LOCUS       sequence-401252-        2663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_104 [pAnTrpC]
FEATURES             Location/Qualifiers
     source          1..2663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(707..723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(707..723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(720..742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(895..1483)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(975..994)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1616..1710)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1731..2390)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2305..2324
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2391..2493)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2580..2602
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2594..2611
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2595..2611
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggagaactga tattgaagga gcattttttg ggcttggctg
      121 gagctagtgg aggtcaacaa tgaatgccta ttttggttta gtcgtccagg cggtgagcac
      181 aaaatttgtg tcgtttgaca agatggttca tttaggcaac tggtcagatc agccccactt
      241 gtagcagtag cggcggcgct cgaagtgtga ctcttattag cagacaggaa cgaggacatt
      301 attatcatct gctgcttggt gcacgataac ttggtgcgtt tgtcaagcaa ggtaagtgga
      361 cgacccggtc ataccttctt aagttcgccc ttcctccctt tatttcagat tcaatctgac
      421 ttacctattc tacccaagca tccaaaatgt gagaccaccc caggatacat agattaccac
      481 aactccgagc ccttccacct agcgtgctaa ccacgcacgc tattgttgta tcccatataa
      541 tgcaatcgta gtctacctga ctgtacttag aaatgtggct tcgcctttgc ccacgcacct
      601 gatcgctcct cgtttgcttt taaggaccgg acgaaccaca gagcattaga aggctaggtg
      661 gaggctcagt gatgataagt ctgcgatggt ggatgcatgt gtcatggtca tagctgtttc
      721 ctgtgtgaaa ttgttatccg ctcagagggc acaatcctat tccgcgctat ccgacaatct
      781 ccaagacatt aggtggagtt cagttcggcg tatggcatat gtcgctggaa agaacatgtg
      841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1081 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1141 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1201 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1261 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1321 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1381 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1441 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1501 ttctacgggg tctgacgctc tattcaacaa agccgccgtc ccgtcaagtc agcgtaaatg
     1561 ggtagggggc ttcaaatcgt ccccccatac gatataagtt gttactagtg cttggattct
     1621 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
     1681 aggtcattac tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg
     1741 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1801 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1861 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1921 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     1981 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     2041 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     2101 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     2161 attgccatac gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     2221 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     2281 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     2341 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     2401 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta
     2461 tggtgaaagt tggaacctct tacgtgccga tcaacgtctc attgatacct gaaacaaaac
     2521 ccatcgtacg gccaaggaag tctccaataa ctgtgatcca ccacaagcgc cagggttttc
     2581 ccagtcacga cgttgtaaaa cgacggccag tcatgcataa tccgcacgca tctggaataa
     2641 ggaagtgcca ttccgcctga cct
//
