LOCUS       sequence-401254-        2681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z,
            Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
  TITLE     A scalable framework for high-throughput identification of 
            functional origins of replication in non-model bacteria
  JOURNAL   bioRxiv 2023.05.19.541510
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.19.541510"
COMMENT     Sequence Label: pGL0_102 [pAgTEF1]
FEATURES             Location/Qualifiers
     source          1..2681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(725..741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(725..741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(738..760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(913..1501)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(993..1012)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1634..1728)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1749..2408)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2323..2342
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2409..2511)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     2598..2620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2612..2629
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2613..2629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tagcgtgcga acagcacgct attgttgtat cattactcgc atccattctc aggctgtctc
       61 gtctcgtctc cgaggtctca ggagagcttg cctcgtcccc gccgggtcac ccggccagcg
      121 acatggaggc ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat
      181 gtgactgtcg cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac
      241 attttgatgg ccgcacggcg cgaagcaaaa attacggctc ctcgctccag acctgcgagc
      301 agggaaacgc tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa
      361 atataaaagg ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct
      421 tgctaggata cagttctcac atcacatccg aacataaaca aaaaatgtga gaccacccca
      481 ggatacatag attaccacaa ctccgagccc ttccacctag cgtgctaacc acgcacgcta
      541 ttgttgtatc ccatataatg caatcgtagt ctacctgact gtacttagaa atgtggcttc
      601 gcctttgccc acgcacctga tcgctcctcg tttgctttta aggaccggac gaaccacaga
      661 gcattagaag gctaggtgga ggctcagtga tgataagtct gcgatggtgg atgcatgtgt
      721 catggtcata gctgtttcct gtgtgaaatt gttatccgct cagagggcac aatcctattc
      781 cgcgctatcc gacaatctcc aagacattag gtggagttca gttcggcgta tggcatatgt
      841 cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      901 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      961 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1021 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1081 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     1141 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1201 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1261 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1321 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1381 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1441 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1501 agaagatcct ttgatctttt ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc
     1561 gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc ccccatacga tataagttgt
     1621 tactagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     1681 aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca agcgagctcg
     1741 atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     1801 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     1861 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     1921 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     1981 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     2041 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     2101 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     2161 accagctcac cgtctttcat tgccatacga aattccggat gagcattcat caggcgggca
     2221 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     2281 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     2341 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     2401 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     2461 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     2521 tgatacctga aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc
     2581 acaagcgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc
     2641 cgcacgcatc tggaataagg aagtgccatt ccgcctgacc t
//
