LOCUS       sequence-401249-        2460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_106 [TAmarelleT1]
FEATURES             Location/Qualifiers
     source          1..2460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(504..520)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(504..520)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(517..539)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(692..1280)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(772..791)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1413..1507)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1528..2187)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2102..2121
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2188..2290)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2377..2399
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2391..2408
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2392..2408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca gcttgagcgc atgctcgagt acttcgcctg gaccatgctc
      121 gccgtcgtct tcggcttcct cctcttcgtc aacctcgcct acgtccccct cggccactgg
      181 gccgaggcct tcgccggctt cttcaaattt tcagggctgc cgcaccccat cgactggggg
      241 ctcgcttgag accaccccag gatacataga ttaccacaac tccgagccct tccacctagc
      301 gtgctaacca cgcacgctat tgttgtatcc catataatgc aatcgtagtc tacctgactg
      361 tacttagaaa tgtggcttcg cctttgccca cgcacctgat cgctcctcgt ttgcttttaa
      421 ggaccggacg aaccacagag cattagaagg ctaggtggag gctcagtgat gataagtctg
      481 cgatggtgga tgcatgtgtc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      541 agagggcaca atcctattcc gcgctatccg acaatctcca agacattagg tggagttcag
      601 ttcggcgtat ggcatatgtc gctggaaaga acatgtgagc aaaaggccag caaaaggcca
      661 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      721 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      781 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      841 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      901 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      961 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     1021 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     1081 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     1141 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     1201 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1261 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctctat
     1321 tcaacaaagc cgccgtcccg tcaagtcagc gtaaatgggt agggggcttc aaatcgtccc
     1381 cccatacgat ataagttgtt actagtgctt ggattctcac caataaaaaa cgcccggcgg
     1441 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     1501 aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat cgcagtactg
     1561 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
     1621 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
     1681 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
     1741 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
     1801 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
     1861 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
     1921 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg
     1981 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
     2041 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
     2101 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
     2161 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa
     2221 ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac
     2281 gtgccgatca acgtctcatt gatacctgaa acaaaaccca tcgtacggcc aaggaagtct
     2341 ccaataactg tgatccacca caagcgccag ggttttccca gtcacgacgt tgtaaaacga
     2401 cggccagtca tgcataatcc gcacgcatct ggaataagga agtgccattc cgcctgacct
//
