431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(475..491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(475..491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(488..510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(663..1251)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(743..762)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1384..1478)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1499..2158)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2073..2092
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2159..2261)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2348..2370
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2362..2379
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2363..2379
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gcttctcgct ctatctccat cacccgaccg cgaccgaccc
      121 aaaaatggcg ccgccgggcc attcgacctt ctacgcgctc gcgccggtcc cccatctcgg
      181 caaattcccc gtcgactggg cgcgcgtcgg gcccgcttga gaccacccca ggatacatag
      241 attaccacaa ctccgagccc ttccacctag cgtgctaacc acgcacgcta ttgttgtatc
      301 ccatataatg caatcgtagt ctacctgact gtacttagaa atgtggcttc gcctttgccc
      361 acgcacctga tcgctcctcg tttgctttta aggaccggac gaaccacaga gcattagaag
      421 gctaggtgga ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata
      481 gctgtttcct gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc
      541 gacaatctcc aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag
      601 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      661 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      721 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      781 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      841 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      901 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      961 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     1021 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     1081 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     1141 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     1201 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     1261 ttgatctttt ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag
     1321 cgtaaatggg tagggggctt caaatcgtcc ccccatacga tataagttgt tactagtgct
     1381 tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg
     1441 gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg atatcaaatt
     1501 acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat
     1561 ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc
     1621 cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca
     1681 cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct
     1741 caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat
     1801 atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt
     1861 cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac
     1921 cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca agaatgtgaa
     1981 taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat
     2041 ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt
     2101 ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt
     2161 tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta
     2221 tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tgatacctga
     2281 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
     2341 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
     2401 tggaataagg aagtgccatt ccgcctgacc t
//
